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Gfat1 Glutamine:fructose-6-phosphate aminotransferase 1 [ Drosophila melanogaster (fruit fly) ]

Gene ID: 3354921, updated on 4-Jan-2025

Summary

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Official Symbol
Gfat1provided by FlyBase
Official Full Name
Glutamine:fructose-6-phosphate aminotransferase 1provided by FlyBase
Primary source
FLYBASE:FBgn0287209
Locus tag
Dmel_CG12449
See related
AllianceGenome:FB:FBgn0287209
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Drosophila melanogaster
Lineage
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as
CG12449; CG40197; Dmel/Gfat1; Dmel\CG12449; gfat; Gfat; GFAT; gfat1; zep
Summary
Enables glutamine-fructose-6-phosphate transaminase (isomerizing) activity. Involved in UDP-N-acetylglucosamine metabolic process. Located in cytoplasm. Is expressed in several structures, including adult fat body; adult head; embryonic head epidermis; epidermis; and gland. Human ortholog(s) of this gene implicated in congenital myasthenic syndrome 12; obesity; and type 2 diabetes mellitus. Orthologous to several human genes including GFPT2 (glutamine-fructose-6-phosphate transaminase 2). [provided by Alliance of Genome Resources, Jan 2025]
Orthologs
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Genomic context

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See Gfat1 in Genome Data Viewer
Location:
81F5-81F5; 3-47.1 cM
Exon count:
11
Annotation release Status Assembly Chr Location
Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 3R NT_033777.3 (4126442..4137882)
Release 5.57 previous assembly Release 5 (GCF_000001215.2) 3RHet NW_001848858.1 (2470208..2481648)

Chromosome 3R - NT_033777.3Genomic Context describing neighboring genes Neighboring gene uncharacterized protein Neighboring gene uncharacterized protein Neighboring gene long non-coding RNA:CR41601 Neighboring gene uncharacterized protein

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Detection of frame-shifts within homopolymeric DNA tracts using the amplification refractory mutation system (ARMS). Graack HR, et al. Biotechniques, 1999 Oct. PMID 10524301
  2. Functional regulation of glutamine:fructose-6-phosphate aminotransferase 1 (GFAT1) of Drosophila melanogaster in a UDP-N-acetylglucosamine and cAMP-dependent manner. Graack HR, et al. Biochem J, 2001 Dec 1. PMID 11716769, Free PMC Article
  3. Drosophila GFAT1 and GFAT2 enzymes encode obligate developmental functions. Chen P, et al. Fly (Austin), 2020 Mar-Dec. PMID 32615907, Free PMC Article
  4. Regulation of protein O-GlcNAcylation by circadian, metabolic, and cellular signals. Liu X, et al. J Biol Chem, 2024 Feb. PMID 38159854, Free PMC Article
  5. New local potential useful for genome annotation and 3D modeling. Chandonia JM, et al. J Mol Biol, 2003 Sep 26. PMID 12972255

See all (50) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Drosophila GFAT1 and GFAT2 enzymes encode obligate developmental functions.
    Title: Drosophila GFAT1 and GFAT2 enzymes encode obligate developmental functions.
  2. By whole-mount in situ hybridization, specific expression of GFAT1 is detected in embryonic chitin-synthesizing tissues and in the corpus cells of salivary glands from late third larval instar.
    Title: Functional regulation of glutamine:fructose-6-phosphate aminotransferase 1 (GFAT1) of Drosophila melanogaster in a UDP-N-acetylglucosamine and cAMP-dependent manner.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

  • Annotated by Drosophila Heterochromatin Genome Project, Lawrence Berkeley National Lab, http://www.dhgp.org

Gene Ontology Provided by FlyBase

Function Evidence Code Pubs
enables carbohydrate derivative binding IEA
Inferred from Electronic Annotation
more info
  
enables glutamine-fructose-6-phosphate transaminase (isomerizing) activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables glutamine-fructose-6-phosphate transaminase (isomerizing) activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables glutamine-fructose-6-phosphate transaminase (isomerizing) activity IEA
Inferred from Electronic Annotation
more info
  
enables glutamine-fructose-6-phosphate transaminase (isomerizing) activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in UDP-N-acetylglucosamine metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in UDP-N-acetylglucosamine metabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in carbohydrate derivative biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in fructose 6-phosphate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in fructose 6-phosphate metabolic process TAS
Traceable Author Statement
more info
 PubMed 
involved_in protein N-linked glycosylation IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
Glutamine:fructose-6-phosphate aminotransferase 1
Names
CG12449-PA
CG12449-PD
CG12449-PF
CG12449-PH
CG12449-PJ
CG12449-PK
CG12449-PL
CG12449-PM
CG12449-PN
Gfat1-PA
Gfat1-PD
Gfat1-PF
Gfat1-PH
Gfat1-PJ
Gfat1-PK
Gfat1-PL
Gfat1-PM
Gfat1-PN
Glutamine: Fructose-6-Phosphate aminotransferase 1
glucose-fructose amidotransferase 1
zeppelin
NP_001015157.2
NP_001015158.1
NP_001015159.2
NP_001015160.2
NP_001015161.2
NP_001015162.1
NP_001104465.1
NP_001188368.1
NP_001188369.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NT_033777.3 Reference assembly

