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Pcmt1 protein-L-isoaspartate (D-aspartate) O-methyltransferase 1 [ Rattus norvegicus (Norway rat) ]

Gene ID: 25604, updated on 27-Nov-2024

Summary

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Official Symbol
Pcmt1provided by RGD
Official Full Name
protein-L-isoaspartate (D-aspartate) O-methyltransferase 1provided by RGD
Primary source
RGD:3268
See related
EnsemblRapid:ENSRNOG00000014330 AllianceGenome:RGD:3268
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as
PCM
Summary
Enables protein-L-isoaspartate (D-aspartate) O-methyltransferase activity. Involved in several processes, including S-adenosylhomocysteine metabolic process; cellular response to hypoxia; and negative regulation of cardiac muscle cell apoptotic process. Located in several cellular components, including basolateral plasma membrane; brush border membrane; and perikaryon. Biomarker of high grade glioma. Orthologous to human PCMT1 (protein-L-isoaspartate (D-aspartate) O-methyltransferase). [provided by Alliance of Genome Resources, Nov 2024]
Expression
Biased expression in Brain (RPKM 550.2), Heart (RPKM 539.4) and 9 other tissues See more
Orthologs
human mouse all
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Genomic context

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See Pcmt1 in Genome Data Viewer
Location:
1p13
Exon count:
11
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) 1 NC_086019.1 (3932161..3980751, complement)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 1 NC_051336.1 (2111756..2160354, complement)
106 previous assembly Rnor_6.0 (GCF_000001895.5) 1 NC_005100.4 (1734863..1767759, complement)

Chromosome 1 - NC_086019.1Genomic Context describing neighboring genes Neighboring gene ribosomal protein L23a, pseudogene 6 Neighboring gene uncharacterized LOC120098770 Neighboring gene LDL receptor related protein 11 Neighboring gene nucleoporin 43 Neighboring gene large tumor suppressor kinase 1 Neighboring gene small ribosomal subunit protein uS12-like

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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Related articles in PubMed

  1. Accumulation of proteins bearing atypical isoaspartyl residues in livers of alcohol-fed rats is prevented by betaine administration: effects on protein-L-isoaspartyl methyltransferase activity. Kharbanda KK, et al. J Hepatol, 2007 Jun. PMID 17336420
  2. Embryonic genes expressed in Alzheimer's disease brains. Kondo T, et al. Neurosci Lett, 1996 May 17. PMID 8736634
  3. Detection and characterization of a protein isoaspartyl methyltransferase which becomes trapped in the extracellular space during blood vessel injury. Weber DJ, et al. J Protein Chem, 1997 May. PMID 9188065
  4. Tissue-specific expression of isoaspartyl protein carboxyl methyltransferase gene in rat brain and testis. Mizobuchi M, et al. J Neurochem, 1994 Jan. PMID 8263531
  5. Protein L-isoaspartyl methyltransferase: developmentally regulated gene expression and protein localization in the central nervous system of aged rat. Shirasawa T, et al. Neurosci Lett, 1995 Mar 16. PMID 7783974

See all (22) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. PIMT was critical for bFGF-mediated neuronal differentiation of PC12 cells and regulated the MAPK and Akt signaling pathways.
    Title: Critical role of protein L-isoaspartyl methyltransferase in basic fibroblast growth factor-mediated neuronal cell differentiation.
  2. ERK2-mediated phosphorylation of transcriptional coactivator binding protein PIMT/NCoA6IP at Ser298 augments hepatic gluconeogenesis.
    Title: ERK2-mediated phosphorylation of transcriptional coactivator binding protein PIMT/NCoA6IP at Ser298 augments hepatic gluconeogenesis.
  3. isoforms of the PIMT/Tgs1 protein with an RNA methyltransferase domain function both in the nucleus and in the cytoplasm
    Title: Different isoforms of PRIP-interacting protein with methyltransferase domain/trimethylguanosine synthase localizes to the cytoplasm and nucleus.
  4. PIMT-KO phenotype results from the cumulative effect of isoaspartate-related damage to a number of the neuron-rich proteins detected in this study
    Title: Protein repair in the brain, proteomic analysis of endogenous substrates for protein L-isoaspartyl methyltransferase in mouse brain.
  5. Ethanol consumption results in the accumulation of modified proteins bearing atypical isoaspartyl residues via impaired in vivo PIMT activity, while betaine administration prevents it by restoring the PIMT-catalyzed protein repair reaction
    Title: Accumulation of proteins bearing atypical isoaspartyl residues in livers of alcohol-fed rats is prevented by betaine administration: effects on protein-L-isoaspartyl methyltransferase activity.
Submit: New GeneRIF Correction

