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IWS1 interacts with SUPT6H, CTD assembly factor 1 [ Homo sapiens (human) ]

Gene ID: 55677, updated on 27-Nov-2024

Summary

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Official Symbol
IWS1provided by HGNC
Official Full Name
interacts with SUPT6H, CTD assembly factor 1provided by HGNC
Primary source
HGNC:HGNC:25467
See related
Ensembl:ENSG00000163166 AllianceGenome:HGNC:25467
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Summary
Involved in regulation of mRNA export from nucleus; regulation of mRNA processing; and transcription elongation-coupled chromatin remodeling. Located in nucleoplasm. [provided by Alliance of Genome Resources, Nov 2024]
Expression
Ubiquitous expression in testis (RPKM 10.7), lymph node (RPKM 10.6) and 25 other tissues See more
Orthologs
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Genomic context

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See IWS1 in Genome Data Viewer
Location:
2q14.3
Exon count:
16
Annotation release Status Assembly Chr Location
RS_2024_08 current GRCh38.p14 (GCF_000001405.40) 2 NC_000002.12 (127480812..127527336, complement)
RS_2024_08 current T2T-CHM13v2.0 (GCF_009914755.1) 2 NC_060926.1 (127916064..127962573, complement)
RS_2024_09 previous assembly GRCh37.p13 (GCF_000001405.25) 2 NC_000002.11 (128238388..128284065, complement)

Chromosome 2 - NC_000002.12Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC124907884 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:128221175-128221782 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:128237383-128238334 Neighboring gene RNA, U4 small nuclear 48, pseudogene Neighboring gene H3K4me1 hESC enhancer GRCh37_chr2:128272241-128272823 Neighboring gene NANOG-H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:128283275-128283974 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16491 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16492 Neighboring gene ReSE screen-validated silencer GRCh37_chr2:128284386-128284559 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16493 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr2:128293291-128294141 Neighboring gene H3K27ac hESC enhancer GRCh37_chr2:128303085-128303585 Neighboring gene myosin VIIB Neighboring gene uncharacterized LOC105373609 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16494 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16495 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr2:128335179-128335732 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16496 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 16497 Neighboring gene uncharacterized LOC101927834

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. A putative transcriptional elongation factor hIws1 is essential for mammalian cell proliferation. Liu Z, et al. Biochem Biophys Res Commun, 2007 Feb 2. PMID 17184735
  2. A sequence motif conserved in diverse nuclear proteins identifies a protein interaction domain utilised for nuclear targeting by human TFIIS. Ling Y, et al. Nucleic Acids Res, 2006. PMID 16648364, Free PMC Article
  3. A ubiquitous disordered protein interaction module orchestrates transcription elongation. Cermakova K, et al. Science, 2021 Nov 26. PMID 34822292, Free PMC Article
  4. The integrase cofactor LEDGF/p75 associates with Iws1 and Spt6 for postintegration silencing of HIV-1 gene expression in latently infected cells. Gérard A, et al. Cell Host Microbe, 2015 Jan 14. PMID 25590759
  5. HDGF-related protein-2 (HRP-2) acts as an oncogene to promote cell growth in hepatocellular carcinoma. Gao K, et al. Biochem Biophys Res Commun, 2015 Mar 20. PMID 25689719

See all (70) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. A ubiquitous disordered protein interaction module orchestrates transcription elongation.
    Title: A ubiquitous disordered protein interaction module orchestrates transcription elongation.
  2. common LEDGF/p75 interaction interface shared by JPO2, PogZ, MLL1, IWS1 and HIV IN
    Title: Multiple cellular proteins interact with LEDGF/p75 through a conserved unstructured consensus motif.
  3. Altogether, these results indicate that a complex containing LEDGF/p75, Iws1, and Spt6 participates in regulating postintegration steps of HIV latency.
    Title: The integrase cofactor LEDGF/p75 associates with Iws1 and Spt6 for postintegration silencing of HIV-1 gene expression in latently infected cells.
  4. IWS1, an RNA processing regulator, is phosphorylated by Akt3 and Akt1 at Ser720/Thr721 in lung cancer
    Title: Phosphoproteomics screen reveals akt isoform-specific signals linking RNA processing to lung cancer.
  5. recombinant Spt6 binds selectively to a stretch of uninterrupted consensus repeats located in the N-terminal half of the CTD and recruits Iws1
    Title: The Iws1:Spt6:CTD complex controls cotranscriptional mRNA biosynthesis and HYPB/Setd2-mediated histone H3K36 methylation.
  6. binding of Spt6 to Ser2-P RNAPII provides a cotranscriptional mechanism to recruit Iws1, REF1/Aly, and associated mRNA processing, surveillance, and export factors to responsive genes.
    Title: The Spt6 SH2 domain binds Ser2-P RNAPII to direct Iws1-dependent mRNA splicing and export.
  7. Here we report that the human homolog of Iws1, hIws1, physically interacts with protein arginine methyltransferases PRMT5 which methylates elongation factor Spt5 and regulates its interaction with RNA polymerase II.
    Title: A putative transcriptional elongation factor hIws1 is essential for mammalian cell proliferation.
Submit: New GeneRIF Correction

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Common genetic variants associated with cognitive performance identified using the proxy-phenotype method.
EBI GWAS Catalog

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Replication interactions

Interaction Pubs
Knockdown of IWS1 by shRNA enhances HIV-1 replication in HIV-1-infected CD4+ T-cells and latently infected cells PubMed

