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puf puffyeye [ Drosophila melanogaster (fruit fly) ]

Gene ID: 42935, updated on 9-Dec-2024

Summary

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Official Symbol
pufprovided by FlyBase
Official Full Name
puffyeyeprovided by FlyBase
Primary source
FLYBASE:FBgn0039214
Locus tag
Dmel_CG5794
See related
AllianceGenome:FB:FBgn0039214
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Drosophila melanogaster
Lineage
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as
BcDNA:AT28817; CG5794; Dmel\CG5794; NEST:bs19d10; Puf; Q9VC56; Usp34; USP34
Summary
Predicted to enable cysteine-type deubiquitinase activity. Involved in several processes, including negative regulation of Toll signaling pathway; negative regulation of defense response; and positive regulation of double-strand break repair via homologous recombination. Located in nucleus. Is expressed in adult head and spermatozoon. Orthologous to human USP34 (ubiquitin specific peptidase 34). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs
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Genomic context

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See puf in Genome Data Viewer
Location:
96A13-96A13; 3-85 cM
Exon count:
20
Annotation release Status Assembly Chr Location
Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 3R NT_033777.3 (24635270..24650043, complement)
Release 5.57 previous assembly Release 5 (GCF_000001215.2) 3R NT_033777.2 (20460992..20475765, complement)

Chromosome 3R - NT_033777.3Genomic Context describing neighboring genes Neighboring gene atlastin Neighboring gene wsck Neighboring gene Metallothionein F Neighboring gene absent, small, or homeotic discs 2 Neighboring gene uncharacterized protein Neighboring gene uncharacterized protein Neighboring gene mir-1003 stem loop

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The ubiquitin thioesterase YOD1 ameliorates mutant Huntingtin induced pathology in Drosophila. Farkas A, et al. Sci Rep, 2023 Dec 11. PMID 38081944, Free PMC Article
  2. The Drosophila ubiquitin-specific protease Puffyeye regulates dMyc-mediated growth. Li L, et al. Development, 2013 Dec. PMID 24173801, Free PMC Article
  3. Model system identification of novel congenital heart disease gene candidates: focus on RPL13. Schroeder AM, et al. Hum Mol Genet, 2019 Dec 1. PMID 31625562, Free PMC Article
  4. Identifying USPs regulating immune signals in Drosophila: USP2 deubiquitinates Imd and promotes its degradation by interacting with the proteasome. Engel E, et al. Cell Commun Signal, 2014 Jul 16. PMID 25027767, Free PMC Article
  5. High throughput in vivo functional validation of candidate congenital heart disease genes in Drosophila. Zhu JY, et al. Elife, 2017 Jan 20. PMID 28084990, Free PMC Article

See all (46) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Usp5, Usp34, and Otu1 deubiquitylases mediate DNA repair in Drosophila melanogaster.
    Title: Usp5, Usp34, and Otu1 deubiquitylases mediate DNA repair in Drosophila melanogaster.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by FlyBase

Function Evidence Code Pubs
enables cysteine-type deubiquitinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables cysteine-type deubiquitinase activity IEA
Inferred from Electronic Annotation
more info
  
enables cysteine-type deubiquitinase activity ISS
Inferred from Sequence or Structural Similarity
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in negative regulation of Toll signaling pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of antimicrobial peptide production IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of innate immune response IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of peptidoglycan recognition protein signaling pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of cell growth IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in positive regulation of cell growth IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of double-strand break repair via homologous recombination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in post-translational protein modification IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein deubiquitination IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of protein stability IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of protein stability IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in wing disc development IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
puffyeye
Names
CG5794-PD
CG5794-PE
CG5794-PF
CG5794-PG
CG5794-PH
CG5794-PI
puf-PD
puf-PE
puf-PF
puf-PG
puf-PH
puf-PI
NP_001262922.1
NP_001262923.1
NP_001287508.1
NP_651275.1
NP_733022.2
NP_996287.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NT_033777.3 Reference assembly

    Range
    24635270..24650043 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001275993.2 → NP_001262922.1  puffyeye, isoform G [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001262922.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A0B4KHF3
    Conserved Domains (4) summary
    cd02659
    Location:2013 → 2382
    peptidase_C19C; A subfamily of Peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
    pfam00443
    Location:2014 → 2377
    UCH; Ubiquitin carboxyl-terminal hydrolase
    pfam12030
    Location:2723 → 2877
    DUF3517; Domain of unknown function (DUF3517)
    cl19417
    Location:3671 → 3759
    FYDLN_acid; Protein of unknown function (FYDLN_acid)

  2. NM_170158.3 → NP_733022.2  puffyeye, isoform F [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_733022.2

    Status: REVIEWED

    UniProtKB/TrEMBL
    Q8IMW2
    Conserved Domains (3) summary
    cd02659
    Location:2013 → 2382
    peptidase_C19C; A subfamily of Peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
    pfam00443
    Location:2014 → 2377
    UCH; Ubiquitin carboxyl-terminal hydrolase
    pfam12030
    Location:2723 → 2877
    DUF3517; Domain of unknown function (DUF3517)

  3. NM_001300579.1 → NP_001287508.1  puffyeye, isoform I [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001287508.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A0B4LIK5
    Conserved Domains (4) summary
    cd02659
    Location:2013 → 2382
    peptidase_C19C; A subfamily of Peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
    pfam00443
    Location:2014 → 2377
    UCH; Ubiquitin carboxyl-terminal hydrolase
    pfam12030
    Location:2723 → 2877
    DUF3517; Domain of unknown function (DUF3517)
    cl19417
    Location:3671 → 3759
    FYDLN_acid; Protein of unknown function (FYDLN_acid)

  4. NM_143018.4 → NP_651275.1  puffyeye, isoform D [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_651275.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    Q7KS15, Q9VC56
    Conserved Domains (2) summary
    cd02659
    Location:2013 → 2382
    peptidase_C19C; A subfamily of Peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
    pfam10243
    Location:3651 → 3849
    MIP-T3; Microtubule-binding protein MIP-T3

  5. NM_001275994.2 → NP_001262923.1  puffyeye, isoform H [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001262923.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A0B4KI06
    Conserved Domains (3) summary
    cd02659
    Location:2013 → 2382
    peptidase_C19C; A subfamily of Peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
    pfam00443
    Location:2014 → 2377
    UCH; Ubiquitin carboxyl-terminal hydrolase
    pfam12030
    Location:2723 → 2877
    DUF3517; Domain of unknown function (DUF3517)

  6. NM_206564.2 → NP_996287.1  puffyeye, isoform E [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_996287.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    Q9VC56
    Conserved Domains (3) summary
    cd02659
    Location:2013 → 2382
    peptidase_C19C; A subfamily of Peptidase C19. Peptidase C19 contains ubiquitinyl hydrolases. They are intracellular peptidases that remove ubiquitin molecules from polyubiquinated peptides by cleavage of isopeptide bonds. They hydrolyze bonds involving the carboxyl ...
    pfam00443
    Location:2014 → 2377
    UCH; Ubiquitin carboxyl-terminal hydrolase
    pfam12030
    Location:2723 → 2817
    DUF3517; Domain of unknown function (DUF3517)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
A0A0B4KHF3 A0A0B4KHF3
A0A0B4KI06 A0A0B4KI06
A0A0B4LIK5 A0A0B4LIK5
Q8IH89 Q8IH89
Q8IMW2 Q8IMW2
Q8T8T3 Q8T8T3
Q9VC56.2 Q9VC56

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