sli slit [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: sliprovided by FlyBase
Official Full Name: slitprovided by FlyBase
Primary source: FLYBASE:FBgn0264089
Locus tag: Dmel_CG43758
See related: AllianceGenome:FB:FBgn0264089
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG33464; CG43758; CG8355; CT21700; CT37068; Dmel\CG43758; dSlit; fus3; l(2)05248; Sli; Slit; wdh
Old locus tag: Dmel_CG33464; Dmel_CG8355
Summary: Enables Roundabout binding activity and heparin binding activity. Involved in several processes, including heart development; neurogenesis; and regulation of cell migration. Located in axon; cell surface; and extracellular region. Part of Slit-Robo signaling complex. Is expressed in several structures, including central nervous system; ganglia; glial cell; larval multidendritic neurons; and tract. Orthologous to human SLIT1 (slit guidance ligand 1); SLIT2 (slit guidance ligand 2); and SLIT3 (slit guidance ligand 3). [provided by Alliance of Genome Resources, Jan 2025]
Orthologs: all
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Genomic context

Location:: 52C9-52D2; 2-76 cM

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2R	NT_033778.4 (15869495..15922118, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2R	NT_033778.3 (11757000..11809623, complement)

Chromosome 2R - NT_033778.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Proteolytic cleavage of Slit by the Tolkin protease converts an axon repulsion cue to an axon growth cue in vivo.
Title: Proteolytic cleavage of Slit by the Tolkin protease converts an axon repulsion cue to an axon growth cue in vivo.
Slit neuronal secretion coordinates optic lobe morphogenesis in Drosophila.
Title: Slit neuronal secretion coordinates optic lobe morphogenesis in Drosophila.
Vascular cells produce Slit that activates Robo receptors in the posterior signalling centre of the larval haematopoietic organ, the lymph gland.
Title: Vascular control of the Drosophila haematopoietic microenvironment by Slit/Robo signalling.
Mmy is required for Slit secretion and for maintaining Robo abundance and distribution in the developing nervous system in Drosophila.
Title: The glycosylation pathway is required for the secretion of Slit and for the maintenance of the Slit receptor Robo on axons.
Dscam1 appears to modify the output of Robo and Slit complexes so that signaling is no longer repulsive
Title: Dscam1 Forms a Complex with Robo1 and the N-Terminal Fragment of Slit to Promote the Growth of Longitudinal Axons.
loss of Atg16 or its binding partner Rab19 impairs Slit production, which normally inhibits enteroendocrine cell generation by activating Robo signaling in stem cells.
Title: Drosophila Atg16 promotes enteroendocrine cell differentiation via regulation of intestinal Slit/Robo signaling.
repulsive Slit-Robo2-Ena signaling exerts an extrusive force downstream of JNK to eliminate scrib cells from epithelia by disrupting E-cadherin.
Title: Slit-Robo Repulsive Signaling Extrudes Tumorigenic Cells from Epithelia.
the role of Slit and Robo receptors in wiring Drosophila higher-order brain circuits and identified differences in the cellular and molecular mechanisms of Robo/Slit function, was investigated.
Title: Regulation of Drosophila Brain Wiring by Neuropil Interactions via a Slit-Robo-RPTP Signaling Complex.
These results suggest that Slit cleaveage, and the formation of Slit-N oligomers on the tendon cell surfaces direct muscle elongation, provide a stop signal for the approaching muscle.
Title: Cleaved Slit directs embryonic muscles.
Slit-binding is necessary for its repulsive signaling output
Title: Slit-Dependent Endocytic Trafficking of the Robo Receptor Is Required for Son of Sevenless Recruitment and Midline Axon Repulsion.

Submit: New GeneRIF Correction See all GeneRIFs (35)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables Roundabout binding	IBA Inferred from Biological aspect of Ancestor more info
enables Roundabout binding	IPI Inferred from Physical Interaction more info	PubMed
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables heparin binding	IBA Inferred from Biological aspect of Ancestor more info
enables heparin binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in Roundabout signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in axon guidance	IBA Inferred from Biological aspect of Ancestor more info
involved_in axon guidance	IGI Inferred from Genetic Interaction more info	PubMed
involved_in axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in axon guidance	IPI Inferred from Physical Interaction more info	PubMed
involved_in axon midline choice point recognition	IGI Inferred from Genetic Interaction more info	PubMed
involved_in axon midline choice point recognition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dendrite morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in digestive tract mesoderm development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in embryonic heart tube development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in epithelial cell migration, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glial cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in gonad development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in induction of negative chemotaxis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mesoderm migration involved in gastrulation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mesodermal cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuron differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuron migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in outflow tract morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cell-cell adhesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of epithelial cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of epithelial cell migration, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in salivary gland boundary specification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in synaptic target inhibition	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Slit-Robo signaling complex	IDA Inferred from Direct Assay more info	PubMed
located_in axon	IDA Inferred from Direct Assay more info	PubMed
located_in cell surface	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular region	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: slit

