Arhgap4 Rho GTPase activating protein 4 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Arhgap4provided by RGD
Official Full Name: Rho GTPase activating protein 4provided by RGD
Primary source: RGD:628901
See related: EnsemblRapid:ENSRNOG00000058545 AllianceGenome:RGD:628901
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables GTPase activator activity. Involved in negative regulation of axon extension; negative regulation of fibroblast migration; and nervous system development. Located in growth cone and microtubule. Orthologous to human ARHGAP4 (Rho GTPase activating protein 4). [provided by Alliance of Genome Resources, Jan 2025]
Expression: Biased expression in Thymus (RPKM 229.2), Spleen (RPKM 224.7) and 8 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: Xq37

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	X	NC_086039.1 (156787566..156802841, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	X	NC_051356.1 (151636071..151651528, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	X	NC_005120.4 (156873094..156888762)

Chromosome X - NC_086039.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

These observations suggest that ARHGAP4 can act as a potent inhibitor of cell and axon motility when it is localized to the leading edge of motile cells and axons.
Title: ARHGAP4 is a novel RhoGAP that mediates inhibition of cell motility and axon outgrowth.
ARHGAP4 occured in the hippocampus, ventral region of the brainstem and striatum, and in cerebellar granule cells. Subcellularly, ARHGAP4 localized to the Golgi complex and could redistribute to the microtubules, for example during mitosis.
Title: Cloning of rat ARHGAP4/C1, a RhoGAP family member expressed in the nervous system that colocalizes with the Golgi complex and microtubules.

Submit: New GeneRIF Correction

General gene information

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Markers

RH127646 (e-PCR)
- UniSTS:210956

AI028822 (e-PCR)
- UniSTS:226209

REN88106 (e-PCR)
- UniSTS:412904

GDB:182522 (e-PCR)
- UniSTS:155385

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables GTPase activator activity	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in negative regulation of axon extension	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cell migration	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of fibroblast migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nervous system development	IEP Inferred from Expression Pattern more info	PubMed
involved_in regulation of Rho protein signal transduction	NAS Non-traceable Author Statement more info	PubMed
involved_in signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in growth cone	IDA Inferred from Direct Assay more info	PubMed
located_in microtubule	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: rho GTPase-activating protein 4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_144740.3 → NP_653341.2 rho GTPase-activating protein 4

See identical proteins and their annotated locations for NP_653341.2

Status: VALIDATED

Source sequence(s)

AF401635, CO572120

UniProtKB/TrEMBL

A0A0G2JVF0, A6KRT9, Q8K4G7

Related

ENSRNOP00000069460.2, ENSRNOT00000085410.3

Conserved Domains (3) summary

cd07656
Location:27 → 299

F-BAR_srGAP; The F-BAR (FES-CIP4 Homology and Bin/Amphiphysin/Rvs) domain of Slit-Robo GTPase Activating Proteins

cl02570
Location:522 → 709

RhoGAP; RhoGAP: GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when ...

cl17036
Location:767 → 821

SH3; Src Homology 3 domain superfamily

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086039.1 Reference GRCr8

Range

156787566..156802841 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039099454.2 → XP_038955382.1 rho GTPase-activating protein 4 isoform X5

UniProtKB/TrEMBL

Q8K4G7

Conserved Domains (3) summary

cd07656
Location:27 → 299

F-BAR_srGAP; The F-BAR (FES-CIP4 Homology and Bin/Amphiphysin/Rvs) domain of Slit-Robo GTPase Activating Proteins

cl02570
Location:488 → 675

RhoGAP; GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when bound to ...

cl17036
Location:739 → 793

SH3; Src Homology 3 domain superfamily
XM_039099456.2 → XP_038955384.1 rho GTPase-activating protein 4 isoform X8

UniProtKB/TrEMBL

Q8K4G7

Conserved Domains (3) summary

cd07656
Location:27 → 299

F-BAR_srGAP; The F-BAR (FES-CIP4 Homology and Bin/Amphiphysin/Rvs) domain of Slit-Robo GTPase Activating Proteins

cl02570
Location:522 → 659

RhoGAP; GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when bound to ...

