Nle1 notchless homolog 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Nle1provided by MGI
Official Full Name: notchless homolog 1provided by MGI
Primary source: MGI:MGI:2429770
See related: Ensembl:ENSMUSG00000020692 AllianceGenome:MGI:2429770
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Nle; l11Jus1; l11Jus4
Summary: Acts upstream of or within several processes, including chordate embryonic development; hematopoietic stem cell homeostasis; and regulation of signal transduction. Located in nucleolus. Is expressed in several structures, including 2-cell stage embryo; gonad; hemolymphoid system gland; inner cell mass; and liver. Orthologous to human NLE1 (notchless homolog 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in ovary adult (RPKM 23.0), thymus adult (RPKM 15.4) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 C; 11 50.3 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (82791594..82799272, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (82900768..82908446, complement)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Knockdown of NLE1 inhibits development of malignant melanoma in vitro and in vivo NLE1 promotes development of malignant melanoma.
Title: Knockdown of NLE1 inhibits development of malignant melanoma in vitro and in vivo NLE1 promotes development of malignant melanoma.
activated skeletal muscle satellite cells in conditional Nle mutant mice are arrested in proliferation; however, deletion of Nle in myofibres does not impair myogenesis.
Title: Notchless defines a stage-specific requirement for ribosome biogenesis during lineage progression in adult skeletal myogenesis.
These data demonstrate an essential role for Nle1 during organogenesis and in particular during axial development.
Title: Notchless is required for axial skeleton formation in mice.
These indings establish an essential role for Nle in hematopoietic stem cells.
Title: Notchless-dependent ribosome synthesis is required for the maintenance of adult hematopoietic stem cells.
mNle is mainly required in inner cell mass cells, being instrumental for their survival, and raise the possibility that the death of mNle-deficient embryos might result from abnormal Notch signaling during the first steps of development.
Title: The murine ortholog of notchless, a direct regulator of the notch pathway in Drosophila melanogaster, is essential for survival of inner cell mass cells.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (2) 1 citation
Spontaneous (2) 1 citation
Targeted (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH125342 (e-PCR)
- UniSTS:162164

Nle1 (e-PCR), detects polymorphism
- UniSTS:259452

Nle-pending (e-PCR), detects polymorphism
- UniSTS:259453

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables molecular_function	ND No biological Data available more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within Notch signaling pathway	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within Notch signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within hematopoietic stem cell homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within inner cell mass cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within kidney development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of cysteine-type endopeptidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of canonical Wnt signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of Notch signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within ribosomal large subunit biogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within skeletal system morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within somitogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in nucleolus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleolus	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: notchless protein homolog 1

Names: Notchless gene homolog; novel WD40 repeat containing protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_145431.2 → NP_663406.2 notchless protein homolog 1

See identical proteins and their annotated locations for NP_663406.2

Status: VALIDATED

Source sequence(s)

AI852473, AK141230, BX631936, BY149184

Consensus CDS

CCDS25152.1

UniProtKB/Swiss-Prot

B1ARD5, Q8VEJ4

UniProtKB/TrEMBL

Q3URT5

Related

ENSMUSP00000099502.4, ENSMUST00000103213.10

Conserved Domains (4) summary

COG2319
Location:107 → 485

WD40; WD40 repeat [General function prediction only]

cd00200
Location:106 → 484

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

pfam08154
Location:17 → 77

NLE; NLE (NUC135) domain

sd00039
Location:159 → 197

7WD40; WD40 repeat [structural motif]

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000077.7 Reference GRCm39 C57BL/6J

Range

82791594..82799272 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030245842.1 → XP_030101702.1 notchless protein homolog 1 isoform X1

Conserved Domains (3) summary

COG2319
Location:10 → 99

WD40; WD40 repeat [General function prediction only]

cd00200
Location:58 → 394

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

sd00039
Location:69 → 107

7WD40; WD40 repeat [structural motif]