Names: CG43758-PA; CG43758-PB; CG43758-PC; CG43758-PD; CG43758-PE; CG43758-PF; SGP cluster fusion defects 3; lethal (2) 05248; sli-PA; sli-PB; sli-PC; sli-PD; sli-PE; sli-PF

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.4 Reference assembly

Range

15869495..15922118 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001103863.3 → NP_001097333.1 slit, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001097333.1

Status: REVIEWED

UniProtKB/TrEMBL

E1NZD5

Conserved Domains (12) summary

smart00370
Location:813 → 836

LRR; Leucine-rich repeats, outliers

smart00013
Location:518 → 550

LRRNT; Leucine rich repeat N-terminal domain

smart00041
Location:1411 → 1480

CT; C-terminal cystine knot-like domain (CTCK)

smart00082
Location:653 → 702

LRRCT; Leucine rich repeat C-terminal domain

cd00054
Location:1064 → 1100

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...

cd00116
Location:550 → 654

LRR_RI; Leucine-rich repeats (LRRs), ribonuclease inhibitor (RI)-like subfamily. LRRs are 20-29 residue sequence motifs present in many proteins that participate in protein-protein interactions and have different functions and cellular locations. LRRs correspond ...

sd00031
Location:444 → 456

LRR_1; leucine-rich repeat [structural motif]

sd00033
Location:83 → 104

LRR_RI; leucine-rich repeat [structural motif]

pfam00008
Location:911 → 942

EGF; EGF-like domain

pfam00054
Location:1180 → 1311

Laminin_G_1; Laminin G domain

pfam12799
Location:347 → 387

LRR_4; Leucine Rich repeats (2 copies)

pfam13855
Location:766 → 826

LRR_8; Leucine rich repeat
NM_057380.4 → NP_476728.1 slit, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_476728.1

Status: REVIEWED

UniProtKB/TrEMBL

E1NZD5

Related

FBpp0303573

Conserved Domains (12) summary

smart00370
Location:813 → 836

LRR; Leucine-rich repeats, outliers

smart00013
Location:518 → 550

LRRNT; Leucine rich repeat N-terminal domain

smart00041
Location:1411 → 1480

CT; C-terminal cystine knot-like domain (CTCK)

smart00082
Location:653 → 702

LRRCT; Leucine rich repeat C-terminal domain

cd00054
Location:1064 → 1100

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...

cd00116
Location:550 → 654

LRR_RI; Leucine-rich repeats (LRRs), ribonuclease inhibitor (RI)-like subfamily. LRRs are 20-29 residue sequence motifs present in many proteins that participate in protein-protein interactions and have different functions and cellular locations. LRRs correspond ...

sd00031
Location:444 → 456

LRR_1; leucine-rich repeat [structural motif]

sd00033
Location:83 → 104

LRR_RI; leucine-rich repeat [structural motif]

pfam00008
Location:911 → 942

EGF; EGF-like domain

pfam00054
Location:1180 → 1311

Laminin_G_1; Laminin G domain

pfam12799
Location:347 → 387

LRR_4; Leucine Rich repeats (2 copies)

pfam13855
Location:766 → 826

LRR_8; Leucine rich repeat
NM_057379.4 → NP_476727.1 slit, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_476727.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A8DYF5, A8DYF6, P24014, Q24526, Q8MLB9, Q9V7F8, Q9V7F9, Q9XYV4

UniProtKB/TrEMBL

F0JAL2

Related

FBpp0303575

Conserved Domains (11) summary

smart00369
Location:837 → 860

LRR_TYP; Leucine-rich repeats, typical (most populated) subfamily

smart00013
Location:542 → 574

LRRNT; Leucine rich repeat N-terminal domain

smart00041
Location:1435 → 1504

CT; C-terminal cystine knot-like domain (CTCK)

smart00082
Location:677 → 726

LRRCT; Leucine rich repeat C-terminal domain

cd00054
Location:1088 → 1124

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...

cd00116
Location:119 → 259

LRR_RI; Leucine-rich repeats (LRRs), ribonuclease inhibitor (RI)-like subfamily. LRRs are 20-29 residue sequence motifs present in many proteins that participate in protein-protein interactions and have different functions and cellular locations. LRRs correspond ...