cl17036
Location:695 → 749

SH3; Src Homology 3 domain superfamily
XM_039099451.2 → XP_038955379.1 rho GTPase-activating protein 4 isoform X1

UniProtKB/TrEMBL

Q8K4G7

Conserved Domains (3) summary

cd07656
Location:27 → 299

F-BAR_srGAP; The F-BAR (FES-CIP4 Homology and Bin/Amphiphysin/Rvs) domain of Slit-Robo GTPase Activating Proteins

cl02570
Location:522 → 709

RhoGAP; GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when bound to ...

cl17036
Location:773 → 827

SH3; Src Homology 3 domain superfamily
XM_039099453.2 → XP_038955381.1 rho GTPase-activating protein 4 isoform X4

UniProtKB/TrEMBL

Q8K4G7

Conserved Domains (3) summary

cl02570
Location:491 → 678

RhoGAP; GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when bound to ...

cl12013
Location:27 → 268

BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature

cl17036
Location:742 → 796

SH3; Src Homology 3 domain superfamily
XM_039099457.2 → XP_038955385.1 rho GTPase-activating protein 4 isoform X9

UniProtKB/TrEMBL

Q8K4G7

Conserved Domains (3) summary

cd07656
Location:27 → 299

F-BAR_srGAP; The F-BAR (FES-CIP4 Homology and Bin/Amphiphysin/Rvs) domain of Slit-Robo GTPase Activating Proteins

cl02570
Location:522 → 659

RhoGAP; GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when bound to ...

cl17036
Location:689 → 743

SH3; Src Homology 3 domain superfamily
XM_039099452.2 → XP_038955380.1 rho GTPase-activating protein 4 isoform X2

UniProtKB/TrEMBL

Q8K4G7

Conserved Domains (3) summary

cd07656
Location:27 → 289

F-BAR_srGAP; The F-BAR (FES-CIP4 Homology and Bin/Amphiphysin/Rvs) domain of Slit-Robo GTPase Activating Proteins

cl02570
Location:512 → 699

RhoGAP; GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when bound to ...

cl17036
Location:763 → 817

SH3; Src Homology 3 domain superfamily
XM_063279779.1 → XP_063135849.1 rho GTPase-activating protein 4 isoform X10

UniProtKB/TrEMBL

Q8K4G7

Related

ENSRNOP00000095360.2, ENSRNOT00000117215.2
XM_006229546.5 → XP_006229608.1 rho GTPase-activating protein 4 isoform X6

See identical proteins and their annotated locations for XP_006229608.1

UniProtKB/TrEMBL

A0A8I6AIK5, A6KRU1, Q8K4G7

Related

ENSRNOP00000094863.1, ENSRNOT00000109725.2

Conserved Domains (3) summary

cl02570
Location:491 → 678

RhoGAP; RhoGAP: GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when ...

cl12013
Location:27 → 268

BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature

cl17036
Location:736 → 790

SH3; Src Homology 3 domain superfamily
XM_063279778.1 → XP_063135848.1 rho GTPase-activating protein 4 isoform X7

UniProtKB/TrEMBL

A0A8I5ZY52, Q8K4G7

Related

ENSRNOP00000083983.1, ENSRNOT00000109822.2
XM_006229545.5 → XP_006229607.1 rho GTPase-activating protein 4 isoform X3

See identical proteins and their annotated locations for XP_006229607.1

UniProtKB/TrEMBL

A6KRU0, Q8K4G7

Related

ENSRNOP00000091062.1, ENSRNOT00000104884.2

Conserved Domains (3) summary

cd07656
Location:27 → 289

F-BAR_srGAP; The F-BAR (FES-CIP4 Homology and Bin/Amphiphysin/Rvs) domain of Slit-Robo GTPase Activating Proteins

cl02570
Location:512 → 699

RhoGAP; RhoGAP: GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when ...

cl17036
Location:757 → 811

SH3; Src Homology 3 domain superfamily