Micall2 MICAL-like 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Micall2provided by MGI
Official Full Name: MICAL-like 2provided by MGI
Primary source: MGI:MGI:2444818
See related: Ensembl:ENSMUSG00000036718 AllianceGenome:MGI:2444818
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: JRAB; MICAL-L2; mFLJ00139; A930021H16Rik
Summary: Enables cytoskeletal protein binding activity and small GTPase binding activity. Involved in several processes, including actin filament polymerization; bicellular tight junction assembly; and substrate adhesion-dependent cell spreading. Acts upstream of or within endocytic recycling. Located in several cellular components, including actin filament bundle; bicellular tight junction; and recycling endosome. Colocalizes with stress fiber. Is expressed in several structures, including chondrocranium; liver lobe; medulla oblongata basal plate; submandibular gland primordium; and vibrissa. Orthologous to human MICALL2 (MICAL like 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in colon adult (RPKM 6.8), stomach adult (RPKM 6.2) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 G2; 5 78.79 cM

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (139692447..139722088, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (139706692..139736333, complement)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

the LIM domain, independent of the C-terminus, is necessary for the function of open-form JRAB/MICAL-L2
Title: Actin Cytoskeletal Reorganization Function of JRAB/MICAL-L2 Is Fine-tuned by Intramolecular Interaction between First LIM Zinc Finger and C-terminal Coiled-coil Domains.
Interaction between JRAB and filamin regulates JRABDeltaCC-induced cell spreading.
Title: Junctional Rab13-binding protein (JRAB) regulates cell spreading via filamins.
Rab13 and JRAB regulate reorganization of the actin cytoskeleton throughout epithelial junctional development.
Title: Rab13 small G protein and junctional Rab13-binding protein (JRAB) orchestrate actin cytoskeletal organization during epithelial junctional development.
show that Rab13 regulates neurite outgrowth in the rat pheochromocytoma cell line PC12 through an interaction with JRAB/MICAL-L2.
Title: Rab13 regulates neurite outgrowth in PC12 cells through its effector protein, JRAB/MICAL-L2.
These results suggest that the interaction of JRAB/MICAL-L2 with Rab8 and Rab13 coordinates the assembly of tight junctions and adherens junctions.
Title: The interaction of JRAB/MICAL-L2 with Rab8 and Rab13 coordinates the assembly of tight junctions and adherens junctions.
Although actinin-4 bound to JRAB/MICAL-L2 without Rab13, the actinin-4-JRAB/MICAL-L2 interaction was enhanced by Rab13 activation.
Title: Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions.
JRAB/MICAL-L2 mediates the endocytic recycling of occludin and the formation of functional TJs by linking Rab13 to actin cytoskeleton.
Title: JRAB/MICAL-L2 is a junctional Rab13-binding protein mediating the endocytic recycling of occludin.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D5Dcr19 (e-PCR), detects polymorphism
- UniSTS:507721

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables actin filament binding	IDA Inferred from Direct Assay more info	PubMed
enables actinin binding	IPI Inferred from Physical Interaction more info	PubMed
enables filamin binding	IDA Inferred from Direct Assay more info	PubMed
enables filamin binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables small GTPase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin filament polymerization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin filament polymerization	ISO Inferred from Sequence Orthology more info
involved_in bicellular tight junction assembly	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within endocytic recycling	IDA Inferred from Direct Assay more info	PubMed
involved_in endocytic recycling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuron projection development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuron projection development	ISO Inferred from Sequence Orthology more info
involved_in substrate adhesion-dependent cell spreading	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in actin filament bundle	IDA Inferred from Direct Assay more info	PubMed
located_in bicellular tight junction	IDA Inferred from Direct Assay more info	PubMed
located_in cell-cell junction	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in neuron projection	ISO Inferred from Sequence Orthology more info
located_in neuron projection	ISS Inferred from Sequence or Structural Similarity more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in recycling endosome	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with stress fiber	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: MICAL-like protein 2

Names: junctional Rab13-binding protein; molecule interacting with CasL-like 2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_174850.3 → NP_777275.2 MICAL-like protein 2

See identical proteins and their annotated locations for NP_777275.2

Status: VALIDATED

Source sequence(s)

AB182579, BY346995, BY700282

Consensus CDS

CCDS39353.2

UniProtKB/Swiss-Prot

Q3TN34, Q3TPY3, Q3UB65

UniProtKB/TrEMBL

Q5DU62

Related

ENSMUSP00000039707.11, ENSMUST00000044642.14

Conserved Domains (3) summary

cd09444
Location:188 → 241

LIM_Mical_like_1; This domain belongs to the LIM domain family which are found on Mical (molecule interacting with CasL) like proteins

pfam00307
Location:5 → 102

CH; Calponin homology (CH) domain

pfam12130
Location:842 → 978

DUF3585; Protein of unknown function (DUF3585)

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

139692447..139722088 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036165097.1 → XP_036020990.1 MICAL-like protein 2 isoform X1

UniProtKB/TrEMBL

E9PZD2, Q80UK4

Related

ENSMUSP00000127937.2, ENSMUST00000170773.6

Conserved Domains (4) summary

cd09444
Location:105 → 158

LIM_Mical_like_1; This domain belongs to the LIM domain family which are found on Mical (molecule interacting with CasL) like proteins

pfam05109
Location:361 → 695

Herpes_BLLF1; Herpes virus major outer envelope glycoprotein (BLLF1)

pfam12130
Location:764 → 890

DUF3585; Protein of unknown function (DUF3585)

cl00030
Location:1 → 24

CH_SF; calponin homology (CH) domain superfamily
XM_011240974.4 → XP_011239276.1 MICAL-like protein 2 isoform X1

UniProtKB/TrEMBL

E9PZD2, Q80UK4

Conserved Domains (4) summary

cd09444
Location:105 → 158

LIM_Mical_like_1; This domain belongs to the LIM domain family which are found on Mical (molecule interacting with CasL) like proteins

pfam05109
Location:361 → 695

Herpes_BLLF1; Herpes virus major outer envelope glycoprotein (BLLF1)

pfam12130
Location:764 → 890

DUF3585; Protein of unknown function (DUF3585)

cl00030
Location:1 → 24

CH_SF; calponin homology (CH) domain superfamily