Protein interactions

Protein Gene Interaction Pubs
integrase gag-pol The IN catalytic core domain has a higher binding affinity (Kd) for PSIP1 (LEDGF/p75) than the physiological binding partner, IWS1 PubMed
gag-pol HIV-1 IN displaces IWS1 from PSIP1 (LEDGF/p75) efficiently PubMed
gag-pol The HIV-1 IN interaction site on the PSIP1 (LEDGF/p75) integrase binding domain (IBD) overlaps with that of IWS1 PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • FLJ10006, FLJ14655, FLJ32319, MGC126375, MGC126376, DKFZp761G0123

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in RNA splicing IEA
Inferred from Electronic Annotation
more info
  
involved_in chromatin remodeling IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in poly(A)+ mRNA export from nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of mRNA export from nucleus IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of mRNA processing IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in transcription elongation-coupled chromatin remodeling IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
  
located_in nucleoplasm TAS
Traceable Author Statement
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
protein IWS1 homolog
Names
IWS1 homolog
IWS1, SUPT6H interacting protein
IWS1-like protein
interacts with Spt6

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001410923.1 → NP_001397852.1  protein IWS1 homolog isoform 2

    Status: VALIDATED

    Source sequence(s)
    AC010976
    Consensus CDS
    CCDS92859.1
    UniProtKB/TrEMBL
    A0A1B0GW95
    Related
    ENSP00000490836.2, ENST00000637187.2

  2. NM_017969.3 → NP_060439.2  protein IWS1 homolog isoform 1

    See identical proteins and their annotated locations for NP_060439.2

    Status: VALIDATED

    Source sequence(s)
    AI638093, BC110537, DA268882
    Consensus CDS
    CCDS2146.1
    UniProtKB/Swiss-Prot
    Q2TB65, Q6P157, Q8N3E8, Q96MI7, Q96ST2, Q9NV97, Q9NWH8
    UniProtKB/TrEMBL
    B4DL52
    Related
    ENSP00000295321.4, ENST00000295321.9
    Conserved Domains (2) summary
    pfam08618
    Location:228 → 332
    Opi1; Transcription factor Opi1
    cl00146
    Location:528 → 792
    TFIIS_I; N-terminal domain (domain I) of transcription elongation factor S-II (TFIIS); similar to a domain found in elongin A and CRSP70; likely to be involved in transcription; domain I from TFIIS interacts with RNA polymerase II holoenzyme

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2024_08

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000002.12 Reference GRCh38.p14 Primary Assembly

    Range
    127480812..127527336 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_005263707.4 → XP_005263764.1  protein IWS1 homolog isoform X1

    UniProtKB/TrEMBL
    B4DL52
    Conserved Domains (2) summary
    pfam08618
    Location:233 → 337
    Opi1; Transcription factor Opi1
    cl00146
    Location:533 → 797
    TFIIS_I; N-terminal domain (domain I) of transcription elongation factor S-II (TFIIS); similar to a domain found in elongin A and CRSP70; likely to be involved in transcription; domain I from TFIIS interacts with RNA polymerase II holoenzyme

  2. XM_006712626.4 → XP_006712689.1  protein IWS1 homolog isoform X2

    UniProtKB/TrEMBL
    B4DL52
    Conserved Domains (2) summary
    pfam08618
    Location:233 → 337
    Opi1; Transcription factor Opi1
    cl00146
    Location:533 → 782
    TFIIS_I; N-terminal domain (domain I) of transcription elongation factor S-II (TFIIS); similar to a domain found in elongin A and CRSP70; likely to be involved in transcription; domain I from TFIIS interacts with RNA polymerase II holoenzyme

  3. XM_017004468.3 → XP_016859957.1  protein IWS1 homolog isoform X4

    Conserved Domains (2) summary
    pfam08618
    Location:21 → 125
    Opi1; Transcription factor Opi1
    cl00146
    Location:221 → 585
    TFIIS_I; N-terminal domain (domain I) of transcription elongation factor S-II (TFIIS); similar to a domain found in elongin A and CRSP70; likely to be involved in transcription; domain I from TFIIS interacts with RNA polymerase II holoenzyme

  4. XM_047444948.1 → XP_047300904.1  protein IWS1 homolog isoform X3

  5. XM_011511450.2 → XP_011509752.1  protein IWS1 homolog isoform X3

    UniProtKB/TrEMBL
    B4DL52
    Conserved Domains (2) summary
    pfam08618
    Location:233 → 337
    Opi1; Transcription factor Opi1
    cl00146
    Location:533 → 724
    TFIIS_I; N-terminal domain (domain I) of transcription elongation factor S-II (TFIIS); similar to a domain found in elongin A and CRSP70; likely to be involved in transcription; domain I from TFIIS interacts with RNA polymerase II holoenzyme

  6. XM_047444951.1 → XP_047300907.1  protein IWS1 homolog isoform X5

RNA

  1. XR_001738828.2 RNA Sequence

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060926.1 Alternate T2T-CHM13v2.0

    Range
    127916064..127962573 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054342889.1 → XP_054198864.1  protein IWS1 homolog isoform X1

  2. XM_054342890.1 → XP_054198865.1  protein IWS1 homolog isoform X2

  3. XM_054342891.1 → XP_054198866.1  protein IWS1 homolog isoform X3

  4. XM_054342892.1 → XP_054198867.1  protein IWS1 homolog isoform X3

  5. XM_054342893.1 → XP_054198868.1  protein IWS1 homolog isoform X5

RNA

  1. XR_008486446.1 RNA Sequence

Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q96ST2.2 GenPept UniProtKB/Swiss-Prot:Q96ST2

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