Ankrd2 ankyrin repeat domain 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ankrd2provided by MGI
Official Full Name: ankyrin repeat domain 2provided by MGI
Primary source: MGI:MGI:1861447
See related: Ensembl:ENSMUSG00000025172 AllianceGenome:MGI:1861447
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Arpp
Summary: Enables chromatin binding activity; protein kinase B binding activity; and titin binding activity. Involved in negative regulation of myoblast differentiation. Acts upstream of or within several processes, including negative regulation of myotube differentiation; regulation of intrinsic apoptotic signaling pathway by p53 class mediator; and skeletal muscle cell differentiation. Located in several cellular components, including PML body; euchromatin; and sarcomere. Colocalizes with I band. Is expressed in skeletal musculature; somite; and tongue. Orthologous to human ANKRD2 (ankyrin repeat domain 2). [provided by Alliance of Genome Resources, Jan 2025]
Expression: Biased expression in placenta adult (RPKM 3.1), spleen adult (RPKM 2.4) and 6 other tissues See more
Orthologs: human all
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Genomic context

Location:: 19 C3; 19 35.65 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	19	NC_000085.7 (42024358..42033550)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	19	NC_000085.6 (42035917..42045111)

Chromosome 19 - NC_000085.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

We describe a novel mechanism of signal transduction enriched in medium spiny neurons of striatum that likely mediates effects of the neurotransmitter dopamine acting on these cells. We find that the protein ARPP-16, which is highly expressed in striatal medium spiny neurons, acts as a selective inhibitor of certain forms of the serine/threonine protein phosphatase, PP2A, when phosphorylated by the kinase, MAST3.
Title: ARPP-16 Is a Striatal-Enriched Inhibitor of Protein Phosphatase 2A Regulated by Microtubule-Associated Serine/Threonine Kinase 3 (Mast 3 Kinase).
CARP, Ankrd2, and DARP are not essential for normal cardiac development and function at basal conditions and in response to mechanical pressure overload.
Title: The muscle ankyrin repeat proteins CARP, Ankrd2, and DARP are not essential for normal cardiac development and function at basal conditions and in response to pressure overload.
Ankrd2 as a potent repressor of inflammatory responses through direct interaction with the NF-kappaB repressor subunit p50.
Title: Ankrd2 is a modulator of NF-κB-mediated inflammatory responses during muscle differentiation.
a novel pathway in which SREBP-1/ANKRD2/ID3 activation inhibits myoblast differentiation, and we propose that this pathway acts as a critical determinant of the skeletal muscle developmental program.
Title: Ankyrin repeat domain protein 2 and inhibitor of DNA binding 3 cooperatively inhibit myoblast differentiation by physical interaction.
The Ankrd2 accumulated in the nuclei of myofibers located adjacent to severely damaged myofibers after muscle injury. Ankrd2 may translocate from the I-band to the nucleus in response to muscle damage and in the regulation of gene expression.
Title: Arpp/Ankrd2, a member of the muscle ankyrin repeat proteins (MARPs), translocates from the I-band to the nucleus after muscle injury.
Ankrd2 plays important structural and gene regulatory roles in skeletal muscle.
Title: Structural and regulatory roles of muscle ankyrin repeat protein family in skeletal muscle.
assignment to mouse chromosome 19C3
Title: Assignment of the murine ankyrin-repeated protein gene (Ankrd2) to mouse chromosome 19C3-->D1 and rat chromosome 1q51-->q53 by fluorescence in situ hybridization.
We suggest that Ankrd2, which is also a titin binding protein, is a stretch-response gene associated with slow muscle function and that it is part of a separate mechanotransduction system to the one that regulates muscle mass.
Title: Expression of Ankrd2 in fast and slow muscles and its response to stretch are consistent with a role in slow muscle function.
Ankrd2 gene expression was analyzed at three different stages in differentiating MyoD(-)C2C12 myoblasts
Title: The Ankrd2, Cdkn1c and calcyclin genes are under the control of MyoD during myogenic differentiation.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D19Dcr31 (e-PCR), detects polymorphism
- UniSTS:532351

Ankrd2 (e-PCR), detects polymorphism
- UniSTS:502938

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables RNA polymerase II-specific DNA-binding transcription factor binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II-specific DNA-binding transcription factor binding	ISO Inferred from Sequence Orthology more info
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase B binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase B binding	ISO Inferred from Sequence Orthology more info
enables titin binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of myotube differentiation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of myotube differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of myoblast differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of myoblast differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of myotube differentiation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of myotube differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of cytokine production	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of intrinsic apoptotic signaling pathway by p53 class mediator	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of intrinsic apoptotic signaling pathway by p53 class mediator	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of myoblast proliferation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of myoblast proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to muscle stretch	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within skeletal muscle cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
colocalizes_with I band	IDA Inferred from Direct Assay more info	PubMed
located_in I band	IDA Inferred from Direct Assay more info	PubMed
located_in PML body	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in euchromatin	IDA Inferred from Direct Assay more info	PubMed
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in myofibril	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	NAS Non-traceable Author Statement more info	PubMed
located_in sarcomere	IDA Inferred from Direct Assay more info	PubMed
located_in sarcoplasm	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: ankyrin repeat domain-containing protein 2

Names: ankyrin repeat domain 2 (stretch responsive muscle); skeletal muscle ankyrin repeat protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_020033.2 → NP_064417.2 ankyrin repeat domain-containing protein 2

Status: VALIDATED

Source sequence(s)

AC133503

Consensus CDS

CCDS50437.2

UniProtKB/Swiss-Prot

B2RS77, Q8QZX4, Q9WV06

UniProtKB/TrEMBL

A0A494B9J0

Related

ENSMUSP00000026172.3, ENSMUST00000026172.3

Conserved Domains (2) summary

cd00204
Location:148 → 273

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

sd00045
Location:188 → 217

ANK; ANK repeat [structural motif]

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000085.7 Reference GRCm39 C57BL/6J

Range

42024358..42033550

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017318264.3 → XP_017173753.1 ankyrin repeat domain-containing protein 2 isoform X1

UniProtKB/Swiss-Prot

B2RS77, Q8QZX4, Q9WV06

Conserved Domains (3) summary

sd00045
Location:155 → 184

ANK; ANK repeat [structural motif]

pfam12796
Location:158 → 250

Ank_2; Ankyrin repeats (3 copies)

pfam13637
Location:123 → 174

Ank_4; Ankyrin repeats (many copies)
XM_006527234.4 → XP_006527297.1 ankyrin repeat domain-containing protein 2 isoform X2

UniProtKB/Swiss-Prot

B2RS77, Q8QZX4, Q9WV06

Conserved Domains (3) summary

sd00045
Location:148 → 177

ANK; ANK repeat [structural motif]

pfam12796
Location:118 → 210

Ank_2; Ankyrin repeats (3 copies)

pfam13637
Location:83 → 134

Ank_4; Ankyrin repeats (many copies)