INT1 inositol transporter 1 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: INT1
Official Full Name: inositol transporter 1
Primary source: TAIR:AT2G43330
Locus tag: AT2G43330
See related: Araport:AT2G43330
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ATINT1; inositol transporter 1; INOSITOL TRANSPORTER 1; T1O24.7
Summary: Encodes a tonoplast-localized myo-inositol exporter, involved in efflux of myo-inositol from the vacuole to the cytosol. The gene is ubiquitously expressed. Reduced root growth in knock-out mutants grown on low inositol agar medium.
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Genomic context

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See INT1 in Genome Data Viewer

Location:: chromosome: 2

Exon count:: 5

Sequence:: Chromosome: 2; NC_003071.7 (18000822..18004195)

Chromosome 2 - NC_003071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003071.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

INT1 is correctly localized to the tonoplast, while sorting of the vacuolar sucrose transporter SUC4 is blocked in cis-Golgi stacks.
Title: Routes to the tonoplast: the sorting of tonoplast transporters in Arabidopsis mesophyll protoplasts.
Mutation in INT1 impairs abscisic acid signaling which leads to the reduction of reactive oxygen species accumulation under salt-stress conditions.
Title: ITN1, a novel gene encoding an ankyrin-repeat protein that affects the ABA-mediated production of reactive oxygen species and is involved in salt-stress tolerance in Arabidopsis thaliana.
molecular, physiological, and functional studies demonstrate that INT1 is a tonoplast-localized H(+)/inositol symporter that mediates the efflux of inositol that is generated during the degradation of inositol-containing compounds in the vacuolar lumen
Title: Functional and physiological characterization of Arabidopsis INOSITOL TRANSPORTER1, a novel tonoplast-localized transporter for myo-inositol.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

MASC_STS16990 (e-PCR)
- UniSTS:258918

NM_180062.3

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables carbohydrate transmembrane transporter activity	ISS Inferred from Sequence or Structural Similarity more info
enables myo-inositol:proton symporter activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within myo-inositol transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transmembrane transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	ISM Inferred from Sequence Model more info
located_in cytosol	HDA more info	PubMed
located_in plant-type vacuole	HDA more info	PubMed
located_in plant-type vacuole membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: inositol transporter 1

NP_850393.1

inositol transporter 1 (INT1); FUNCTIONS IN: carbohydrate transmembrane transporter activity, myo-inositol:hydrogen symporter activity, sugar:hydrogen symporter activity; INVOLVED IN: myo-inositol transport; LOCATED IN: plant-type vacuole membrane, vacuole, membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Sugar transporter, conserved site (InterPro:IPR005829), Major facilitator superfamily (InterPro:IPR020846), General substrate transporter (InterPro:IPR005828), Sugar/inositol transporter (InterPro:IPR003663), Major facilitator superfamily, general substrate transporter (InterPro:IPR016196); BEST Arabidopsis thaliana protein match is: inositol transporter 2 (TAIR:AT1G30220.1); Has 41776 Blast hits to 41190 proteins in 2518 species: Archae - 669; Bacteria - 22523; Metazoa - 5695; Fungi - 8297; Plants - 2788; Viruses - 0; Other Eukaryotes - 1804 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003071.7 Reference assembly

Range

18000822..18004195

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_180062.3 → NP_850393.1 inositol transporter 1 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_850393.1

Status: REVIEWED

UniProtKB/Swiss-Prot

O22848, Q8VZR6

UniProtKB/TrEMBL

A0A178VQH5

Conserved Domains (2) summary

cd06174
Location:35 → 464

MFS; The Major Facilitator Superfamily (MFS) is a large and diverse group of secondary transporters that includes uniporters, symporters, and antiporters. MFS proteins facilitate the transport across cytoplasmic or internal membranes of a variety of ...

pfam00083
Location:34 → 479

Sugar_tr; Sugar (and other) transporter