IKKepsilon I-kappaB kinase epsilon [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: IKKepsilonprovided by FlyBase
Official Full Name: I-kappaB kinase epsilonprovided by FlyBase
Primary source: FLYBASE:FBgn0086657
Locus tag: Dmel_CG2615
See related: AllianceGenome:FB:FBgn0086657
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: 38D.31; APTX7; CG2615; dik2; Dik2; DIK2; dIKK; dIKKepsilon; Dmel\CG2615; Dmik2; DmIKKepsilon; DmIKKepsilon/dIK2; dTBK1; ik2; Ik2; IK2; Ik2/DmIKK; IKK; ikkepsilon; l(2)38Ea
Summary: Enables protein serine/threonine kinase activity and small GTPase binding activity. Involved in several processes, including antiviral innate immune response; cell maturation; and cytoskeleton organization. Located in cell tip and cytoplasm. Human ortholog(s) of this gene implicated in brain disease and frontotemporal dementia and/or amyotrophic lateral sclerosis 4. Orthologous to several human genes including IKBKE (inhibitor of nuclear factor kappa B kinase subunit epsilon). [provided by Alliance of Genome Resources, Jan 2025]
Orthologs: all
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Genomic context

Location:: 38D4-38D5; 2-54 cM

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (20676045..20678979)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (20676045..20678979)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Inter-dependent Centrosomal Co-localization of the cen and ik2 cis-Natural Antisense mRNAs in Drosophila.
Title: Inter-dependent Centrosomal Co-localization of the cen and ik2 cis-Natural Antisense mRNAs in Drosophila.
Study found that Rab11, especially the active GTP-bound form, is required for dendrite pruning in Drosophila sensory neurons. Rab11 shows genetic interactions with spn-F and ik2 on pruning. Live imaging of single neurons and antibody staining reveal normal Ik2 kinase activation in Rab11 mutant neurons, suggesting that Rab11 could have a functional connection downstream of and/or parallel to the Ik2 kinase signaling.
Title: Rab11 activation by Ik2 kinase is required for dendrite pruning in Drosophila sensory neurons.
In glioma cell lines, the expression of IKKepsilon is suppressed by miR-98.
Title: Overexpression of miR-98 inhibits cell invasion in glioma cell lines via downregulation of IKKε.
findings reveal a novel regulatory mechanism for dendrite pruning achieved by temporal activation of Ik2 kinase and dynein-mediated redistribution of Ik2/Spn-F complex in neurons
Title: Spindle-F Is the Central Mediator of Ik2 Kinase-Dependent Dendrite Pruning in Drosophila Sensory Neurons.
These results suggest that polarized transport and selective retention regulate the distal tip localization of the Spn-F-IKKepsilon complex during bristle cell elongation.
Title: A transport and retention mechanism for the sustained distal localization of Spn-F-IKKε during Drosophila bristle elongation.
Spn-F mediates the interaction between Ik2 and Jvl to control Ik2 activity.
Title: Drosophila oocyte polarity and cytoskeleton organization require regulation of Ik2 activity by Spn-F and Javelin-like.
IKKepsilon: a kinase at the intersection of signaling and membrane traffic.
Title: IKKepsilon: a kinase at the intersection of signaling and membrane traffic.
These results establish an evolutionarily conserved function of IKK-related kinases in regulating recycling endosome dynamics and point to a key role of endosome dynamics in cell morphogenesis.
Title: IKKε regulates cell elongation through recycling endosome shuttling.
Spn-F and Ik2 are localized to the bristle tip and that mutations in ik2 and spn-F affect bristle microtubules and actin organization
Title: Asymmetric microtubule function is an essential requirement for polarized organization of the Drosophila bristle.
premature activation of Ik2 triggers precocious dendrite severing in larvae, revealing a critical role of Ik2 in initiating dendrite severing
Title: Drosophila IKK-related kinase Ik2 and Katanin p60-like 1 regulate dendrite pruning of sensory neuron during metamorphosis.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Dm0704 (e-PCR)
- UniSTS:71044

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables small GTPase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in actin filament bundle organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin filament organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in activation of innate immune response	IBA Inferred from Biological aspect of Ancestor more info
involved_in antiviral innate immune response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cGAS/STING signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chaeta morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endocytic recycling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in maintenance of epithelial integrity, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule bundle formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuron remodeling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oocyte anterior/posterior axis specification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of type I interferon production	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in regulation of cytoskeleton organization	IPI Inferred from Physical Interaction more info	PubMed
involved_in sensory organ precursor cell division	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cell tip	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: I-kappaB kinase epsilon

Names: CG2615-PB; CG2615-PC; IKK-related kinase; IKKepsilon-PB; IKKepsilon-PC; IkappaB kinase-like 2; TANK-binding kinase 1; apoptoxin 7; ik2; lethal (2) 38Ea

NP_610048.2

EC 2.7.11.1

NP_724278.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

20676045..20678979

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_136204.3 → NP_610048.2 I-kappaB kinase epsilon, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_610048.2

Status: REVIEWED

UniProtKB/TrEMBL

B5X521, Q7KJQ4, Q8INU8, Q9V3Y8

Conserved Domains (3) summary

cd12219
Location:299 → 389

UBL_TBK1_like; Ubiquitin-Like Domain Of Human Tbk1 and similar proteins

cd13988
Location:18 → 332

STKc_TBK1; Catalytic domain of the Serine/Threonine kinase, TANK Binding Kinase 1

pfam00069
Location:16 → 297

Pkinase; Protein kinase domain
NM_165337.2 → NP_724278.1 I-kappaB kinase epsilon, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_724278.1

Status: REVIEWED

UniProtKB/TrEMBL

B5X521, Q7KJQ4, Q8INU8, Q9V3Y8

Related

FBpp0080920

Conserved Domains (3) summary

cd12219
Location:299 → 389

UBL_TBK1_like; Ubiquitin-Like Domain Of Human Tbk1 and similar proteins

cd13988
Location:18 → 332

STKc_TBK1; Catalytic domain of the Serine/Threonine kinase, TANK Binding Kinase 1

pfam00069
Location:16 → 297

Pkinase; Protein kinase domain