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Med8 mediator complex subunit 8 [ Mus musculus (house mouse) ]

Gene ID: 80509, updated on 27-Nov-2024

Summary

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Official Symbol
Med8provided by MGI
Official Full Name
mediator complex subunit 8provided by MGI
Primary source
MGI:MGI:1915269
See related
Ensembl:ENSMUSG00000006392 AllianceGenome:MGI:1915269
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
ARC32; 2210021A15Rik
Summary
Predicted to enable RNA polymerase II cis-regulatory region sequence-specific DNA binding activity and transcription coregulator activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be located in nucleus. Predicted to be part of core mediator complex and mediator complex. Is expressed in brain; cerebral cortex; telencephalon ventricular layer; tibialis anterior; and tongue. Orthologous to human MED8 (mediator complex subunit 8). [provided by Alliance of Genome Resources, Nov 2024]
Expression
Ubiquitous expression in CNS E11.5 (RPKM 17.5), liver E14 (RPKM 15.3) and 28 other tissues See more
Orthologs
human all
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Genomic context

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See Med8 in Genome Data Viewer
Location:
4 D2.1; 4 54.61 cM
Exon count:
9
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 4 NC_000070.7 (118266495..118273021)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 4 NC_000070.6 (118409282..118415824)

Chromosome 4 - NC_000070.7Genomic Context describing neighboring genes Neighboring gene protein tyrosine phosphatase receptor type F Neighboring gene predicted gene, 52705 Neighboring gene SZT2 subunit of KICSTOR complex Neighboring gene hydroxypyruvate isomerase (putative) Neighboring gene STARR-positive B cell enhancer ABC_E1281 Neighboring gene STARR-positive B cell enhancer ABC_E6202 Neighboring gene ELOVL fatty acid elongase 1 Neighboring gene cell division cycle 20 Neighboring gene myeloproliferative leukemia virus oncogene

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Szt2, a novel gene for seizure threshold in mice. Frankel WN, et al. Genes Brain Behav, 2009 Jul. PMID 19624305, Free PMC Article
  2. Mediator complex proximal Tail subunit MED30 is critical for Mediator core stability and cardiomyocyte transcriptional network. Tan C, et al. PLoS Genet, 2021 Sep. PMID 34506481, Free PMC Article
  3. Temporal regulation of the Mediator complex during muscle proliferation, differentiation, regeneration, aging, and disease. Kolonay DW, et al. Front Cell Dev Biol, 2024. PMID 38690566, Free PMC Article
  4. Novel critical role of a human Mediator complex for basal RNA polymerase II transcription. Mittler G, et al. EMBO Rep, 2001 Sep. PMID 11559591, Free PMC Article
  5. A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2. Tomomori-Sato C, et al. J Biol Chem, 2004 Feb 13. PMID 14638676

See all (26) citations in PubMed

GeneRIFs: Gene References Into Functions

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Clone Names

  • MNCb-2386

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables transcription coregulator activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables transcription coregulator activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in RNA polymerase II preinitiation complex assembly NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in positive regulation of transcription elongation by RNA polymerase II NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in positive regulation of transcription initiation by RNA polymerase II NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in protein ubiquitination IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of transcription by RNA polymerase II IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
part_of core mediator complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of core mediator complex ISO
Inferred from Sequence Orthology
more info
  
part_of mediator complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of mediator complex ISO
Inferred from Sequence Orthology
more info
 PubMed 
located_in nucleoplasm TAS
Traceable Author Statement
more info
  
located_in nucleus ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
mediator of RNA polymerase II transcription subunit 8
Names
activator-recruited cofactor 32 kDa component
mediator of RNA polymerase II transcription, subunit 8 homolog

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001290688.1 → NP_001277617.1  mediator of RNA polymerase II transcription subunit 8 isoform 3

    See identical proteins and their annotated locations for NP_001277617.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) uses an alternate splice site in te 5' coding region, resulting in initiation of translation at a downstream start site, compared to variant 1. It encodes isoform 3, which is shorter at the N-terminus, compared to isoform 1.
    Source sequence(s)
    AK008759, AK011080, AK148025, BC051683, BY429746
    Consensus CDS
    CCDS71459.1
    UniProtKB/TrEMBL
    Q9CY05
    Related
    ENSMUSP00000081346.5, ENSMUST00000084319.11
    Conserved Domains (1) summary
    pfam10232
    Location:1 → 176
    Med8; Mediator of RNA polymerase II transcription complex subunit 8

  2. NM_020000.3 → NP_064384.2  mediator of RNA polymerase II transcription subunit 8 isoform 1

    See identical proteins and their annotated locations for NP_064384.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
    Source sequence(s)
    AK008759, AK011080, AK148025, BY429746
    Consensus CDS
    CCDS38854.1
    UniProtKB/Swiss-Prot
    Q3UGB4, Q99LM4, Q9D7W5, Q9JJ84
    Related
    ENSMUSP00000019229.9, ENSMUST00000019229.15
    Conserved Domains (2) summary
    pfam09606
    Location:196 → 253
    Med15; ARC105 or Med15 subunit of Mediator complex non-fungal
    pfam10232
    Location:1 → 265
    Med8; Mediator of RNA polymerase II transcription complex subunit 8

  3. NM_173719.3 → NP_776067.1  mediator of RNA polymerase II transcription subunit 8 isoform 2

    See identical proteins and their annotated locations for NP_776067.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) includes an alternate in-frame exon in the 5' coding region and uses an alternate splice site in the 3' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter, compared to isoform 1.
    Source sequence(s)
    AK005422, AK011080, AK148025, BY429746
    Consensus CDS
    CCDS18548.1
    UniProtKB/TrEMBL
    Q9DAY7
    Related
    ENSMUSP00000101992.4, ENSMUST00000106384.10
    Conserved Domains (1) summary
    pfam10232
    Location:1 → 231
    Med8; Mediator of RNA polymerase II transcription complex subunit 8

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000070.7 Reference GRCm39 C57BL/6J

    Range
    118266495..118273021
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006503491.5 → XP_006503554.1  mediator of RNA polymerase II transcription subunit 8 isoform X1

    Conserved Domains (2) summary
    pfam09606
    Location:212 → 269
    Med15; ARC105 or Med15 subunit of Mediator complex non-fungal
    pfam10232
    Location:1 → 281
    Med8; Mediator of RNA polymerase II transcription complex subunit 8

  2. XM_006503492.4 → XP_006503555.1  mediator of RNA polymerase II transcription subunit 8 isoform X2

    See identical proteins and their annotated locations for XP_006503555.1

    UniProtKB/TrEMBL
    Q9CY05
    Conserved Domains (1) summary
    pfam10232
    Location:1 → 176
    Med8; Mediator of RNA polymerase II transcription complex subunit 8

  3. XM_011240659.4 → XP_011238961.1  mediator of RNA polymerase II transcription subunit 8 isoform X2

    See identical proteins and their annotated locations for XP_011238961.1

    UniProtKB/TrEMBL
    Q9CY05
    Conserved Domains (1) summary
    pfam10232
    Location:1 → 176
    Med8; Mediator of RNA polymerase II transcription complex subunit 8
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9D7W5.1 GenPept UniProtKB/Swiss-Prot:Q9D7W5

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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