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ana2 anastral spindle 2 [ Drosophila melanogaster (fruit fly) ]

Gene ID: 35878, updated on 4-Jan-2025

Summary

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Official Symbol
ana2provided by FlyBase
Official Full Name
anastral spindle 2provided by FlyBase
Primary source
FLYBASE:FBgn0027513
Locus tag
Dmel_CG8262
See related
AllianceGenome:FB:FBgn0027513
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Drosophila melanogaster
Lineage
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as
Ana-2; Ana2; BcDNA:LD22033; CG8262; Dmel\CG8262; dSTIL; STIL
Summary
Involved in asymmetric neuroblast division and microtubule cytoskeleton organization. Located in centriole; centrosome; and ciliary basal body. Is expressed in organism. [provided by Alliance of Genome Resources, Jan 2025]
Orthologs
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Genomic context

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See ana2 in Genome Data Viewer
Location:
44F3-44F3; 2-59 cM
Exon count:
2
Annotation release Status Assembly Chr Location
Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 2R NT_033778.4 (8898729..8900211, complement)
Release 5.57 previous assembly Release 5 (GCF_000001215.2) 2R NT_033778.3 (4786234..4787716, complement)

Chromosome 2R - NT_033778.4Genomic Context describing neighboring genes Neighboring gene Dynactin 2, p50 subunit Neighboring gene Secretory 31 Neighboring gene antisense RNA:CR45202 Neighboring gene uncharacterized protein Neighboring gene MRG/MORF4L binding protein Neighboring gene G protein gamma subunit 1

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies. Cottee MA, et al. Elife, 2015 May 23. PMID 26002084, Free PMC Article
  2. Generating CRISPR-edited clonal lines of cultured Drosophila S2 cells. Ryniawec JM, et al. Biol Methods Protoc, 2024. PMID 39206452, Free PMC Article
  3. The mechanism of dynein light chain LC8-mediated oligomerization of the Ana2 centriole duplication factor. Slevin LK, et al. J Biol Chem, 2014 Jul 25. PMID 24920673, Free PMC Article
  4. A key centriole assembly interaction interface between human PLK4 and STIL appears to not be conserved in flies. Cottee MA, et al. Biol Open, 2017 Mar 15. PMID 28202467, Free PMC Article
  5. Interaction interface in the C-terminal parts of centriole proteins Sas6 and Ana2. Fatalska A, et al. Open Biol, 2020 Nov. PMID 33171067, Free PMC Article

See all (80) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Centriole growth is limited by the Cdk/Cyclin-dependent phosphorylation of Ana2/STIL.
    Title: Centriole growth is limited by the Cdk/Cyclin-dependent phosphorylation of Ana2/STIL.
  2. SAPs as a new model to probe the pathway of centriole and centrosome assembly.
    Title: SAPs as a new model to probe the pathway of centriole and centrosome assembly.
  3. A molecular mechanism for the procentriole recruitment of Ana2.
    Title: A molecular mechanism for the procentriole recruitment of Ana2.
  4. Plk4 first phosphorylates the extreme N terminus of Ana2, which is critical for subsequent STAN domain modification. Phosphorylation of the central region then breaks the Plk4-Ana2 interaction. This phosphorylation pattern is important for centriole assembly and integrity.
    Title: An ordered pattern of Ana2 phosphorylation by Plk4 is required for centriole assembly.
  5. Two-step phosphorylation of Ana2 by Plk4 is required for Ana2 recruitment to the centriole.
    Title: Two-step phosphorylation of Ana2 by Plk4 is required for the sequential loading of Ana2 and Sas6 to initiate procentriole formation.
  6. Thus, efficient centriole duplication in flies requires the homo-oligomerisation of both Sas-6 and Ana2.
    Title: The homo-oligomerisation of both Sas-6 and Ana2 is required for efficient centriole assembly in flies.
  7. Size exclusion chromatography coupled with multiangle static light scattering demonstrates that LC8 dimers bind Ana2M sites and induce Ana2 tetramerization, yielding an Ana2M4-LC88 complex.
    Title: The mechanism of dynein light chain LC8-mediated oligomerization of the Ana2 centriole duplication factor.
  8. these findings reveal that the centrosomal proteins Ana2 and Ctp regulate Mud function to orient the mitotic spindle during neuroblasts asymmetric division.
    Title: An ana2/ctp/mud complex regulates spindle orientation in Drosophila neuroblasts.
  9. DSas-6 and Ana2 normally cooperate to drive the formation of the centriole inner cartwheel and they promote both centriole duplication and centriole cohesion in a Sak/Plk4-dependent manner.
    Title: DSas-6 and Ana2 coassemble into tubules to promote centriole duplication and engagement.
  10. Drosophila Ana2 is a conserved centriole duplication factor.
    Title: Drosophila Ana2 is a conserved centriole duplication factor.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by FlyBase

Process Evidence Code Pubs
involved_in asymmetric neuroblast division IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in centriole assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in centriole replication IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in centriole replication IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in centriole-centriole cohesion IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in ciliary basal body organization IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in ciliary basal body organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in establishment of mitotic spindle orientation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in microtubule anchoring at centrosome IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in centriole IDA
Inferred from Direct Assay
more info
 PubMed 
located_in centrosome IDA
Inferred from Direct Assay
more info
 PubMed 
located_in ciliary basal body IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
anastral spindle 2
Names
CG8262-PA
CG8262-PB
ana2-PA
ana2-PB
anastral spindle phenotype 2
anastral spindle-2

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NT_033778.4 Reference assembly

    Range
    8898729..8900211 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001299287.1 → NP_001286216.1  anastral spindle 2, isoform B [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001286216.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A0B4LFU9

  2. NM_136571.3 → NP_610415.1  anastral spindle 2, isoform A [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_610415.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    Q9XZ31
    Related
    FBpp0087766
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
A0A0B4LFU9 A0A0B4LFU9
Q9XZ31 Q9XZ31

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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