RhoGEF2 Rho guanine nucleotide exchange factor 2 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: RhoGEF2provided by FlyBase
Official Full Name: Rho guanine nucleotide exchange factor 2provided by FlyBase
Primary source: FLYBASE:FBgn0023172
Locus tag: Dmel_CG9635
See related: AllianceGenome:FB:FBgn0023172
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG9635; Dmel\CG9635; dRhoGEF; DRhoGEF; dRhoGEF2; DrhoGEF2; DRhoGEF2; Gef2; l(2)04291; RG2; Rho-GEF2; RhoGEF; rhogef2; rhoGEF2; RhoGef2; RhoGF2; shar; Su(Rho1)2B; T2
Summary: Enables guanyl-nucleotide exchange factor activity. Involved in several processes, including cell elongation involved in imaginal disc-derived wing morphogenesis; gastrulation involving germ band extension; and positive regulation of Rho protein signal transduction. Located in several cellular components, including apical cortex; cortical actin cytoskeleton; and lateral plasma membrane. Is expressed in several structures, including epithelium; gut section; lamina; larval brain; and organism subdivision. Human ortholog(s) of this gene implicated in immunodeficiency 62. Orthologous to several human genes including ARHGEF11 (Rho guanine nucleotide exchange factor 11). [provided by Alliance of Genome Resources, Jan 2025]
Orthologs: all
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Genomic context

Location:: 53E4-53F1; 2-81 cM

Exon count:: 24

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (17027190..17044653)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (12914695..12932158)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Centralspindlin-mediated transport of RhoGEF positions the cleavage plane for cytokinesis.
Title: Centralspindlin-mediated transport of RhoGEF positions the cleavage plane for cytokinesis.
Increased lateral tension is sufficient for epithelial folding in Drosophila.
Title: Increased lateral tension is sufficient for epithelial folding in Drosophila.
Distinct RhoGEFs Activate Apical and Junctional Contractility under Control of G Proteins during Epithelial Morphogenesis.
Title: Distinct RhoGEFs Activate Apical and Junctional Contractility under Control of G Proteins during Epithelial Morphogenesis.
Recruitment of RhoGEF2 to the plasma membrane is sufficient to cause apical constriction and tissue-level responses that are compatible with endogenous invagination processes.
Title: Guided morphogenesis through optogenetic activation of Rho signalling during early Drosophila embryogenesis.
RhoGEF2, Pbl, RhoGEF3, and Tum exhibit discrete localization patterns and are required for cell wound repair.
Title: Prepatterning by RhoGEFs governs Rho GTPase spatiotemporal dynamics during wound repair.
Overexpression of DRhoGEF2, and it's functional mammalian ortholog PDZ-RhoGEF (ArhGEF11), at various stages of eye development, resulted in both dPKB/Akt-dependent and -independent phenotypes in Drosophila
Title: The Rho Guanine Nucleotide Exchange Factor DRhoGEF2 Is a Genetic Modifier of the PI3K Pathway in Drosophila.
RhoGEF2 also cooperates with an activated allele of a downstream effector of Ras, Raf (Raf(GOF)).
Title: In Drosophila, RhoGEF2 cooperates with activated Ras in tumorigenesis through a pathway involving Rho1-Rok-Myosin-II and JNK signalling.
early Drosophila embryogenesis is primed to form furrows at either overlapping astral microtubules or the central spindle; the shift to the latter is driven by a corresponding shift from RhoGEF2 to Pebble in controlling furrow formation.
Title: RhoGEF and positioning of rappaport-like furrows in the early Drosophila embryo.
Frizzled-dependent planar polarity pathway locally promotes E-cadherin turnover via recruitment of RhoGEF2.
Title: The Frizzled-dependent planar polarity pathway locally promotes E-cadherin turnover via recruitment of RhoGEF2.
Results identify DRhoGEF2 as an upstream regulator of the actomyosin contractile machinery that drives amnioserosa cells pulsations and apical constriction.
Title: DRhoGEF2 regulates cellular tension and cell pulsations in the Amnioserosa during Drosophila dorsal closure.

Submit: New GeneRIF Correction See all GeneRIFs (20)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables G protein-coupled receptor binding	IBA Inferred from Biological aspect of Ancestor more info
enables guanyl-nucleotide exchange factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables guanyl-nucleotide exchange factor activity	IEA Inferred from Electronic Annotation more info
enables guanyl-nucleotide exchange factor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables guanyl-nucleotide exchange factor activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed

