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sif still life [ Drosophila melanogaster (fruit fly) ]

Gene ID: 43892, updated on 9-Dec-2024

Summary

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Official Symbol
sifprovided by FlyBase
Official Full Name
still lifeprovided by FlyBase
Primary source
FLYBASE:FBgn0085447
Locus tag
Dmel_CG34418
See related
AllianceGenome:FB:FBgn0085447
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Drosophila melanogaster
Lineage
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as
CG32414; CG34418; CG5256; CG5406; Dmel\CG34418; Dm_3L:26493; lincRNA.447; RhoGEF; Sif; SIF; slf; Stilllife
Old locus tag
CG32414; CG5406; Dmel_CG32414; Dmel_CG5406
Summary
Predicted to enable guanyl-nucleotide exchange factor activity. Involved in several processes, including actin cytoskeleton organization; regulation of plasma membrane bounded cell projection organization; and visual perception. Located in synapse. Is expressed in several structures, including ganglia; organism subdivision; presumptive embryonic/larval central nervous system; spermatozoon; and synaptic neuropil domain. Used to study cataract 48 and neurodevelopmental disorder with language delay and seizures. Human ortholog(s) of this gene implicated in neurodevelopmental disorder with language delay and seizures. Orthologous to human TIAM1 (TIAM Rac1 associated GEF 1) and TIAM2 (TIAM Rac1 associated GEF 2). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs
all
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Genomic context

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See sif in Genome Data Viewer
Location:
64E1-64E5; 3-15 cM
Exon count:
44
Annotation release Status Assembly Chr Location
Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 3L NT_037436.4 (5657913..5749599)
Release 5.57 previous assembly Release 5 (GCF_000001215.2) 3L NT_037436.3 (5651013..5742699)

Chromosome 3L - NT_037436.4Genomic Context describing neighboring genes Neighboring gene Blimp-1 Neighboring gene lin-28 Neighboring gene uncharacterized protein Neighboring gene Separase Neighboring gene uncharacterized protein Neighboring gene DnaJ-like-1 Neighboring gene Ubiquitin specific protease 47

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Still life, a protein in synaptic terminals of Drosophila homologous to GDP-GTP exchangers. Sone M. Science, 1997 Mar 7. PMID 9072802
  2. Loss-of-function variants in TIAM1 are associated with developmental delay, intellectual disability, and seizures. Lu S, et al. Am J Hum Genet, 2022 Apr 7. PMID 35240055, Free PMC Article
  3. EVH1/WH1 domains of VASP and WASP proteins belong to a large family including Ran-binding domains of the RanBP1 family. Callebaut I, et al. FEBS Lett, 1998 Dec 18. PMID 9883880
  4. Bi-allelic Loss-of-Function Variants in DNMBP Cause Infantile Cataracts. Ansar M, et al. Am J Hum Genet, 2018 Oct 4. PMID 30290152, Free PMC Article
  5. Coordinating synaptic growth without being a nervous wreck. Collins CA, et al. Neuron, 2004 Feb 19. PMID 14980197

See all (90) citations in PubMed

GeneRIFs: Gene References Into Functions

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by FlyBase

Function Evidence Code Pubs
enables guanyl-nucleotide exchange factor activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables guanyl-nucleotide exchange factor activity IEA
Inferred from Electronic Annotation
more info
  
enables guanyl-nucleotide exchange factor activity ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
Process Evidence Code Pubs
involved_in actin cytoskeleton organization IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in activation of GTPase activity IEA
Inferred from Electronic Annotation
more info
  
involved_in eye development IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of axonogenesis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in positive regulation of filopodium assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of axonogenesis IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in regulation of axonogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of synapse structure or activity TAS
Traceable Author Statement
more info
 PubMed 
involved_in small GTPase-mediated signal transduction IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in small GTPase-mediated signal transduction IEA
Inferred from Electronic Annotation
more info
  
involved_in visual perception IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in synapse IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in synapse IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
still life
Names
CG34418-PA
CG34418-PB
CG34418-PC
CG34418-PF
CG34418-PI
CG34418-PJ
CG34418-PK
CG34418-PL
CG34418-PM
CG34418-PN
CG34418-PO
CG34418-PP
CG34418-PQ
CG34418-PR
sif-PA
sif-PB
sif-PC
sif-PF
sif-PI
sif-PJ
sif-PK
sif-PL
sif-PM
sif-PN
sif-PO
sif-PP
sif-PQ
sif-PR
still life type 1
still life type 2
still-life

