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Marchf8 membrane associated ring-CH-type finger 8 [ Mus musculus (house mouse) ]

Gene ID: 71779, updated on 27-Nov-2024

Summary

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Official Symbol
Marchf8provided by MGI
Official Full Name
membrane associated ring-CH-type finger 8provided by MGI
Primary source
MGI:MGI:1919029
See related
Ensembl:ENSMUSG00000025702 AllianceGenome:MGI:1919029
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Mir; March8; MARCH-VIII; 1300017E09Rik
Summary
The protein encoded by this gene is a member of the membrane-associated really interesting new gene-CH family of proteins. These proteins are E3 ubiquitin-protein ligases that modulate antigen presentation by downregulating major histocompatibility complex class II surface expression through endocytosis. The transcript is primarily expressed by dendritic cells and macrophages. Overexpression of this gene in antigen presenting cells results in immune defective phenotypes, including resistance to autoimmune disease onset. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
Expression
Ubiquitous expression in kidney adult (RPKM 18.7), liver E14 (RPKM 18.4) and 28 other tissues See more
Orthologs
human all
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Genomic context

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See Marchf8 in Genome Data Viewer
Location:
6 E3; 6 53.78 cM
Exon count:
11
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 6 NC_000072.7 (116312546..116386501)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 6 NC_000072.6 (116337599..116409540)

Chromosome 6 - NC_000072.7Genomic Context describing neighboring genes Neighboring gene WASH complex subunit 2 Neighboring gene predicted gene, 52873 Neighboring gene STARR-positive B cell enhancer ABC_E2799 Neighboring gene zinc finger, AN1-type domain 4 Neighboring gene STARR-seq mESC enhancer starr_17102 Neighboring gene predicted gene, 23527 Neighboring gene predicted gene, 38880 Neighboring gene arachidonate 5-lipoxygenase Neighboring gene olfactory receptor family 13 subfamily A member 1

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Anti-arthritic effect of E3 ubiquitin ligase, c-MIR, expression in the joints. Toyomoto M, et al. Int Immunol, 2011 Mar. PMID 21393633
  2. MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes. Liu X, et al. Nat Commun, 2021 Jul 20. PMID 34285233, Free PMC Article
  3. Ubiquitin ligase MARCH 8 cooperates with CD83 to control surface MHC II expression in thymic epithelium and CD4 T cell selection. Liu H, et al. J Exp Med, 2016 Aug 22. PMID 27503069, Free PMC Article
  4. Thymic CD4 T cell selection requires attenuation of March8-mediated MHCII turnover in cortical epithelial cells through CD83. von Rohrscheidt J, et al. J Exp Med, 2016 Aug 22. PMID 27503071, Free PMC Article
  5. MARCH8 attenuates cGAS-mediated innate immune responses through ubiquitylation. Yang X, et al. Sci Signal, 2022 May 3. PMID 35503863

See all (26) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. E3 Ubiquitin Ligase MARCH8 Promotes Pancreatic Cancer Growth and Metastasis by Activating STAT3 via Degradation of PTPN4.
    Title: E3 Ubiquitin Ligase MARCH8 Promotes Pancreatic Cancer Growth and Metastasis by Activating STAT3 via Degradation of PTPN4.
  2. MARCH8 attenuates cGAS-mediated innate immune responses through ubiquitylation.
    Title: MARCH8 attenuates cGAS-mediated innate immune responses through ubiquitylation.
  3. MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.
    Title: MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes.
  4. CD83-mediated MHCII stabilization through antagonism of March8 as a novel functional adaptation of cortical epithelial cells for T cell selection.
    Title: Thymic CD4 T cell selection requires attenuation of March8-mediated MHCII turnover in cortical epithelial cells through CD83.
  5. orchestrated regulation of MHC II surface expression in thymic epithelial cells by MARCH 8 and CD83 plays a major role in CD4(+) T cell selection.
    Title: Ubiquitin ligase MARCH 8 cooperates with CD83 to control surface MHC II expression in thymic epithelium and CD4 T cell selection.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables MHC class II protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables MHC protein binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ubiquitin protein ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ubiquitin protein ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ubiquitin-protein transferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ubiquitin-protein transferase activity ISO
Inferred from Sequence Orthology
more info
  
enables ubiquitin-protein transferase activity ISS
Inferred from Sequence or Structural Similarity
more info
  
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in adaptive immune response IEA
Inferred from Electronic Annotation
more info
  
involved_in antigen processing and presentation of peptide antigen via MHC class II IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in antigen processing and presentation of peptide antigen via MHC class II IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in immune response IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in negative regulation of MHC class II biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein polyubiquitination IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein polyubiquitination ISO
Inferred from Sequence Orthology
more info
  
involved_in protein ubiquitination IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein ubiquitination IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in Golgi membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in early endosome membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in early endosome membrane IEA
Inferred from Electronic Annotation
more info
  
located_in endoplasmic reticulum membrane IEA
Inferred from Electronic Annotation
more info
  
located_in endosome ISO
Inferred from Sequence Orthology
more info
  
located_in endosome ISS
Inferred from Sequence or Structural Similarity
more info
  
is_active_in late endosome membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in lysosomal membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in lysosome IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in lysosome ISO
Inferred from Sequence Orthology
more info
  
located_in lysosome ISS
Inferred from Sequence or Structural Similarity
more info
  