Pathways from PubChem

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General gene information

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Markers

Homology

Clone Names

  • MGC95039

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables S-adenosylmethionine-dependent methyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein-L-isoaspartate (D-aspartate) O-methyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein-L-isoaspartate (D-aspartate) O-methyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein-L-isoaspartate (D-aspartate) O-methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein-L-isoaspartate (D-aspartate) O-methyltransferase activity ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in S-adenosylhomocysteine metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in S-adenosylmethionine metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in cellular response to hypoxia IEP
Inferred from Expression Pattern
more info
 PubMed 
involved_in methylation IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of cardiac muscle cell apoptotic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein methylation IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within protein methylation ISO
Inferred from Sequence Orthology
more info
  
involved_in protein methylation ISO
Inferred from Sequence Orthology
more info
  
involved_in protein modification process IEA
Inferred from Electronic Annotation
more info
  
involved_in response to ethanol IEP
Inferred from Expression Pattern
more info
 PubMed 
Component Evidence Code Pubs
located_in basolateral plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in brush border membrane IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in extracellular space IDA
Inferred from Direct Assay
more info
 PubMed 
located_in perikaryon IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
protein-L-isoaspartate(D-aspartate) O-methyltransferase
Names
L-isoaspartyl protein carboxyl methyltransferase
PIMT
protein L-isoaspartyl/D-aspartyl methyltransferase
protein-beta-aspartate methyltransferase
NP_001385490.1
NP_001385491.1
NP_037205.3
XP_038957965.1
XP_038957986.1
XP_063138121.1
XP_063138126.1
XP_063138131.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001398561.1 → NP_001385490.1  protein-L-isoaspartate(D-aspartate) O-methyltransferase isoform a

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000001
    Conserved Domains (1) summary
    pfam01135
    Location:66 → 274
    PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)

  2. NM_001398562.1 → NP_001385491.1  protein-L-isoaspartate(D-aspartate) O-methyltransferase isoform c

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000001
    Related
    ENSRNOP00000108494.1, ENSRNOT00000150113.1
    Conserved Domains (1) summary
    pfam01135
    Location:66 → 226
    PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)

  3. NM_013073.4 → NP_037205.3  protein-L-isoaspartate(D-aspartate) O-methyltransferase isoform b

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000001
    UniProtKB/TrEMBL
    A0A140TAB9
    Related
    ENSRNOP00000019623.8, ENSRNOT00000019623.9
    Conserved Domains (1) summary
    pfam01135
    Location:66 → 274
    PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086019.1 Reference GRCr8

    Range
    3932161..3980751 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_039102058.2 → XP_038957986.1  protein-L-isoaspartate(D-aspartate) O-methyltransferase isoform X3

    UniProtKB/TrEMBL
    A6JP11, A6JP13
    Conserved Domains (1) summary
    pfam01135
    Location:1 → 185
    PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)

  2. XM_063282051.1 → XP_063138121.1  protein-L-isoaspartate(D-aspartate) O-methyltransferase isoform X2

  3. XM_039102037.2 → XP_038957965.1  protein-L-isoaspartate(D-aspartate) O-methyltransferase isoform X1

    UniProtKB/TrEMBL
    A6JP10
    Related
    ENSRNOP00000103701.1, ENSRNOT00000123595.1
    Conserved Domains (1) summary
    pfam01135
    Location:77 → 239
    PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)

  4. XM_063282056.1 → XP_063138126.1  protein-L-isoaspartate(D-aspartate) O-methyltransferase isoform X4

  5. XM_063282061.1 → XP_063138131.1  protein-L-isoaspartate(D-aspartate) O-methyltransferase isoform X5

Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P22062.2 GenPept UniProtKB/Swiss-Prot:P22062

Gene LinkOut

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