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NT_037436.4 Reference assembly

    Range
    5657913..5749599
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001104048.2 → NP_001097518.2  still life, isoform N [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001097518.2

    Status: REVIEWED

    UniProtKB/TrEMBL
    A8JNL4
    Conserved Domains (7) summary
    cd01818
    Location:1122 → 1200
    TIAM1_RBD; Ubiquitin domain of Tiam1 guanine nucleotide exchange factor
    smart00461
    Location:35 → 144
    WH1; WASP homology region 1
    cd00160
    Location:1446 → 1637
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd00992
    Location:1202 → 1289
    PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
    cd01230
    Location:841 → 966
    PH1_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, N-terminal domain
    cd01255
    Location:1640 → 1813
    PH2_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, C-terminal domain
    pfam00169
    Location:854 → 955
    PH; PH domain

  2. NM_001104049.4 → NP_001097519.2  still life, isoform I [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001097519.2

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    P91621, Q9VRN7
    UniProtKB/TrEMBL
    A8JNL6
    Conserved Domains (7) summary
    cd01818
    Location:1122 → 1200
    TIAM1_RBD; Ubiquitin domain of Tiam1 guanine nucleotide exchange factor
    smart00461
    Location:35 → 144
    WH1; WASP homology region 1
    cd00160
    Location:1437 → 1628
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd00992
    Location:1202 → 1289
    PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
    cd01230
    Location:841 → 966
    PH1_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, N-terminal domain
    cd01255
    Location:1631 → 1804
    PH2_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, C-terminal domain
    pfam00169
    Location:854 → 955
    PH; PH domain

  3. NM_001347763.1 → NP_001334674.1  still life, isoform O [Drosophila melanogaster]

    Status: REVIEWED

    UniProtKB/TrEMBL
    X2JC30

  4. NM_001347764.1 → NP_001334675.1  still life, isoform Q [Drosophila melanogaster]

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A0S0WGZ0

  5. NM_001274519.3 → NP_001261448.1  still life, isoform M [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001261448.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    M9PE98
    Conserved Domains (7) summary
    cd01818
    Location:1132 → 1210
    TIAM1_RBD; Ubiquitin domain of Tiam1 guanine nucleotide exchange factor
    smart00461
    Location:35 → 140
    WH1; WASP homology region 1
    cd00160
    Location:1456 → 1647
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd00992
    Location:1212 → 1299
    PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
    cd01230
    Location:851 → 976
    PH1_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, N-terminal domain
    cd01255
    Location:1650 → 1823
    PH2_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, C-terminal domain
    pfam00169
    Location:864 → 965
    PH; PH domain

  6. NM_001300018.2 → NP_001286947.1  still life, isoform P [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001286947.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    X2JCJ4
    Conserved Domains (6) summary
    cd01818
    Location:932 → 1010
    TIAM1_RBD; Ubiquitin domain of Tiam1 guanine nucleotide exchange factor
    cd00160
    Location:1256 → 1447
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd00992
    Location:1012 → 1099
    PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
    cd01230
    Location:651 → 776
    PH1_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, N-terminal domain
    cd01255
    Location:1450 → 1623
    PH2_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, C-terminal domain
    pfam00169
    Location:664 → 765
    PH; PH domain

  7. NM_001274517.3 → NP_001261446.1  still life, isoform J [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001261446.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    M9PHI5
    Conserved Domains (7) summary
    cd01818
    Location:1141 → 1219
    TIAM1_RBD; Ubiquitin domain of Tiam1 guanine nucleotide exchange factor
    smart00461
    Location:35 → 144
    WH1; WASP homology region 1
    cd00160
    Location:1456 → 1647
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd00992
    Location:1221 → 1308
    PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
    cd01230
    Location:860 → 985
    PH1_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, N-terminal domain
    cd01255
    Location:1650 → 1823
    PH2_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, C-terminal domain
    pfam00169
    Location:873 → 974
    PH; PH domain

