Crk CRK proto-oncogene, adaptor protein [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Crkprovided by RGD
Official Full Name: CRK proto-oncogene, adaptor proteinprovided by RGD
Primary source: RGD:2405
See related: EnsemblRapid:ENSRNOG00000025792 AllianceGenome:RGD:2405
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Crko; CrkII
Summary: Enables several functions, including insulin-like growth factor receptor binding activity; protein tyrosine kinase binding activity; and scaffold protein binding activity. Involved in several processes, including cellular response to nitric oxide; cellular response to peptide hormone stimulus; and regulation of intracellular signal transduction. Predicted to be located in actin cytoskeleton and membrane. Predicted to be part of protein-containing complex. Predicted to be active in cytoplasm and neuromuscular junction. Biomarker of degenerative disc disease. Orthologous to human CRK (CRK proto-oncogene, adaptor protein). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Heart (RPKM 496.7), Muscle (RPKM 438.6) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 10q24

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	10	NC_086028.1 (61028736..61054971)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	10	NC_051345.1 (60530469..60556703)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	10	NC_005109.4 (63829731..63855999)

Chromosome 10 - NC_086028.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

overexpression of miR-126 significantly inhibited the activation and migration of hepatic stellate cell through targeting CRK.
Title: Overexpression of miR-126 inhibits the activation and migration of HSCs through targeting CRK.
study provides evidence that Crk phosphorylation induced specifically by engagement of immunoreceptor tyrosine-based inhibition motif-containing receptors contributes to inhibition of NK cells
Title: Inhibitory receptor signaling via tyrosine phosphorylation of the adaptor Crk.
ROS affect the key adapter protein CrkII signaling by two ways: stimulation of CkII phosphorylation and a functional consequence: formation of CrkII-protein complexes.
Title: Adapter protein CRKII signaling is involved in the rat pancreatic acini response to reactive oxygen species.
Crk may be involved in regulation of cell motility by a hyaluronic acid-dependent mechanism through an association with ERMs
Title: Crk associates with ERM proteins and promotes cell motility toward hyaluronic acid.
CCK-induced CrkII electrophoretic shift was dependent on the Src family of tyrosine kinases and occurred in the intact animal, suggesting a physiological role of CrkII mediating CCK actions in the exocrine pancreas in vivo
Title: Cholecystokinin rapidly stimulates CrkII function in vivo in rat pancreatic acini. Formation of CrkII-protein complexes.
The Y221 site in transfected rat CrkII regulates Rac membrane translocation upon cell adhesion, which is necessary for activation of downstream Rac signaling pathways.
Title: Tyrosine 221 in Crk regulates adhesion-dependent membrane localization of Crk and Rac and activation of Rac signaling.
nitric oxide induced dissociation of the Cas-Crk complex was mimicked by overexpression of PTP-PEST in vascular smooth muscle
Title: Nitric oxide-induced inhibition of aortic smooth muscle cell motility: role of PTP-PEST and adaptor proteins p130cas and Crk.
Rac1 activation by paxillin-Crk-DOCK180 signaling complex is antagonized by Rap1 in migrating rat cells
Title: Activation of Rac1 by paxillin-Crk-DOCK180 signaling complex is antagonized by Rap1 in migrating NBT-II cells.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH94394 (e-PCR)
- UniSTS:119642

