Mlf Myelodysplasia/myeloid leukemia factor [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Mlfprovided by FlyBase
Official Full Name: Myelodysplasia/myeloid leukemia factorprovided by FlyBase
Primary source: FLYBASE:FBgn0034051
Locus tag: Dmel_CG8295
See related: AllianceGenome:FB:FBgn0034051
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: BcDNA:SD18148; CG8295; Dmel\CG8295; dmlf; dMLF; mlf; MLF
Summary: Enables DNA-binding transcription factor binding activity and protein-folding chaperone binding activity. Involved in several processes, including positive regulation of crystal cell differentiation; protein stabilization; and regulation of cell proliferation involved in compound eye morphogenesis. Located in nucleus; perinuclear region of cytoplasm; and polytene chromosome interband. Is expressed in several structures, including central brain primordium; embryonic/larval garland cell; embryonic/larval hemocyte; embryonic/larval midgut primordium; and ganglia. Orthologous to several human genes including MLF2 (myeloid leukemia factor 2). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 52D2-52D3; 2-76 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (15935893..15940073, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (11823398..11827578, complement)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

our data indicate that the MLF/DnaJ-1-dependent increase in Lz level allows the repression of Notch expression and signaling to prevent aberrant blood cell development. Thus our findings establish a functional link between MLF and the co-chaperone DnaJ-1 to control RUNX transcription factor activity and Notch signaling during blood cell development in vivo.
Title: Control of RUNX-induced repression of Notch signaling by MLF and its partner DnaJ-1 during Drosophila hematopoiesis.
mlf participates in a positive feedback loop to fine tune the activity of the RUNX transcription factor Lozenge (LZ) during development of the crystal cells.
Title: Myeloid leukemia factor is a conserved regulator of RUNX transcription factor activity involved in hematopoiesis.
Data revealed that MLF interacts with CSN3 in vivo and that the PCI domain of the dCSN3 protein is sufficient for this to occur, possibly functioning as a structural scaffold for assembly of the COP9 signalosome complex.
Title: The myeloid leukemia factor interacts with COP9 signalosome subunit 3 in Drosophila melanogaster.
MLF1 has been described as mainly cytoplasmic, but in Drosophila, one of the dMLF isoforms (dMLFA) localized mainly in the nucleus.
Title: Identification of novel nuclear localization signals of Drosophila myeloid leukemia factor.
Mlf and its mutant were characterized. Mlf can act developmentaly and post-developmentally to suppress neurodegeneration and premature aging in a cerebellar ataxia model.
Title: Characterization of the Drosophila myeloid leukemia factor.
role in the control of cell growth in eye development
Title: A genomic switch at the transition from cell proliferation to terminal differentiation in the Drosophila eye.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-folding chaperone binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in positive regulation of crystal cell differentiation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of crystal cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein stabilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell proliferation involved in compound eye morphogenesis	IEP Inferred from Expression Pattern more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in polytene chromosome interband	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Myelodysplasia/myeloid leukemia factor

Names: CG8295-PA; CG8295-PB; CG8295-PC; CG8295-PD; CG8295-PE; CG8295-PF; CG8295-PG; CG8295-PH; Mlf-PA; Mlf-PB; Mlf-PC; Mlf-PD; Mlf-PE; Mlf-PF; Mlf-PG; Mlf-PH; drosophila myeloid leukemia factor; myeloid leukemia factor

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

15935893..15940073 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_206125.2 → NP_995847.1 Myelodysplasia/myeloid leukemia factor, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_995847.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A1ZA61, A1ZA62, Q540Y3, Q8IG90, Q95P43, Q9NKV0, Q9V7G3

UniProtKB/TrEMBL

C0PUX0

Conserved Domains (1) summary

pfam10248
Location:37 → 199

Mlf1IP; Myelodysplasia-myeloid leukemia factor 1-interacting protein
NM_001169693.1 → NP_001163164.1 Myelodysplasia/myeloid leukemia factor, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001163164.1

Status: REVIEWED

UniProtKB/TrEMBL

C0PUX0, E1JH79

Conserved Domains (1) summary

pfam10248
Location:37 → 199

Mlf1IP; Myelodysplasia-myeloid leukemia factor 1-interacting protein
NM_166123.4 → NP_725502.1 Myelodysplasia/myeloid leukemia factor, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_725502.1

Status: REVIEWED

UniProtKB/TrEMBL

D3DMK9

Related

FBpp0086429

Conserved Domains (1) summary

pfam10248
Location:37 → 199

Mlf1IP; Myelodysplasia-myeloid leukemia factor 1-interacting protein
NM_079029.5 → NP_523753.1 Myelodysplasia/myeloid leukemia factor, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_523753.1

Status: REVIEWED

UniProtKB/TrEMBL

D3DMK9

Related

FBpp0086428

Conserved Domains (1) summary

pfam10248
Location:37 → 199

Mlf1IP; Myelodysplasia-myeloid leukemia factor 1-interacting protein
NM_001259425.1 → NP_001246354.1 Myelodysplasia/myeloid leukemia factor, isoform G [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246354.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4K807, D3DN03

Conserved Domains (1) summary

pfam10248
Location:16 → 178

Mlf1IP; Myelodysplasia-myeloid leukemia factor 1-interacting protein
NM_166122.4 → NP_725501.2 Myelodysplasia/myeloid leukemia factor, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_725501.2

Status: REVIEWED

UniProtKB/TrEMBL

D3DN03

Related

FBpp0086430

Conserved Domains (1) summary

pfam10248
Location:35 → 197

Mlf1IP; Myelodysplasia-myeloid leukemia factor 1-interacting protein
NM_001259424.2 → NP_001246353.1 Myelodysplasia/myeloid leukemia factor, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246353.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4K7R5, D3DN03

Conserved Domains (1) summary

pfam10248
Location:35 → 197

Mlf1IP; Myelodysplasia-myeloid leukemia factor 1-interacting protein
NM_001259426.2 → NP_001246355.1 Myelodysplasia/myeloid leukemia factor, isoform H [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246355.1

Status: REVIEWED

UniProtKB/TrEMBL

C0PUX0, E1JH79

Conserved Domains (1) summary

pfam10248
Location:37 → 199

Mlf1IP; Myelodysplasia-myeloid leukemia factor 1-interacting protein