pAbp poly(A) binding protein [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: pAbpprovided by FlyBase
Official Full Name: poly(A) binding proteinprovided by FlyBase
Primary source: FLYBASE:FBgn0265297
Locus tag: Dmel_CG5119
See related: AllianceGenome:FB:FBgn0265297
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: anon-EST:fe2E5; anon-EST:Posey247; anon-EST:Posey249; BABP; BcDNA:LD24412; CG16803; CG5119; Dmel\CG5119; dPABP; duo; l(2)k10109; l(2)SH1908; l(2)SH2 1908; pAB; pabp; pABp; pABP; Pabp; PAbp; PABP; PABP55B; PABPC1
Summary: Enables mRNA 3'-UTR binding activity and poly(A) binding activity. Involved in several processes, including germ cell development; male meiosis cytokinesis; and regulation of compound eye photoreceptor development. Located in cytoplasm. Part of ribonucleoprotein complex. Is expressed in several structures, including adult head; adult heart; dorsal-ventral compartment boundary of the wing disc; germline cell; and ovary. Orthologous to several human genes including PABPC1 (poly(A) binding protein cytoplasmic 1) and PABPC3 (poly(A) binding protein cytoplasmic 3). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 55B8-55B9; 2-84 cM

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (18140078..18147191)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (14027583..14034696)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PABP association with mRNA rapidly diminished with miRNA-induced silencing complex recruitment and before detectable deadenylation.
Title: PABP and the poly(A) tail augment microRNA repression by facilitated miRISC binding.
Single-molecule nanopore force spectroscopy was used to directly measure interactions between poly(A) and poly(A) binding protein.
Title: Nanopore detachment kinetics of poly(A) binding proteins from RNA molecules reveals the critical role of C-terminus interactions.
PABP generally modulates poly(A) length and translation efficiency, neither PABP function nor GW182-PABP interaction is a prerequisite for these two silencing pathways.
Title: PABP is not essential for microRNA-mediated translational repression and deadenylation in vitro.
Pabp binds to A-rich sequences (ARS) in the osk 3'UTR and these turned out to be required in vivo for osk function during early oogenesis.
Title: Pabp binds to the osk 3'UTR and specifically contributes to osk mRNA stability and oocyte accumulation.
These findings reveal that despite species-specific differences in the relative strength of the PABPC1-binding sites, the interaction between GW182 proteins and PABPC1 is critical for miRNA-mediated silencing in animal cells.
Title: Two PABPC1-binding sites in GW182 proteins promote miRNA-mediated gene silencing.
Drosophila Larp associates with poly(A)-binding protein and is required for male fertility and syncytial embryo development
Title: Drosophila Larp associates with poly(A)-binding protein and is required for male fertility and syncytial embryo development.
Efficient msl-2 mRNA silencing via the 3' UTR requires both a poly(A) tail and PABP function, indicating that UNR directly interacts with PABP.
Title: The SXL-UNR corepressor complex uses a PABP-mediated mechanism to inhibit ribosome recruitment to msl-2 mRNA.
model proposed in which the GW182 silencing domain promotes translational repression, at least in part, by interfering with mRNA circularization and also recruits the deadenylase complex through the interaction with PABPC1
Title: The silencing domain of GW182 interacts with PABPC1 to promote translational repression and degradation of microRNA targets and is required for target release.
Three proteins (DDP1, Hrp48, and poly(A)-binding protein) are components of the HDE-binding complex and function in translational enhancement
Title: A multiprotein complex that mediates translational enhancement in Drosophila.
Cytoplasmic poly(A)-binding protein 1 (PABPC1) has a conserved role in nonsense-mediated mRNA decay.
Title: A conserved role for cytoplasmic poly(A)-binding protein 1 (PABPC1) in nonsense-mediated mRNA decay.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables mRNA 3'-UTR binding	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA 3'-UTR binding	IDA Inferred from Direct Assay more info	PubMed
enables mRNA binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables poly(A) binding	IBA Inferred from Biological aspect of Ancestor more info
enables poly(A) binding	IDA Inferred from Direct Assay more info	PubMed
enables poly(A) binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables poly(A) binding	TAS Traceable Author Statement more info	PubMed
enables poly(U) RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in chemical synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dorsal/ventral pattern formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA splicing, via spliceosome	IC Inferred by Curator more info	PubMed
involved_in male meiosis cytokinesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in male meiotic nuclear division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oocyte development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of translation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of translation	TAS Traceable Author Statement more info	PubMed
involved_in regulation of compound eye photoreceptor development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in spermatid nucleus differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spermatogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of catalytic step 2 spliceosome	HDA more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasmic stress granule	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	HDA more info	PubMed
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
part_of precatalytic spliceosome	HDA more info	PubMed
part_of ribonucleoprotein complex	IBA Inferred from Biological aspect of Ancestor more info
part_of ribonucleoprotein complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: poly(A) binding protein

Names: CG5119-PA; CG5119-PB; CG5119-PC; Poly(A) binding protein; Poly(A)-binding protein; Poly(A)binding protein; PolyA-binding protein; Polyadenylate-binding protein; anon-fast-evolving-2E5; cytoplasmic poly-A binding protein; doppio fuso; lethal (2) SH1908; pAbp-PA; pAbp-PB; pAbp-PC; poly (A)-binding protein; poly-A binding protein

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

18140078..18147191

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_166265.2 → NP_725750.1 poly(A) binding protein, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_725750.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A4UZM5, P21187, Q0E932, Q9V8C3

Related

FBpp0085915

Conserved Domains (1) summary

TIGR01628
Location:2 → 625

PABP-1234; polyadenylate binding protein, human types 1, 2, 3, 4 family
NM_057319.5 → NP_476667.1 poly(A) binding protein, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_476667.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A4UZM5, P21187, Q0E932, Q9V8C3

Related

FBpp0085917

Conserved Domains (1) summary

TIGR01628
Location:2 → 625

PABP-1234; polyadenylate binding protein, human types 1, 2, 3, 4 family
NM_166264.4 → NP_725749.1 poly(A) binding protein, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_725749.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A4UZM5, P21187, Q0E932, Q9V8C3

Related

FBpp0085919

Conserved Domains (1) summary

TIGR01628
Location:2 → 625

PABP-1234; polyadenylate binding protein, human types 1, 2, 3, 4 family