Cyth3 cytohesin 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Cyth3provided by MGI
Official Full Name: cytohesin 3provided by MGI
Primary source: MGI:MGI:1335107
See related: Ensembl:ENSMUSG00000018001 AllianceGenome:MGI:1335107
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: CLM3; Grp1; ARNO3; Pscd3
Summary: Enables phosphatidylinositol-3,4,5-trisphosphate binding activity. Acts upstream of or within establishment of epithelial cell polarity and positive regulation of cell adhesion. Located in several cellular components, including adherens junction; bicellular tight junction; and ruffle. Is expressed in brain. Orthologous to human CYTH3 (cytohesin 3). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in lung adult (RPKM 52.5), adrenal adult (RPKM 28.2) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 G2; 5 82.36 cM

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (143608202..143696005)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (143622447..143710250)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Structural organization and dynamics of Grp1 and cytohesin-2 in solution and on membranes has been described.
Title: Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes.
Structural dynamics control allosteric activation of Grp1 and ARF6.
Title: Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors.
Mutation of a key residue (K273A) within the canonical phosphatidylinositol phosphate - binding site of Grp1 significantly reduced the free energy of phosphatidylinositol phosphate binding.
Title: Association of Peripheral Membrane Proteins with Membranes: Free Energy of Binding of GRP1 PH Domain with Phosphatidylinositol Phosphate-Containing Model Bilayers.
Grp1 localizes at the beta1 integrin-positive intracellular compartment, and is involved in HGF-dependent Arf6 activation, beta1 integrin recycling and tumour angiogenesis and growth in mice.
Title: Arf6 regulates tumour angiogenesis and growth through HGF-induced endothelial β1 integrin recycling.
These observations suggest that Grp1 family guanine nucleotide exchange factors are autoregulated by mechanisms that depend on plasma membrane recruitment for activation.
Title: Structural basis and mechanism of autoregulation in 3-phosphoinositide-dependent Grp1 family Arf GTPase exchange factors.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables guanyl-nucleotide exchange factor activity	ISO Inferred from Sequence Orthology more info
enables guanyl-nucleotide exchange factor activity	ISS Inferred from Sequence or Structural Similarity more info
enables guanyl-nucleotide exchange factor activity	TAS Traceable Author Statement more info	PubMed
enables phosphatidylinositol-3,4,5-trisphosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-3,4,5-trisphosphate binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Golgi vesicle transport	ISO Inferred from Sequence Orthology more info
involved_in Golgi vesicle transport	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within establishment of epithelial cell polarity	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of cell adhesion	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of ARF protein signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in adherens junction	IDA Inferred from Direct Assay more info	PubMed
located_in bicellular tight junction	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in ruffle	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: cytohesin-3

Names: ARF nucleotide-binding site opener 3; PH, SEC7 and coiled-coil domain-containing protein 3; SEC7 homolog C; general receptor of phosphoinositides 1; mSec7-3; pleckstrin homology, Sec7 and coiled-coil domains 3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001163548.1 → NP_001157020.1 cytohesin-3 isoform 2

See identical proteins and their annotated locations for NP_001157020.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

Source sequence(s)

AI846077, AK016645, AK159229

Consensus CDS

CCDS51691.1

UniProtKB/TrEMBL

G5E8Q4, Q922J4

Related

ENSMUSP00000106355.2, ENSMUST00000110727.2

Conserved Domains (2) summary

cd01252
Location:215 → 334

PH_GRP1-like; General Receptor for Phosphoinositides-1-like Pleckstrin homology (PH) domain

pfam01369
Location:18 → 200

Sec7; Sec7 domain
NM_011182.4 → NP_035312.3 cytohesin-3 isoform 1

See identical proteins and their annotated locations for NP_035312.3

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1).

Source sequence(s)

AI846077, AK159229, BY271755

Consensus CDS

CCDS19845.1

UniProtKB/Swiss-Prot

O08967, Q8CI93

UniProtKB/TrEMBL

Q3TGW1, Q3TXK1, Q8CBE4

Related

ENSMUSP00000112157.4, ENSMUST00000116456.10

Conserved Domains (2) summary

cd01252
Location:263 → 382

PH_GRP1-like; General Receptor for Phosphoinositides-1-like Pleckstrin homology (PH) domain

pfam01369
Location:66 → 248

Sec7; Sec7 domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000071.7 Reference GRCm39 C57BL/6J

Range

143608202..143696005

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036164909.1 → XP_036020802.1 cytohesin-3 isoform X1

UniProtKB/TrEMBL

Q3TGW1, Q8CBE4

Conserved Domains (2) summary

cd01252
Location:236 → 355

PH_GRP1-like; General Receptor for Phosphoinositides-1-like Pleckstrin homology (PH) domain

pfam01369
Location:66 → 221

Sec7; Sec7 domain
XM_017320729.3 → XP_017176218.1 cytohesin-3 isoform X2

UniProtKB/TrEMBL

G5E8Q4, Q922J4

Conserved Domains (2) summary

cd01252
Location:215 → 334

PH_GRP1-like; General Receptor for Phosphoinositides-1-like Pleckstrin homology (PH) domain

pfam01369
Location:18 → 200

Sec7; Sec7 domain
XM_030254209.1 → XP_030110069.1 cytohesin-3 isoform X2

UniProtKB/TrEMBL

G5E8Q4, Q922J4

Conserved Domains (2) summary

cd01252
Location:215 → 334

PH_GRP1-like; General Receptor for Phosphoinositides-1-like Pleckstrin homology (PH) domain

pfam01369
Location:18 → 200

Sec7; Sec7 domain