Smurf1 SMAD specific E3 ubiquitin protein ligase 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Smurf1provided by RGD
Official Full Name: SMAD specific E3 ubiquitin protein ligase 1provided by RGD
Primary source: RGD:1594738
See related: EnsemblRapid:ENSRNOG00000000999 AllianceGenome:RGD:1594738
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: RGD1309707
Summary: Predicted to enable SMAD binding activity; signaling receptor binding activity; and ubiquitin protein ligase activity. Involved in positive regulation of axon extension. Located in axon and neuronal cell body. Orthologous to human SMURF1 (SMAD specific E3 ubiquitin protein ligase 1). [provided by Alliance of Genome Resources, Jan 2025]
Expression: Biased expression in Lung (RPKM 81.9), Heart (RPKM 68.5) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 12p11

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	12	NC_086030.1 (14748717..14840273)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	12	NC_051347.1 (9635009..9726520)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	12	NC_005111.4 (11433815..11526465)

Chromosome 12 - NC_086030.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Smurf1 regulates ameloblast polarization by ubiquitination-mediated degradation of RhoA.
Title: Smurf1 regulates ameloblast polarization by ubiquitination-mediated degradation of RhoA.
miR-154-5p Affects the TGFbeta1/Smad3 Pathway on the Fibrosis of Diabetic Kidney Disease via Binding E3 Ubiquitin Ligase Smurf1.
Title: miR-154-5p Affects the TGFβ1/Smad3 Pathway on the Fibrosis of Diabetic Kidney Disease via Binding E3 Ubiquitin Ligase Smurf1.
miR-129-5p restores cardiac function in rats with chronic heart failure by targeting the E3 ubiquitin ligase Smurf1 and promoting PTEN expression.
Title: miR-129-5p restores cardiac function in rats with chronic heart failure by targeting the E3 ubiquitin ligase Smurf1 and promoting PTEN expression.
Smurf1-targeting miR-19b-3p-modified BMSCs combined PLLA composite scaffold to enhance osteogenic activity and treat critical-sized bone defects.
Title: Smurf1-targeting miR-19b-3p-modified BMSCs combined PLLA composite scaffold to enhance osteogenic activity and treat critical-sized bone defects.
microRNA-148a-3p in extracellular vesicles derived from bone marrow mesenchymal stem cells suppresses SMURF1 to prevent osteonecrosis of femoral head.
Title: microRNA-148a-3p in extracellular vesicles derived from bone marrow mesenchymal stem cells suppresses SMURF1 to prevent osteonecrosis of femoral head.
Knockdown of long noncoding RNA XIST mitigates the apoptosis and inflammatory injury of microglia cells after spinal cord injury through miR-27a/Smurf1 axis.
Title: Knockdown of long noncoding RNA XIST mitigates the apoptosis and inflammatory injury of microglia cells after spinal cord injury through miR-27a/Smurf1 axis.
SMURF1 is a key mediator of neuroadaptations in the nucleus accumbens following cocaine exposure and mediates cue-induced cocaine seeking during withdrawal.
Title: E3 Ubiquitin-Protein Ligase SMURF1 in the Nucleus Accumbens Mediates Cocaine Seeking.
Suggest microRNA-424 as a new marker of disease progression in pulmonary arterial hypertension with a role in the development of right ventricular hypertrophy by targeting SMURF1 expression.
Title: MicroRNA-424(322) as a new marker of disease progression in pulmonary arterial hypertension and its role in right ventricular hypertrophy by targeting SMURF1.
that Smurf1 might promote neuronal necroptosis after LPS-induced neuroinflammation
Title: The Role of Smurf1 in Neuronal Necroptosis after Lipopolysaccharide-Induced Neuroinflammation.
Study demonstrated that Smurf1, a negative regulator of osteogenesis, was a real target of miR-503. These findings indicated that miR-503 promoted osteogenesis and accelerated bone formation, which may shed light on the development for a potential therapeutic target for bone repair.
Title: MiR-503 Promotes Bone Formation in Distraction Osteogenesis through Suppressing Smurf1 Expression.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

