robo1 roundabout 1 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: robo1provided by FlyBase
Official Full Name: roundabout 1provided by FlyBase
Primary source: FLYBASE:FBgn0005631
Locus tag: Dmel_CG13521
See related: AllianceGenome:FB:FBgn0005631
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: BEST:GM07780; CG13521; CT32892; D-Robo1; Dmel\CG13521; dRobo; dRobo1; robo; Robo; ROBO; robo-1; Robo-1; Robo1; robo1S; roundabout
Summary: Enables heparin binding activity. Involved in several processes, including heart development; neuron projection guidance; and regulation of cell migration. Located in several cellular components, including axonal growth cone; dendrite; and neuronal cell body. Part of Slit-Robo signaling complex. Is expressed in several structures, including CNS neuron; epithelium; larval longitudinal connective; sensory neurons; and synaptic neuropil domain. Used to study myoclonic-atonic epilepsy. Human ortholog(s) of this gene implicated in congenital nystagmus (multiple); hematologic cancer (multiple); panhypopituitarism; and vesicoureteral reflux. Orthologous to several human genes including ROBO1 (roundabout guidance receptor 1). [provided by Alliance of Genome Resources, Jan 2025]
Orthologs: all
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Genomic context

Location:: 59A2-59A3; 2-101 cM

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (22692524..22700970, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (18580029..18588475, complement)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Robo recruitment of the Wave regulatory complex plays an essential and conserved role in midline repulsion.
Title: Robo recruitment of the Wave regulatory complex plays an essential and conserved role in midline repulsion.
Conserved and divergent aspects of Robo receptor signaling and regulation between Drosophila Robo1 and C. elegans SAX-3.
Title: Conserved and divergent aspects of Robo receptor signaling and regulation between Drosophila Robo1 and C. elegans SAX-3.
Results indicate that each of the Drosophila Roundabout (Robo1) Fibronectin (Fn) repeats are individually dispensable for the protein's role in midline repulsion.
Title: In Vivo Functional Analysis of Drosophila Robo1 Fibronectin Type-III Repeats.
Vascular cells produce Slit that activates Robo receptors in the posterior signalling centre of the larval haematopoietic organ, the lymph gland.
Title: Vascular control of the Drosophila haematopoietic microenvironment by Slit/Robo signalling.
Mmy is required for Slit secretion and for maintaining Robo abundance and distribution in the developing nervous system in Drosophila.
Title: The glycosylation pathway is required for the secretion of Slit and for the maintenance of the Slit receptor Robo on axons.
Dscam1 appears to modify the output of Robo and Slit complexes so that signaling is no longer repulsive
Title: Dscam1 Forms a Complex with Robo1 and the N-Terminal Fragment of Slit to Promote the Growth of Longitudinal Axons.
loss of Atg16 or its binding partner Rab19 impairs Slit production, which normally inhibits enteroendocrine cell generation by activating Robo signaling in stem cells.
Title: Drosophila Atg16 promotes enteroendocrine cell differentiation via regulation of intestinal Slit/Robo signaling.
Findings indicate that four of the five immunoglobulin-like (Ig) domains within Drosophila Robo1 are dispensable for its role in midline repulsion, despite their strong evolutionary conservation, and highlight a unique requirement for the Slit-binding Ig1 domain in the regulation of midline crossing.
Title: In vivo functional analysis of Drosophila Robo1 immunoglobulin-like domains.
FruBM employs the Robo1 pathway for formation of the male-specific ipsilateral neurite
Title: Fruitless Represses robo1 Transcription to Shape Male-Specific Neural Morphology and Behavior in Drosophila.
Deleting Ig1 from Robo1 disrupts Slit binding in cultured Drosophila cells. Robo1 variant lacking Ig1 (Robo1Ig1) is unable to promote ectopic midline repulsion.
Title: Slit Binding via the Ig1 Domain Is Essential for Midline Repulsion by Drosophila Robo1 but Dispensable for Receptor Expression, Localization, and Regulation in Vivo.

Submit: New GeneRIF Correction See all GeneRIFs (40)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables axon guidance receptor activity	IEA Inferred from Electronic Annotation more info
enables heparin binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Roundabout signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in Roundabout signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in axon guidance	IEA Inferred from Electronic Annotation more info
involved_in axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in axon guidance	IPI Inferred from Physical Interaction more info	PubMed
involved_in axon midline choice point recognition	IEA Inferred from Electronic Annotation more info
involved_in axon midline choice point recognition	IGI Inferred from Genetic Interaction more info	PubMed
involved_in axon midline choice point recognition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dendrite guidance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dendrite morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in embryonic heart tube development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in entrainment of circadian clock	IDA Inferred from Direct Assay more info	PubMed
involved_in gonad development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in motor neuron axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in muscle cell cellular homeostasis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in neuron recognition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in outflow tract morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in photoreceptor cell axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cell-cell adhesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of dendrite development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of epithelial cell migration, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in salivary gland boundary specification	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Slit-Robo signaling complex	IDA Inferred from Direct Assay more info	PubMed
located_in axon	IDA Inferred from Direct Assay more info	PubMed
located_in axonal growth cone	IDA Inferred from Direct Assay more info	PubMed
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info	PubMed
part_of protein-containing complex	IPI Inferred from Physical Interaction more info	PubMed
located_in vesicle	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: roundabout 1

Names: CG13521-PA; CG13521-PC; robo; robo1-PA; robo1-PC; roundabout

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

22692524..22700970 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_057551.4 → NP_476899.1 roundabout 1, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_476899.1

Status: REVIEWED

UniProtKB/TrEMBL

O44924, Q7KVK3, Q9W213

Related

FBpp0071833

Conserved Domains (8) summary

cd05724
Location:159 → 251

Ig2_Robo; Second immunoglobulin (Ig)-like domain in Robo (roundabout) receptors

cd05725
Location:272 → 341

Ig3_Robo; Third immunoglobulin (Ig)-like domain in Robo (roundabout) receptors

smart00408
Location:459 → 521

IGc2; Immunoglobulin C-2 Type

smart00410
Location:351 → 436

IG_like; Immunoglobulin like

cd00063
Location:549 → 637

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

pfam07679
Location:56 → 150

I-set; Immunoglobulin I-set domain

pfam00041
Location:770 → 855

fn3; Fibronectin type III domain

cl11960
Location:56 → 151

Ig; Immunoglobulin domain
NM_166543.2 → NP_726224.3 roundabout 1, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_726224.3

Status: REVIEWED

UniProtKB/TrEMBL

O44924, Q7KVK3, Q9W213

Conserved Domains (8) summary

cd05724
Location:159 → 251

Ig2_Robo; Second immunoglobulin (Ig)-like domain in Robo (roundabout) receptors

cd05725
Location:272 → 341

Ig3_Robo; Third immunoglobulin (Ig)-like domain in Robo (roundabout) receptors

smart00408
Location:459 → 521

IGc2; Immunoglobulin C-2 Type

smart00410
Location:351 → 436

IG_like; Immunoglobulin like

cd00063
Location:549 → 637

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

pfam07679
Location:56 → 150

I-set; Immunoglobulin I-set domain

pfam00041
Location:770 → 855

fn3; Fibronectin type III domain

cl11960
Location:56 → 151

Ig; Immunoglobulin domain