clu clueless [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: cluprovided by FlyBase
Official Full Name: cluelessprovided by FlyBase
Primary source: FLYBASE:FBgn0034087
Locus tag: Dmel_CG8443
See related: AllianceGenome:FB:FBgn0034087
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: anon-WO0118547.143; CG8443; Clu; Dmel\CG8443
Summary: Enables mRNA binding activity and ribosome binding activity. Involved in several processes, including asymmetric neuroblast division; mitochondrion localization; and ribosome localization. Is active in cytosol. Is expressed in several structures, including embryonic/larval midgut primordium; female germline cell; ganglia; larval muscle cell; and trunk visceral muscle primordium. Orthologous to human CLUH (clustered mitochondria homolog). [provided by Alliance of Genome Resources, Jan 2025]
Orthologs: all
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Genomic context

Location:: 52F5-52F7; 2-78 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (16186584..16194277)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (12074089..12081782)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Clueless forms dynamic, insulin-responsive bliss particles sensitive to stress.
Title: Clueless forms dynamic, insulin-responsive bliss particles sensitive to stress.
Clu is upstream of and binds to VCP in vivo and promotes VCP-dependent Marf degradation in vitro Marf accumulates in whole muscle lysates of clu-deficient flies and is destabilized upon Clu overexpression. Thus, Clu is essential for mitochondrial homeostasis and functions in concert with Parkin and VCP for Marf degradation to promote damaged mitochondrial clearance.
Title: Drosophila clueless is involved in Parkin-dependent mitophagy by promoting VCP-mediated Marf degradation.
Clu directly modulates mitochondrial function, and that Clu's function contributes to the PINK1-Park pathway of mitochondrial quality control.
Title: Clueless, a protein required for mitochondrial function, interacts with the PINK1-Parkin complex in Drosophila.
Clueless (clu) is a novel regulator of aPKC activity.
Title: Clueless regulates aPKC activity and promotes self-renewal cell fate in Drosophila lgl mutant larval brains.
The results suggest Clu functions upstream of electron transport and oxidative phosphorylation, has a role in suppressing oxidative damage in the cell, and that lack of Clu's specific function causes mitochondria to mislocalize.
Title: Drosophila clueless is highly expressed in larval neuroblasts, affects mitochondrial localization and suppresses mitochondrial oxidative damage.
strong clu mutations resemble park mutations in their effects on mitochondrial function and that the two genes interact genetically
Title: Clueless, a conserved Drosophila gene required for mitochondrial subcellular localization, interacts genetically with parkin.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

mobile_element

Loc: 16193899-16193962 mobile_element_type = transposon:INE-1{}6176

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables mRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables ribosome binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in asymmetric neuroblast division	IGI Inferred from Genetic Interaction more info	PubMed
involved_in asymmetric neuroblast division	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in asymmetric neuroblast division	IPI Inferred from Physical Interaction more info	PubMed
involved_in intracellular distribution of mitochondria	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitochondrion localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrion organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ribosome localization	IPI Inferred from Physical Interaction more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: clueless

Names: CG8443-PA; CG8443-PB; clu-PA; clu-PB

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

16186584..16194277

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_137251.3 → NP_611095.1 clueless, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_611095.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A1ZAB5, Q6NNE5

Related

FBpp0086380

Conserved Domains (7) summary

sd00006
Location:1190 → 1215

TPR; TPR repeat [structural motif]

pfam05303
Location:324 → 387

DUF727; Protein of unknown function (DUF727)

pfam12807
Location:872 → 1073

eIF3_p135; Translation initiation factor eIF3 subunit 135

pfam13236
Location:445 → 665

CLU; Clustered mitochondria

pfam13374
Location:1146 → 1184

TPR_10; Tetratricopeptide repeat

pfam13424
Location:1225 → 1303

TPR_12; Tetratricopeptide repeat

pfam15044
Location:159 → 232

CLU_N; Mitochondrial function, CLU-N-term
NM_001274092.1 → NP_001261021.1 clueless, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001261021.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KEX0

Conserved Domains (7) summary

sd00006
Location:1031 → 1056

TPR; TPR repeat [structural motif]

pfam05303
Location:165 → 228

DUF727; Protein of unknown function (DUF727)

pfam12807
Location:713 → 914

eIF3_p135; Translation initiation factor eIF3 subunit 135

pfam13236
Location:286 → 506

CLU; Clustered mitochondria

pfam13374
Location:987 → 1025

TPR_10; Tetratricopeptide repeat

pfam13424
Location:1066 → 1144

TPR_12; Tetratricopeptide repeat

pfam15044
Location:2 → 73

CLU_N; Mitochondrial function, CLU-N-term