LUBEL Linear Ubiquitin E3 ligase [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: LUBELprovided by FlyBase
Official Full Name: Linear Ubiquitin E3 ligaseprovided by FlyBase
Primary source: FLYBASE:FBgn0031857
Locus tag: Dmel_CG11321
See related: AllianceGenome:FB:FBgn0031857
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: AAF52435.2; CG11321; Dmel\CG11321; lubel; Lubel
Summary: Enables linear polyubiquitin binding activity; polyubiquitin modification-dependent protein binding activity; and ubiquitin protein ligase activity. Involved in several processes, including heat acclimation; positive regulation of peptidoglycan recognition protein signaling pathway; and protein linear polyubiquitination. Predicted to be part of LUBAC complex. Is expressed in dorsal pharyngeal muscle cell; larval somatic muscle cell; and somatic muscle primordium. Human ortholog(s) of this gene implicated in primary immunodeficiency disease. Orthologous to human RNF31 (ring finger protein 31). [provided by Alliance of Genome Resources, Jan 2025]
Orthologs: all
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Genomic context

Location:: 27B1-27B1; 2-22 cM

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (6759829..6772755, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (6759829..6772755, complement)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The linear ubiquitin E3 ligase-Relish pathway is involved in the regulation of proteostasis in Drosophila muscle during aging.
Title: The linear ubiquitin E3 ligase-Relish pathway is involved in the regulation of proteostasis in Drosophila muscle during aging.
This suggests that DIAP2 and LUBEL work together to promote Kenny-mediated activation of Relish..We found LUBEL-mediated M1-Ub chain formation to be required for flies to survive oral infection with Gram-negative bacteria, for activation of Relish-mediated expression of antimicrobial peptide genes and for pathogen clearance during oral infection
Title: M1-linked ubiquitination by LUBEL is required for inflammatory responses to oral infection in Drosophila.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables K48-linked polyubiquitin modification-dependent protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables K48-linked polyubiquitin modification-dependent protein binding	IDA Inferred from Direct Assay more info	PubMed
enables K63-linked polyubiquitin modification-dependent protein binding	IBA Inferred from Biological aspect of Ancestor more info
enables K63-linked polyubiquitin modification-dependent protein binding	IDA Inferred from Direct Assay more info	PubMed
enables linear polyubiquitin binding	IBA Inferred from Biological aspect of Ancestor more info
enables linear polyubiquitin binding	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin-protein transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin-protein transferase activity	ISS Inferred from Sequence or Structural Similarity more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	ISM Inferred from Sequence Model more info	PubMed

Process	Evidence Code	Pubs
involved_in defense response to Gram-negative bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heat acclimation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of peptidoglycan recognition protein signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in protein linear polyubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein linear polyubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein polyubiquitination	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
part_of LUBAC complex	IEA Inferred from Electronic Annotation more info
is_active_in cellular_component	ND No biological Data available more info

General protein information

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Preferred Names: linear ubiquitin E3 ligase

Names: CG11321-PC; CG11321-PD; CG11321-PE; CG11321-PF; CG11321-PG; CG11321-PH; CG11321-PI; CG11321-PJ; CG11321-PK; LUBEL-PC; LUBEL-PD; LUBEL-PE; LUBEL-PF; LUBEL-PG; LUBEL-PH; LUBEL-PI; LUBEL-PJ; LUBEL-PK

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

6759829..6772755 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001273228.2 → NP_001260157.1 linear ubiquitin E3 ligase, isoform G [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001260157.1

Status: REVIEWED

UniProtKB/TrEMBL

M9PB55

Conserved Domains (8) summary

pfam09528
Location:1298 → 1849

Ehrlichia_rpt; Ehrlichia tandem repeat (Ehrlichia_rpt)

smart00547
Location:737 → 761

ZnF_RBZ; Zinc finger domain

smart00647
Location:2557 → 2619

IBR; In Between Ring fingers

PRK01741
Location:1164 → 1361

PRK01741; cell division protein ZipA; Provisional

sd00029
Location:738 → 757

zf-RanBP2; RanBP2-type Zn finger [structural motif]

pfam01485
Location:2661 → 2683

IBR; IBR domain, a half RING-finger domain

pfam13923
Location:2473 → 2521

zf-C3HC4_2; Zinc finger, C3HC4 type (RING finger)

pfam16678
Location:1036 → 1177

HOIP-UBA; UB-associated-like domain of HOIP or E3 ubiquitin-protein ligase
NM_001273230.2 → NP_001260159.1 linear ubiquitin E3 ligase, isoform I [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001260159.1