General protein information

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Preferred Names
E3 ubiquitin-protein ligase MARCHF8
Names
E3 ubiquitin-protein ligase MARCH8
RING-type E3 ubiquitin transferase MARCH8
RING-type E3 ubiquitin transferase MARCHF8
c-mir, cellular modulator of immune recognition
cellular modulator of immune recognition
membrane-associated RING finger protein 8
membrane-associated RING-CH protein VIII
membrane-associated ring finger (C3HC4) 8
NP_001289312.1
NP_001289313.1
NP_001289314.1
NP_001388597.1
NP_001388598.1
NP_001388599.1
NP_001388600.1
NP_001388601.1
NP_001388602.1
NP_082196.1
XP_006506704.1
XP_006506705.1
XP_011239771.1
XP_036008204.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001302383.2 → NP_001289312.1  E3 ubiquitin-protein ligase MARCHF8 isoform 1

    See identical proteins and their annotated locations for NP_001289312.1

    Status: REVIEWED

    Source sequence(s)
    AC123548
    Consensus CDS
    CCDS20451.1
    UniProtKB/Swiss-Prot
    Q9DBD2
    Related
    ENSMUSP00000078024.7, ENSMUST00000079012.13
    Conserved Domains (1) summary
    cd16698
    Location:75 → 126
    RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins

  2. NM_001302384.2 → NP_001289313.1  E3 ubiquitin-protein ligase MARCHF8 isoform 1

    See identical proteins and their annotated locations for NP_001289313.1

    Status: REVIEWED

    Source sequence(s)
    AC123548
    Consensus CDS
    CCDS20451.1
    UniProtKB/Swiss-Prot
    Q9DBD2
    Conserved Domains (1) summary
    cd16698
    Location:75 → 126
    RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins

  3. NM_001302385.2 → NP_001289314.1  E3 ubiquitin-protein ligase MARCHF8 isoform 2

    See identical proteins and their annotated locations for NP_001289314.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) differs in the 5' UTR and lacks an alternate exon in the 5' coding region, which results in use of a downstream start codon compared to variant 1. It encodes isoform 2, which has a shorter N-terminus compared to isoform 1.
    Source sequence(s)
    AC123548
    Consensus CDS
    CCDS85138.1
    UniProtKB/TrEMBL
    A0A0N4SVK0, F6THH9
    Related
    ENSMUSP00000145137.2, ENSMUST00000203193.3
    Conserved Domains (1) summary
    cd16698
    Location:36 → 87
    RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins

  4. NM_001401668.1 → NP_001388597.1  E3 ubiquitin-protein ligase MARCHF8 isoform 3

    Status: REVIEWED

    Source sequence(s)
    AC123548
    UniProtKB/TrEMBL
    A0A0N4SV35
    Related
    ENSMUSP00000144936.2, ENSMUST00000123405.8

  5. NM_001401669.1 → NP_001388598.1  E3 ubiquitin-protein ligase MARCHF8 isoform 4

    Status: REVIEWED

    Source sequence(s)
    AC123548

  6. NM_001401670.1 → NP_001388599.1  E3 ubiquitin-protein ligase MARCHF8 isoform 3

    Status: REVIEWED

    Source sequence(s)
    AC123548
    UniProtKB/TrEMBL
    A0A0N4SV35

  7. NM_001401671.1 → NP_001388600.1  E3 ubiquitin-protein ligase MARCHF8 isoform 5

    Status: REVIEWED

    Source sequence(s)
    AC123548

  8. NM_001401672.1 → NP_001388601.1  E3 ubiquitin-protein ligase MARCHF8 isoform 6

    Status: REVIEWED

    Source sequence(s)
    AC123548
    Related
    ENSMUSP00000115510.3, ENSMUST00000135901.2

  9. NM_001401673.1 → NP_001388602.1  E3 ubiquitin-protein ligase MARCHF8 isoform 2

    Status: REVIEWED

    Source sequence(s)
    AC123548
    UniProtKB/TrEMBL
    A0A0N4SVK0

  10. NM_027920.6 → NP_082196.1  E3 ubiquitin-protein ligase MARCHF8 isoform 1

    See identical proteins and their annotated locations for NP_082196.1

    Status: REVIEWED

    Source sequence(s)
    AC123548
    Consensus CDS
    CCDS20451.1
    UniProtKB/Swiss-Prot
    Q9DBD2
    Related
    ENSMUSP00000098594.4, ENSMUST00000101032.10
    Conserved Domains (1) summary
    cd16698
    Location:75 → 126
    RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000072.7 Reference GRCm39 C57BL/6J

    Range
    116312546..116386501
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011241469.3 → XP_011239771.1  E3 ubiquitin-protein ligase MARCHF8 isoform X3

    See identical proteins and their annotated locations for XP_011239771.1

    Conserved Domains (1) summary
    cd16698
    Location:318 → 369
    RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins

  2. XM_006506641.5 → XP_006506704.1  E3 ubiquitin-protein ligase MARCHF8 isoform X1

    See identical proteins and their annotated locations for XP_006506704.1

    UniProtKB/TrEMBL
    A0A0N4SV35
    Conserved Domains (1) summary
    cd16698
    Location:357 → 408
    RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins

  3. XM_036152311.1 → XP_036008204.1  E3 ubiquitin-protein ligase MARCHF8 isoform X4

    UniProtKB/Swiss-Prot
    Q9DBD2
    Conserved Domains (1) summary
    cd16698
    Location:75 → 126
    RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins

  4. XM_006506642.2 → XP_006506705.1  E3 ubiquitin-protein ligase MARCHF8 isoform X1

    See identical proteins and their annotated locations for XP_006506705.1

    UniProtKB/TrEMBL
    A0A0N4SV35
    Conserved Domains (1) summary
    cd16698
    Location:357 → 408
    RING_CH-C4HC3_MARCH1_like; RING-CH finger, H2 subclass (C4HC3-type), found in membrane-associated RING finger protein MARCH1, MARCH8, and similar proteins
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9DBD2.1 GenPept UniProtKB/Swiss-Prot:Q9DBD2

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