    Range
    4126442..4137882
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001201440.1 → NP_001188369.1  Glutamine:fructose-6-phosphate aminotransferase 1, isoform N [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001188369.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    Q7PLC7, Q9Y093
    Conserved Domains (4) summary
    PLN02981
    Location:1 → 694
    PLN02981; glucosamine:fructose-6-phosphate aminotransferase
    cd00714
    Location:2 → 297
    GFAT; Glutamine amidotransferases class-II (Gn-AT)_GFAT-type. This domain is found at the N-terminus of glucosamine-6P synthase (GlmS, or GFAT in humans). The glutaminase domain catalyzes amide nitrogen transfer from glutamine to the appropriate substrate. In ...
    cd05008
    Location:381 → 506
    SIS_GlmS_GlmD_1; SIS (Sugar ISomerase) domain repeat 1 found in Glucosamine 6-phosphate synthase (GlmS) and Glucosamine-6-phosphate deaminase (GlmD). The SIS domain is found in many phosphosugar isomerases and phosphosugar binding proteins. GlmS contains a N-terminal ...
    cd05009
    Location:539 → 692
    SIS_GlmS_GlmD_2; SIS (Sugar ISomerase) domain repeat 2 found in Glucosamine 6-phosphate synthase (GlmS) and Glucosamine-6-phosphate deaminase (GlmD). The SIS domain is found in many phosphosugar isomerases and phosphosugar binding proteins. GlmS contains a N-terminal ...

  2. NM_001110995.2 → NP_001104465.1  Glutamine:fructose-6-phosphate aminotransferase 1, isoform H [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001104465.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A8Y596, Q9Y093
    Conserved Domains (4) summary
    PLN02981
    Location:1 → 685
    PLN02981; glucosamine:fructose-6-phosphate aminotransferase
    cd00714
    Location:2 → 288
    GFAT; Glutamine amidotransferases class-II (Gn-AT)_GFAT-type. This domain is found at the N-terminus of glucosamine-6P synthase (GlmS, or GFAT in humans). The glutaminase domain catalyzes amide nitrogen transfer from glutamine to the appropriate substrate. In ...
    cd05008
    Location:372 → 497
    SIS_GlmS_GlmD_1; SIS (Sugar ISomerase) domain repeat 1 found in Glucosamine 6-phosphate synthase (GlmS) and Glucosamine-6-phosphate deaminase (GlmD). The SIS domain is found in many phosphosugar isomerases and phosphosugar binding proteins. GlmS contains a N-terminal ...
    cd05009
    Location:530 → 683
    SIS_GlmS_GlmD_2; SIS (Sugar ISomerase) domain repeat 2 found in Glucosamine 6-phosphate synthase (GlmS) and Glucosamine-6-phosphate deaminase (GlmD). The SIS domain is found in many phosphosugar isomerases and phosphosugar binding proteins. GlmS contains a N-terminal ...

  3. NM_001201439.2 → NP_001188368.1  Glutamine:fructose-6-phosphate aminotransferase 1, isoform J [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001188368.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    E8NHS9, Q9Y093
    Conserved Domains (4) summary
    PLN02981
    Location:2 → 643
    PLN02981; glucosamine:fructose-6-phosphate aminotransferase
    cd00714
    Location:1 → 246
    GFAT; Glutamine amidotransferases class-II (Gn-AT)_GFAT-type. This domain is found at the N-terminus of glucosamine-6P synthase (GlmS, or GFAT in humans). The glutaminase domain catalyzes amide nitrogen transfer from glutamine to the appropriate substrate. In ...
    cd05008
    Location:330 → 455
    SIS_GlmS_GlmD_1; SIS (Sugar ISomerase) domain repeat 1 found in Glucosamine 6-phosphate synthase (GlmS) and Glucosamine-6-phosphate deaminase (GlmD). The SIS domain is found in many phosphosugar isomerases and phosphosugar binding proteins. GlmS contains a N-terminal ...
    cd05009
    Location:488 → 641
    SIS_GlmS_GlmD_2; SIS (Sugar ISomerase) domain repeat 2 found in Glucosamine 6-phosphate synthase (GlmS) and Glucosamine-6-phosphate deaminase (GlmD). The SIS domain is found in many phosphosugar isomerases and phosphosugar binding proteins. GlmS contains a N-terminal ...