  8. NM_001104047.5 → NP_001097517.1  still life, isoform F [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001097517.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    Q8IQ62
    Conserved Domains (7) summary
    cd01818
    Location:1122 → 1200
    TIAM1_RBD; Ubiquitin domain of Tiam1 guanine nucleotide exchange factor
    smart00461
    Location:35 → 144
    WH1; WASP homology region 1
    cd00160
    Location:1437 → 1628
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd00992
    Location:1202 → 1289
    PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
    cd01230
    Location:841 → 966
    PH1_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, N-terminal domain
    cd01255
    Location:1631 → 1804
    PH2_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, C-terminal domain
    pfam00169
    Location:854 → 955
    PH; PH domain

  9. NM_168126.4 → NP_729084.1  still life, isoform B [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_729084.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    P91621, Q9VRN7
    UniProtKB/TrEMBL
    A8JNL6
    Related
    FBpp0111620
    Conserved Domains (7) summary
    cd01818
    Location:1122 → 1200
    TIAM1_RBD; Ubiquitin domain of Tiam1 guanine nucleotide exchange factor
    smart00461
    Location:35 → 144
    WH1; WASP homology region 1
    cd00160
    Location:1437 → 1628
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd00992
    Location:1202 → 1289
    PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
    cd01230
    Location:841 → 966
    PH1_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, N-terminal domain
    cd01255
    Location:1631 → 1804
    PH2_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, C-terminal domain
    pfam00169
    Location:854 → 955
    PH; PH domain

  10. NM_001414089.1 → NP_001401030.1  still life, isoform R [Drosophila melanogaster]

    Status: REVIEWED

    UniProtKB/TrEMBL
    M9PBR2

  11. NM_001274518.2 → NP_001261447.1  still life, isoform L [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001261447.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    M9PBR2
    Conserved Domains (6) summary
    cd00160
    Location:642 → 833
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd00992
    Location:398 → 485
    PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
    cd01230
    Location:37 → 162
    PH1_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, N-terminal domain
    cd01255
    Location:836 → 1009
    PH2_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, C-terminal domain
    pfam00169
    Location:50 → 151
    PH; PH domain
    cl00155
    Location:318 → 396
    UBQ; Ubiquitin-like proteins

  12. NM_206278.2 → NP_996000.1  still life, isoform C [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_996000.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    P91620, Q8MT93, Q9VRN8
    Conserved Domains (6) summary
    cd00160
    Location:1426 → 1617
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd00992
    Location:1182 → 1269
    PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
    cd01230
    Location:821 → 946
    PH1_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, N-terminal domain
    cd01255
    Location:1620 → 1793
    PH2_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, C-terminal domain
    pfam00169
    Location:834 → 935
    PH; PH domain
    cl00155
    Location:1102 → 1180
    UBQ; Ubiquitin-like proteins

  13. NM_079908.3 → NP_524647.2  still life, isoform A [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_524647.2

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    P91620
    Related
    FBpp0111621
    Conserved Domains (6) summary
    cd00160
    Location:1417 → 1608
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd00992
    Location:1182 → 1269
    PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
    cd01230
    Location:821 → 946
    PH1_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, N-terminal domain
    cd01255
    Location:1611 → 1784
    PH2_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, C-terminal domain
    pfam00169
    Location:834 → 935
    PH; PH domain
    cl00155
    Location:1102 → 1180
    UBQ; Ubiquitin-like proteins

  14. NM_001104046.4 → NP_001097516.3  still life, isoform K [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001097516.3

    Status: REVIEWED

    UniProtKB/TrEMBL
    A8JNL5
    Conserved Domains (6) summary
    cd01818
    Location:1102 → 1180
    TIAM1_RBD; Ubiquitin domain of Tiam1 guanine nucleotide exchange factor
    cd00160
    Location:1426 → 1617
    RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.
    cd00992
    Location:1182 → 1269
    PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
    cd01230
    Location:821 → 946
    PH1_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, N-terminal domain
    cd01255
    Location:1620 → 1793
    PH2_Tiam1_2; T-lymphoma invasion and metastasis 1 and 2 Pleckstrin Homology (PH) domain, C-terminal domain
    pfam00169
    Location:834 → 935
    PH; PH domain
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
A0A0S0WGZ0 A0A0S0WGZ0
A8JNL4 A8JNL4
A8JNL5 A8JNL5
A8JNL6 A8JNL6
M9PBR2 M9PBR2
M9PE98 M9PE98
M9PHI5 M9PHI5
P91620.2 P91620
P91621.2 P91621
Q8IQ62 Q8IQ62
X2JC30 X2JC30
X2JCJ4 X2JCJ4

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