Process	Evidence Code	Pubs
involved_in G protein-coupled receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin filament organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin-mediated cell contraction	IGI Inferred from Genetic Interaction more info	PubMed
involved_in anterior midgut invagination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in apical constriction involved in ventral furrow formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell elongation involved in imaginal disc-derived wing morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellularization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in germ-band extension	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in morphogenesis of embryonic epithelium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pole cell development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of Rho protein signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in posterior midgut invagination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pseudocleavage involved in syncytial blastoderm formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of actomyosin contractile ring contraction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of axonogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of cell shape	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of embryonic cell shape	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of embryonic cell shape	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of myosin II filament organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to wounding	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spiracle morphogenesis, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ventral furrow formation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in actin cap	IDA Inferred from Direct Assay more info	PubMed
located_in actomyosin contractile ring	IDA Inferred from Direct Assay more info	PubMed
located_in apical cortex	IDA Inferred from Direct Assay more info	PubMed
located_in apical part of cell	IDA Inferred from Direct Assay more info	PubMed
located_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in basal part of cell	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in lateral plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Rho guanine nucleotide exchange factor 2

Names: CG9635-PD; CG9635-PE; CG9635-PF; CG9635-PG; CG9635-PH; RhoGEF2-PD; RhoGEF2-PE; RhoGEF2-PF; RhoGEF2-PG; RhoGEF2-PH; akkordion; guanine nucleotide exchange factor 2; shar pei; transcription unit 2

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

17027190..17044653

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_206146.4 → NP_995868.1 Rho guanine nucleotide exchange factor 2, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_995868.1

Status: REVIEWED

UniProtKB/TrEMBL

A1ZAN6, O44113

Conserved Domains (6) summary

cd08756
Location:934 → 1055

RGS_GEF_like; Regulator of G protein signaling (RGS) domain found in the Rho guanine nucleotide exchange factor (RhoGEF) protein

smart00228
Location:260 → 334

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

cd00160
Location:1539 → 1731

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd13329
Location:1770 → 1875

PH_RhoGEF; Rho guanine nucleotide exchange factor Pleckstrin homology domain

pfam00130
Location:1151 → 1202

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam10310
Location:2242 → 2378

DUF2413; Protein of unknown function (DUF2413)
NM_001259452.2 → NP_001246381.1 Rho guanine nucleotide exchange factor 2, isoform G [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246381.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4K7T7, O44113

Conserved Domains (6) summary

cd08756
Location:934 → 1055

RGS_GEF_like; Regulator of G protein signaling (RGS) domain found in the Rho guanine nucleotide exchange factor (RhoGEF) protein

smart00228
Location:260 → 334

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

cd00160
Location:1537 → 1729

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd13329
Location:1768 → 1873

PH_RhoGEF; Rho guanine nucleotide exchange factor Pleckstrin homology domain

pfam00130
Location:1151 → 1202

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam10310
Location:2240 → 2376

DUF2413; Protein of unknown function (DUF2413)
NM_206147.4 → NP_995869.1 Rho guanine nucleotide exchange factor 2, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_995869.1

Status: REVIEWED

UniProtKB/TrEMBL

A1ZAN7, O44113

Conserved Domains (6) summary

cd08756
Location:580 → 701

RGS_GEF_like; Regulator of G protein signaling (RGS) domain found in the Rho guanine nucleotide exchange factor (RhoGEF) protein

smart00228
Location:260 → 334

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

cd00160
Location:1185 → 1377

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd13329
Location:1416 → 1521

PH_RhoGEF; Rho guanine nucleotide exchange factor Pleckstrin homology domain

pfam00130
Location:797 → 848

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam10310
Location:1888 → 2024

DUF2413; Protein of unknown function (DUF2413)
NM_057969.6 → NP_477317.1 Rho guanine nucleotide exchange factor 2, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_477317.1

Status: REVIEWED

UniProtKB/TrEMBL

A1ZAN6, O44113

Related

FBpp0086125

Conserved Domains (6) summary

cd08756
Location:934 → 1055

RGS_GEF_like; Regulator of G protein signaling (RGS) domain found in the Rho guanine nucleotide exchange factor (RhoGEF) protein

smart00228
Location:260 → 334

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

cd00160
Location:1539 → 1731

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd13329
Location:1770 → 1875

PH_RhoGEF; Rho guanine nucleotide exchange factor Pleckstrin homology domain

pfam00130
Location:1151 → 1202

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam10310
Location:2242 → 2378

DUF2413; Protein of unknown function (DUF2413)
NM_001299586.1 → NP_001286515.1 Rho guanine nucleotide exchange factor 2, isoform H [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001286515.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4LFF8, O44113

Conserved Domains (6) summary

cd08756
Location:831 → 952

RGS_GEF_like; Regulator of G protein signaling (RGS) domain found in the Rho guanine nucleotide exchange factor (RhoGEF) protein

smart00228
Location:260 → 334

PDZ; Domain present in PSD-95, Dlg, and ZO-1/2

cd00160
Location:1436 → 1628

RhoGEF; Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases; Also called Dbl-homologous (DH) domain. It appears that PH domains invariably occur C-terminal to RhoGEF/DH domains.

cd13329
Location:1667 → 1772

PH_RhoGEF; Rho guanine nucleotide exchange factor Pleckstrin homology domain

pfam00130
Location:1048 → 1099

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)

pfam10310
Location:2139 → 2275

DUF2413; Protein of unknown function (DUF2413)