CRK (e-PCR)
- UniSTS:266693

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables SH2 domain binding	ISO Inferred from Sequence Orthology more info
enables SH3 domain binding	ISO Inferred from Sequence Orthology more info
enables cytoskeletal protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables enzyme binding	IPI Inferred from Physical Interaction more info	PubMed
enables ephrin receptor binding	ISO Inferred from Sequence Orthology more info
enables insulin-like growth factor receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables kinase binding	ISO Inferred from Sequence Orthology more info
enables phosphotyrosine residue binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein phosphorylated amino acid binding	ISO Inferred from Sequence Orthology more info
enables protein tyrosine kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein tyrosine kinase binding	ISO Inferred from Sequence Orthology more info
enables protein-macromolecule adaptor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables receptor tyrosine kinase binding	IBA Inferred from Biological aspect of Ancestor more info
enables scaffold protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables signaling adaptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables signaling adaptor activity	ISO Inferred from Sequence Orthology more info
enables signaling receptor complex adaptor activity	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in actin cytoskeleton organization	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within activation of GTPase activity	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cell chemotaxis	ISO Inferred from Sequence Orthology more info
involved_in cell migration	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in cellular response to endothelin	IPI Inferred from Physical Interaction more info	PubMed
involved_in cellular response to insulin-like growth factor stimulus	IPI Inferred from Physical Interaction more info	PubMed
involved_in cellular response to nerve growth factor stimulus	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to nitric oxide	IPI Inferred from Physical Interaction more info	PubMed
involved_in cellular response to transforming growth factor beta stimulus	IPI Inferred from Physical Interaction more info	PubMed
acts_upstream_of_or_within cerebellar neuron development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cerebral cortex development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within dendrite development	ISO Inferred from Sequence Orthology more info
involved_in enzyme-linked receptor protein signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in enzyme-linked receptor protein signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in ephrin receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in ephrin receptor signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within establishment of cell polarity	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within helper T cell diapedesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within hippocampus development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within lipid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cell motility	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of natural killer cell mediated cytotoxicity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of wound healing	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within neuron migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of JNK cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of Rac protein signal transduction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell growth	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of intracellular signal transduction	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of skeletal muscle acetylcholine-gated channel clustering	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of smooth muscle cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of substrate adhesion-dependent cell spreading	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of substrate adhesion-dependent cell spreading	ISS Inferred from Sequence or Structural Similarity more info
involved_in postsynaptic specialization assembly	ISO Inferred from Sequence Orthology more info
involved_in protein localization to membrane	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within reelin-mediated signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in regulation of Rac protein signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of T cell migration	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in regulation of actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in regulation of actin cytoskeleton organization	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within regulation of cell adhesion mediated by integrin	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell motility	IEA Inferred from Electronic Annotation more info
NOT involved_in regulation of cell motility	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell shape	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell shape	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within regulation of dendrite development	ISO Inferred from Sequence Orthology more info
involved_in regulation of intracellular signal transduction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of leukocyte migration	ISO Inferred from Sequence Orthology more info
involved_in regulation of signal transduction	ISO Inferred from Sequence Orthology more info
NOT involved_in regulation of wound healing	ISO Inferred from Sequence Orthology more info
involved_in response to cholecystokinin	IDA Inferred from Direct Assay more info	PubMed
involved_in response to hepatocyte growth factor	IPI Inferred from Physical Interaction more info	PubMed
involved_in response to hydrogen peroxide	IPI Inferred from Physical Interaction more info	PubMed
involved_in response to peptide	IPI Inferred from Physical Interaction more info	PubMed
involved_in response to yeast	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
located_in actin cytoskeleton	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	TAS Traceable Author Statement more info
located_in membrane	ISO Inferred from Sequence Orthology more info
is_active_in neuromuscular junction	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: adapter molecule crk

Names: avian sarcoma virus CT10 (v-crk) oncogene homolog; p38; proto-oncogene C-crk; v-crk avian sarcoma virus CT10 oncogene homolog; v-crk sarcoma virus CT10 oncogene homolog

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_019302.1 → NP_062175.1 adapter molecule crk

See identical proteins and their annotated locations for NP_062175.1

Status: PROVISIONAL

Source sequence(s)

D44481

UniProtKB/Swiss-Prot

Q63768

UniProtKB/TrEMBL

A0A8I6ANJ0, A6HGT2

Related

ENSRNOP00000106726.1, ENSRNOT00000133236.1

Conserved Domains (3) summary

cd09926
Location:5 → 122

SH2_CRK_like; Src homology 2 domain found in cancer-related signaling adaptor protein CRK

cd11758
Location:135 → 189

SH3_CRK_N; N-terminal Src Homology 3 domain of Ct10 Regulator of Kinase adaptor proteins

cd11759
Location:237 → 293

SH3_CRK_C; C-terminal Src Homology 3 domain of Ct10 Regulator of Kinase adaptor proteins

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086028.1 Reference GRCr8

Range

61028736..61054971

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006246913.3 → XP_006246975.1 adapter molecule crk isoform X1

See identical proteins and their annotated locations for XP_006246975.1

Conserved Domains (2) summary

cd09926
Location:5 → 122

SH2_CRK_like; Src homology 2 domain found in cancer-related signaling adaptor protein CRK

cd11758
Location:135 → 189

SH3_CRK_N; N-terminal Src Homology 3 domain of Ct10 Regulator of Kinase adaptor proteins