sd00033
Location:83 → 104

LRR_RI; leucine-rich repeat [structural motif]

pfam00008
Location:935 → 966

EGF; EGF-like domain

pfam00054
Location:1204 → 1335

Laminin_G_1; Laminin G domain

pfam12799
Location:371 → 411

LRR_4; Leucine Rich repeats (2 copies)

pfam13855
Location:790 → 850

LRR_8; Leucine rich repeat
NM_001274088.1 → NP_001261017.1 slit, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001261017.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KES0

Conserved Domains (11) summary

smart00013
Location:542 → 574

LRRNT; Leucine rich repeat N-terminal domain

smart00041
Location:1435 → 1504

CT; C-terminal cystine knot-like domain (CTCK)

smart00082
Location:677 → 726

LRRCT; Leucine rich repeat C-terminal domain

cd00054
Location:1088 → 1124

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...

cd00116
Location:119 → 259

LRR_RI; Leucine-rich repeats (LRRs), ribonuclease inhibitor (RI)-like subfamily. LRRs are 20-29 residue sequence motifs present in many proteins that participate in protein-protein interactions and have different functions and cellular locations. LRRs correspond ...

sd00031
Location:468 → 480

LRR_1; leucine-rich repeat [structural motif]

sd00033
Location:83 → 104

LRR_RI; leucine-rich repeat [structural motif]

pfam00008
Location:935 → 966

EGF; EGF-like domain

pfam00054
Location:1204 → 1335

Laminin_G_1; Laminin G domain

pfam12799
Location:371 → 411

LRR_4; Leucine Rich repeats (2 copies)

pfam13855
Location:790 → 850

LRR_8; Leucine rich repeat
NM_057381.4 → NP_476729.1 slit, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_476729.1

Status: REVIEWED

UniProtKB/TrEMBL

E1NZD5

Related

FBpp0303574

Conserved Domains (11) summary

smart00370
Location:813 → 836

LRR; Leucine-rich repeats, outliers

smart00013
Location:518 → 550

LRRNT; Leucine rich repeat N-terminal domain

smart00041
Location:1400 → 1469

CT; C-terminal cystine knot-like domain (CTCK)

smart00082
Location:653 → 702

LRRCT; Leucine rich repeat C-terminal domain

cd00054
Location:1064 → 1100

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...

cd00116
Location:550 → 654

LRR_RI; Leucine-rich repeats (LRRs), ribonuclease inhibitor (RI)-like subfamily. LRRs are 20-29 residue sequence motifs present in many proteins that participate in protein-protein interactions and have different functions and cellular locations. LRRs correspond ...

sd00033
Location:83 → 104

LRR_RI; leucine-rich repeat [structural motif]

pfam00008
Location:911 → 942

EGF; EGF-like domain

pfam00054
Location:1180 → 1311

Laminin_G_1; Laminin G domain

pfam12799
Location:347 → 387

LRR_4; Leucine Rich repeats (2 copies)

pfam13855
Location:766 → 826

LRR_8; Leucine rich repeat
NM_001103864.3 → NP_001097334.1 slit, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001097334.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A8DYF5, A8DYF6, P24014, Q24526, Q8MLB9, Q9V7F8, Q9V7F9, Q9XYV4

UniProtKB/TrEMBL

F0JAL2

Conserved Domains (11) summary

smart00369
Location:837 → 860

LRR_TYP; Leucine-rich repeats, typical (most populated) subfamily

smart00013
Location:542 → 574

LRRNT; Leucine rich repeat N-terminal domain

smart00041
Location:1435 → 1504

CT; C-terminal cystine knot-like domain (CTCK)

smart00082
Location:677 → 726

LRRCT; Leucine rich repeat C-terminal domain

cd00054
Location:1088 → 1124

EGF_CA; Calcium-binding EGF-like domain, present in a large number of membrane-bound and extracellular (mostly animal) proteins. Many of these proteins require calcium for their biological function and calcium-binding sites have been found to be located at the ...

cd00116
Location:119 → 259

LRR_RI; Leucine-rich repeats (LRRs), ribonuclease inhibitor (RI)-like subfamily. LRRs are 20-29 residue sequence motifs present in many proteins that participate in protein-protein interactions and have different functions and cellular locations. LRRs correspond ...

sd00033
Location:83 → 104

LRR_RI; leucine-rich repeat [structural motif]

pfam00008
Location:935 → 966

EGF; EGF-like domain

pfam00054
Location:1204 → 1335

Laminin_G_1; Laminin G domain

pfam12799
Location:371 → 411

LRR_4; Leucine Rich repeats (2 copies)

pfam13855
Location:790 → 850

LRR_8; Leucine rich repeat