BE096092 (e-PCR)
- UniSTS:243984

Smurf1 (e-PCR), detects polymorphism
- UniSTS:527557

RH142403 (e-PCR)
- UniSTS:231650

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables I-SMAD binding	ISO Inferred from Sequence Orthology more info
enables R-SMAD binding	ISO Inferred from Sequence Orthology more info
enables SMAD binding	IBA Inferred from Biological aspect of Ancestor more info
enables activin receptor binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transforming growth factor beta receptor binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin-protein transferase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in BMP signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in engulfment of target by autophagosome	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of BMP signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of BMP signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of activin receptor signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of ossification	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transforming growth factor beta receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of axon extension	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of dendrite extension	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in protein export from nucleus	ISO Inferred from Sequence Orthology more info
involved_in protein localization to plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in protein polyubiquitination	ISO Inferred from Sequence Orthology more info
involved_in protein targeting to vacuole involved in autophagy	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein ubiquitination	ISO Inferred from Sequence Orthology more info
involved_in receptor catabolic process	ISO Inferred from Sequence Orthology more info
involved_in regulation of axon extension	ISO Inferred from Sequence Orthology more info
involved_in substrate localization to autophagosome	ISO Inferred from Sequence Orthology more info
involved_in type 2 mitophagy	ISO Inferred from Sequence Orthology more info
involved_in ubiquitin-dependent protein catabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in axon	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase SMURF1

NP_001103068.1

EC 2.3.2.26

XP_006248944.1

EC 2.3.2.26

XP_008767224.1

EC 2.3.2.26

XP_017453951.1

EC 2.3.2.26

XP_038945717.1

EC 2.3.2.26

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001109598.1 → NP_001103068.1 E3 ubiquitin-protein ligase SMURF1

See identical proteins and their annotated locations for NP_001103068.1

Status: PROVISIONAL

Source sequence(s)

CH474012

UniProtKB/TrEMBL

A6K1G6, D3ZCF5

Conserved Domains (3) summary

cd08382
Location:14 → 138

C2_Smurf-like; C2 domain present in Smad ubiquitination-related factor (Smurf)-like proteins

smart00456
Location:281 → 313

WW; Domain with 2 conserved Trp (W) residues

cd00078
Location:373 → 725

HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086030.1 Reference GRCr8

Range

14748717..14840273

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006248882.3 → XP_006248944.1 E3 ubiquitin-protein ligase SMURF1 isoform X4

UniProtKB/TrEMBL

A0A0G2K612, D3ZCF5

Related

ENSRNOP00000073635.2, ENSRNOT00000084577.3

Conserved Domains (4) summary

cd08382
Location:14 → 138

C2_Smurf-like; C2 domain present in Smad ubiquitination-related factor (Smurf)-like proteins

smart00456
Location:307 → 339

WW; Domain with 2 conserved Trp (W) residues

smart00119
Location:423 → 754

HECTc; Domain Homologous to E6-AP Carboxyl Terminus with

cd00078
Location:399 → 754

HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...
XM_017598462.3 → XP_017453951.1 E3 ubiquitin-protein ligase SMURF1 isoform X1

UniProtKB/TrEMBL

D3ZCF5
XM_039089789.2 → XP_038945717.1 E3 ubiquitin-protein ligase SMURF1 isoform X3

UniProtKB/TrEMBL

D3ZCF5

Related

ENSRNOP00000031927.6, ENSRNOT00000031901.8

Conserved Domains (3) summary

cd08382
Location:14 → 138

C2_Smurf-like; C2 domain present in Smad ubiquitination-related factor (Smurf)-like proteins

smart00456
Location:236 → 267

WW; Domain with 2 conserved Trp (W) residues

cd00078
Location:324 → 679

HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...
XM_008769002.3 → XP_008767224.1 E3 ubiquitin-protein ligase SMURF1 isoform X2

UniProtKB/TrEMBL

D3ZCF5

Related

ENSRNOP00000071653.2, ENSRNOT00000082989.3

Conserved Domains (3) summary

cd08382
Location:14 → 138

C2_Smurf-like; C2 domain present in Smad ubiquitination-related factor (Smurf)-like proteins

smart00456
Location:281 → 313

WW; Domain with 2 conserved Trp (W) residues

cd00078
Location:373 → 728

HECTc; HECT domain; C-terminal catalytic domain of a subclass of Ubiquitin-protein ligase (E3). It binds specific ubiquitin-conjugating enzymes (E2), accepts ubiquitin from E2, transfers ubiquitin to substrate lysine side chains, and transfers additional ...