Gen XPG-like endonuclease [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Genprovided by FlyBase
Official Full Name: XPG-like endonucleaseprovided by FlyBase
Primary source: FLYBASE:FBgn0263831
Locus tag: Dmel_CG10670
See related: AllianceGenome:FB:FBgn0263831
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: BcDNA:GM10765; CG10670; Dmel\CG10670; DmGen; DmGEN; GEN; mus324
Summary: Enables DNA nuclease activity and four-way junction DNA binding activity. Involved in double-strand break repair. Located in cytoplasm and nucleus. Is expressed in several structures, including antennal anlage; antennal primordium; central brain anlage; ganglia; and head epidermis primordium. Orthologous to human GEN1 (GEN1 Holliday junction 5' flap endonuclease). [provided by Alliance of Genome Resources, Jan 2025]
Orthologs: all
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Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3L	NT_037436.4 (5149849..5152376, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3L	NT_037436.3 (5142949..5145476, complement)

Chromosome 3L - NT_037436.4 Genomic Context describing neighboring genes

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

the in vitro activities of DmGen and HsGEN1 are strikingly similar.
Title: Substrate preference of Gen endonucleases highlights the importance of branched structures as DNA damage repair intermediates.
DmGEN belongs to a new class (class IV) of the RAD2/XPG nuclease family
Title: DmGEN shows a flap endonuclease activity, cleaving the blocked-flap structure and model replication fork.
Cloning and characterization of DmGEN, Drosophila Melanogaster XPG-like endonuclease, a RAD2 nuclease family member.
Title: DmGEN, a novel RAD2 family endo-exonuclease from Drosophila melanogaster.

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

Function	Evidence Code	Pubs
enables 5'-3' DNA exonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables 5'-flap endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables 5'-flap endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables crossover junction DNA endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables double-stranded DNA 3'-5' DNA exonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables four-way junction DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables four-way junction DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables nuclease activity	IEA Inferred from Electronic Annotation more info
enables single-stranded DNA 3'-5' DNA exonuclease activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA repair	IGI Inferred from Genetic Interaction more info	PubMed
involved_in DNA repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

NP_647943.2

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The following sections contain reference sequences that belong to a specific genome build. Explain