Status: REVIEWED

UniProtKB/TrEMBL

M9PCS0

Conserved Domains (9) summary

pfam09528
Location:1286 → 1837

Ehrlichia_rpt; Ehrlichia tandem repeat (Ehrlichia_rpt)

smart00547
Location:684 → 708

ZnF_RBZ; Zinc finger domain

smart00647
Location:2545 → 2607

IBR; In Between Ring fingers

PRK01741
Location:1146 → 1349

PRK01741; cell division protein ZipA; Provisional

sd00029
Location:685 → 704

zf-RanBP2; RanBP2-type Zn finger [structural motif]

pfam01485
Location:2649 → 2671

IBR; IBR domain, a half RING-finger domain

pfam13923
Location:2461 → 2509

zf-C3HC4_2; Zinc finger, C3HC4 type (RING finger)

pfam16678
Location:983 → 1131

HOIP-UBA; UB-associated-like domain of HOIP or E3 ubiquitin-protein ligase

cl21463
Location:1076 → 1129

UBA_like_SF; UBA domain-like superfamily
NM_001273231.2 → NP_001260160.1 linear ubiquitin E3 ligase, isoform J [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001260160.1

Status: REVIEWED

UniProtKB/TrEMBL

M9PCD7

Conserved Domains (8) summary

pfam09528
Location:1245 → 1796

Ehrlichia_rpt; Ehrlichia tandem repeat (Ehrlichia_rpt)

smart00547
Location:684 → 708

ZnF_RBZ; Zinc finger domain

smart00647
Location:2504 → 2566

IBR; In Between Ring fingers

PRK01741
Location:1111 → 1308

PRK01741; cell division protein ZipA; Provisional

sd00029
Location:685 → 704

zf-RanBP2; RanBP2-type Zn finger [structural motif]

pfam01485
Location:2608 → 2630

IBR; IBR domain, a half RING-finger domain

pfam13923
Location:2420 → 2468

zf-C3HC4_2; Zinc finger, C3HC4 type (RING finger)

pfam16678
Location:983 → 1124

HOIP-UBA; UB-associated-like domain of HOIP or E3 ubiquitin-protein ligase
NM_164709.4 → NP_723214.2 linear ubiquitin E3 ligase, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_723214.2

Status: REVIEWED

UniProtKB/Swiss-Prot

M9PB55, M9PCD7, M9PCS0, Q8IPJ3, Q9VM88

Conserved Domains (12) summary

smart00547
Location:737 → 761

ZnF_RBZ; Zinc finger domain

smart00647
Location:2598 → 2660

IBR; In Between Ring fingers

PRK01741
Location:1199 → 1402

PRK01741; cell division protein ZipA; Provisional

PRK10811
Location:1482 → 1771

rne; ribonuclease E; Reviewed

sd00029
Location:738 → 757

zf-RanBP2; RanBP2-type Zn finger [structural motif]

pfam09606
Location:336 → 483

Med15; ARC105 or Med15 subunit of Mediator complex non-fungal

cd16551
Location:2513 → 2563

RING-HC_RNF169; RING finger, HC subclass, found in RING finger protein 169 (RNF169) and similar proteins

cd19815
Location:89 → 132

Bbox1_HOIP; B-box-type 1 zinc finger found in HOIL-1-interacting protein (HOIP) and similar proteins

pfam01485
Location:2690 → 2724

IBR; IBR domain, a half RING-finger domain

pfam16678
Location:1036 → 1184

HOIP-UBA; HOIP UBA domain pair

pfam18091
Location:2760 → 2858

E3_UbLigase_RBR; E3 Ubiquitin Ligase RBR C-terminal domain

NF033804
Location:2159 → 2307

Streccoc_I_II; antigen I/II family LPXTG-anchored adhesin
NM_001273229.2 → NP_001260158.1 linear ubiquitin E3 ligase, isoform H [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001260158.1

Status: REVIEWED

UniProtKB/TrEMBL

M9PC77

Conserved Domains (2) summary

smart00547
Location:737 → 761

ZnF_RBZ; Zinc finger domain

sd00029
Location:738 → 757

zf-RanBP2; RanBP2-type Zn finger [structural motif]
NM_001273227.1 → NP_001260156.1 linear ubiquitin E3 ligase, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001260156.1

Status: REVIEWED

UniProtKB/TrEMBL

Q9VM88

Conserved Domains (2) summary

smart00547
Location:684 → 708

ZnF_RBZ; Zinc finger domain

sd00029
Location:685 → 704

zf-RanBP2; RanBP2-type Zn finger [structural motif]
NM_135228.4 → NP_609072.2 linear ubiquitin E3 ligase, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_609072.2

Status: REVIEWED

UniProtKB/TrEMBL

Q9VM88

Conserved Domains (2) summary

smart00547
Location:684 → 708

ZnF_RBZ; Zinc finger domain

sd00029
Location:685 → 704

zf-RanBP2; RanBP2-type Zn finger [structural motif]
NM_001273232.2 → NP_001260161.1 linear ubiquitin E3 ligase, isoform K [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001260161.1

Status: REVIEWED

UniProtKB/TrEMBL

A9UN74, E1JHA3

Conserved Domains (1) summary

pfam01485
Location:60 → 123

IBR; IBR domain
NM_001169425.2 → NP_001162896.1 linear ubiquitin E3 ligase, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001162896.1

Status: REVIEWED

UniProtKB/TrEMBL

A9UN74, E1JHA3