  4. NM_001015159.4 → NP_001015159.2  Glutamine:fructose-6-phosphate aminotransferase 1, isoform K [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001015159.2

    Status: REVIEWED

    UniProtKB/TrEMBL
    C3KGM9, Q7PLC5
    Conserved Domains (4) summary
    COG2222
    Location:137 → 493
    AgaS; Fructoselysine-6-P-deglycase FrlB and related proteins with duplicated sugar isomerase (SIS) domain [Cell wall/membrane/envelope biogenesis]
    cd05008
    Location:180 → 305
    SIS_GlmS_GlmD_1; SIS (Sugar ISomerase) domain repeat 1 found in Glucosamine 6-phosphate synthase (GlmS) and Glucosamine-6-phosphate deaminase (GlmD). The SIS domain is found in many phosphosugar isomerases and phosphosugar binding proteins. GlmS contains a N-terminal ...
    cd05009
    Location:338 → 491
    SIS_GlmS_GlmD_2; SIS (Sugar ISomerase) domain repeat 2 found in Glucosamine 6-phosphate synthase (GlmS) and Glucosamine-6-phosphate deaminase (GlmD). The SIS domain is found in many phosphosugar isomerases and phosphosugar binding proteins. GlmS contains a N-terminal ...
    cl00319
    Location:65 → 96
    Gn_AT_II; Glutamine amidotransferases class-II (GATase). The glutaminase domain catalyzes an amide nitrogen transfer from glutamine to the appropriate substrate. In this process, glutamine is hydrolyzed to glutamic acid and ammonia. This domain is related to ...

  5. NM_001015157.4 → NP_001015157.2  Glutamine:fructose-6-phosphate aminotransferase 1, isoform A [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001015157.2

    Status: REVIEWED

    UniProtKB/TrEMBL
    Q7PLC7, Q9Y093
    Conserved Domains (4) summary
    PLN02981
    Location:1 → 694
    PLN02981; glucosamine:fructose-6-phosphate aminotransferase
    cd00714
    Location:2 → 297
    GFAT; Glutamine amidotransferases class-II (Gn-AT)_GFAT-type. This domain is found at the N-terminus of glucosamine-6P synthase (GlmS, or GFAT in humans). The glutaminase domain catalyzes amide nitrogen transfer from glutamine to the appropriate substrate. In ...
    cd05008
    Location:381 → 506
    SIS_GlmS_GlmD_1; SIS (Sugar ISomerase) domain repeat 1 found in Glucosamine 6-phosphate synthase (GlmS) and Glucosamine-6-phosphate deaminase (GlmD). The SIS domain is found in many phosphosugar isomerases and phosphosugar binding proteins. GlmS contains a N-terminal ...
    cd05009
    Location:539 → 692
    SIS_GlmS_GlmD_2; SIS (Sugar ISomerase) domain repeat 2 found in Glucosamine 6-phosphate synthase (GlmS) and Glucosamine-6-phosphate deaminase (GlmD). The SIS domain is found in many phosphosugar isomerases and phosphosugar binding proteins. GlmS contains a N-terminal ...

  6. NM_001015160.4 → NP_001015160.2  Glutamine:fructose-6-phosphate aminotransferase 1, isoform L [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001015160.2

    Status: REVIEWED

    UniProtKB/TrEMBL
    A8Y599, Q9Y093
    Conserved Domains (4) summary
    PLN02981
    Location:1 → 682
    PLN02981; glucosamine:fructose-6-phosphate aminotransferase
    cd00714
    Location:2 → 285
    GFAT; Glutamine amidotransferases class-II (Gn-AT)_GFAT-type. This domain is found at the N-terminus of glucosamine-6P synthase (GlmS, or GFAT in humans). The glutaminase domain catalyzes amide nitrogen transfer from glutamine to the appropriate substrate. In ...
    cd05008
    Location:369 → 494
    SIS_GlmS_GlmD_1; SIS (Sugar ISomerase) domain repeat 1 found in Glucosamine 6-phosphate synthase (GlmS) and Glucosamine-6-phosphate deaminase (GlmD). The SIS domain is found in many phosphosugar isomerases and phosphosugar binding proteins. GlmS contains a N-terminal ...
    cd05009
    Location:527 → 680
    SIS_GlmS_GlmD_2; SIS (Sugar ISomerase) domain repeat 2 found in Glucosamine 6-phosphate synthase (GlmS) and Glucosamine-6-phosphate deaminase (GlmD). The SIS domain is found in many phosphosugar isomerases and phosphosugar binding proteins. GlmS contains a N-terminal ...

  7. NM_001015158.4 → NP_001015158.1  Glutamine:fructose-6-phosphate aminotransferase 1, isoform D [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001015158.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    Q7PLC6, Q9Y093
    Conserved Domains (4) summary
    PLN02981
    Location:1 → 673
    PLN02981; glucosamine:fructose-6-phosphate aminotransferase
    cd00714
    Location:2 → 276
    GFAT; Glutamine amidotransferases class-II (Gn-AT)_GFAT-type. This domain is found at the N-terminus of glucosamine-6P synthase (GlmS, or GFAT in humans). The glutaminase domain catalyzes amide nitrogen transfer from glutamine to the appropriate substrate. In ...
    cd05008
    Location:360 → 485
    SIS_GlmS_GlmD_1; SIS (Sugar ISomerase) domain repeat 1 found in Glucosamine 6-phosphate synthase (GlmS) and Glucosamine-6-phosphate deaminase (GlmD). The SIS domain is found in many phosphosugar isomerases and phosphosugar binding proteins. GlmS contains a N-terminal ...
    cd05009
    Location:518 → 671
    SIS_GlmS_GlmD_2; SIS (Sugar ISomerase) domain repeat 2 found in Glucosamine 6-phosphate synthase (GlmS) and Glucosamine-6-phosphate deaminase (GlmD). The SIS domain is found in many phosphosugar isomerases and phosphosugar binding proteins. GlmS contains a N-terminal ...

  8. NM_001015161.4 → NP_001015161.2  Glutamine:fructose-6-phosphate aminotransferase 1, isoform M [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001015161.2

    Status: REVIEWED

    UniProtKB/TrEMBL
    A8Y5A0, Q9Y093
    Conserved Domains (4) summary
    PLN02981
    Location:1 → 665
    PLN02981; glucosamine:fructose-6-phosphate aminotransferase
    cd00714
    Location:2 → 268
    GFAT; Glutamine amidotransferases class-II (Gn-AT)_GFAT-type. This domain is found at the N-terminus of glucosamine-6P synthase (GlmS, or GFAT in humans). The glutaminase domain catalyzes amide nitrogen transfer from glutamine to the appropriate substrate. In ...
    cd05008
    Location:352 → 477
    SIS_GlmS_GlmD_1; SIS (Sugar ISomerase) domain repeat 1 found in Glucosamine 6-phosphate synthase (GlmS) and Glucosamine-6-phosphate deaminase (GlmD). The SIS domain is found in many phosphosugar isomerases and phosphosugar binding proteins. GlmS contains a N-terminal ...
    cd05009
    Location:510 → 663
    SIS_GlmS_GlmD_2; SIS (Sugar ISomerase) domain repeat 2 found in Glucosamine 6-phosphate synthase (GlmS) and Glucosamine-6-phosphate deaminase (GlmD). The SIS domain is found in many phosphosugar isomerases and phosphosugar binding proteins. GlmS contains a N-terminal ...

  9. NM_001015162.4 → NP_001015162.1  Glutamine:fructose-6-phosphate aminotransferase 1, isoform F [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001015162.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    A8Y5A1
    Conserved Domains (4) summary
    COG2222
    Location:78 → 434
    AgaS; Fructoselysine-6-P-deglycase FrlB and related proteins with duplicated sugar isomerase (SIS) domain [Cell wall/membrane/envelope biogenesis]
    cd05008
    Location:121 → 246
    SIS_GlmS_GlmD_1; SIS (Sugar ISomerase) domain repeat 1 found in Glucosamine 6-phosphate synthase (GlmS) and Glucosamine-6-phosphate deaminase (GlmD). The SIS domain is found in many phosphosugar isomerases and phosphosugar binding proteins. GlmS contains a N-terminal ...
    cd05009
    Location:279 → 432
    SIS_GlmS_GlmD_2; SIS (Sugar ISomerase) domain repeat 2 found in Glucosamine 6-phosphate synthase (GlmS) and Glucosamine-6-phosphate deaminase (GlmD). The SIS domain is found in many phosphosugar isomerases and phosphosugar binding proteins. GlmS contains a N-terminal ...
    cl00319
    Location:6 → 37
    Gn_AT_II; Glutamine amidotransferases class-II (GATase). The glutaminase domain catalyzes an amide nitrogen transfer from glutamine to the appropriate substrate. In this process, glutamine is hydrolyzed to glutamic acid and ammonia. This domain is related to ...
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
A8Y596 A8Y596
A8Y599 A8Y599
A8Y5A0 A8Y5A0
A8Y5A1 A8Y5A1
E8NHS9 E8NHS9
Q7PLC5 Q7PLC5
Q7PLC6 Q7PLC6
Q7PLC7 Q7PLC7
Q9Y093 Q9Y093

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