Plcb2 phospholipase C, beta 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Plcb2provided by MGI
Official Full Name: phospholipase C, beta 2provided by MGI
Primary source: MGI:MGI:107465
See related: Ensembl:ENSMUSG00000040061 AllianceGenome:MGI:107465
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: B230399N12; B230205M18Rik
Summary: Predicted to enable G-protein beta/gamma-subunit complex binding activity; phosphatidylinositol phospholipase C activity; and phospholipid binding activity. Involved in phospholipase C-activating G protein-coupled receptor signaling pathway. Acts upstream of or within sensory perception of bitter taste. Located in cytoplasm. Is active in neuronal dense core vesicle. Is expressed in several structures, including early conceptus; genitourinary system; gut; immune system; and liver. Orthologous to human PLCB2 (phospholipase C beta 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in thymus adult (RPKM 18.9), spleen adult (RPKM 10.7) and 9 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 E5; 2 59.43 cM

Exon count:: 33

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (118535484..118559140, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (118705003..118728661, complement)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PLCbeta2 Promotes VEGF-Induced Vascular Permeability.
Title: PLCβ2 Promotes VEGF-Induced Vascular Permeability.
Mash1-expressing cells may be relevant to type III cells and a subset of PLCbeta2-positive cell differentiation in adult mouse taste buds.
Title: Mash1-expressing cells may be relevant to type III cells and a subset of PLCβ2-positive cell differentiation in adult mouse taste buds.
Results show that phospholipase C beta 2 (PLCbeta2) inhibits the virus-induced expression of pro-inflammatory cytokines by interacting with and inhibiting transforming growth factor-beta-activated kinase 1 (TAK1) activation.
Title: PLCβ2 negatively regulates the inflammatory response to virus infection by inhibiting phosphoinositide-mediated activation of TAK1.
Data suggest the a decrease in migration of hematopoietic stem progenitor cells (HSPCs) can be explained by impaired calcium release in phospholipase C-beta2 (PLC-beta2) knockout (KO) mice and a high baseline level of heme oxygenase 1 (HO-1), an enzyme that negatively regulates cell migration.
Title: The Involvment of Hematopoietic-Specific PLC -β2 in Homing and Engraftment of Hematopoietic Stem/Progenitor Cells.
tristetraprolin is not involved in LPS-mediated destabilization of PLCbeta-2 mRNA in macrophages
Title: 3'UTR AU-Rich Elements (AREs) and the RNA-Binding Protein Tristetraprolin (TTP) Are Not Required for the LPS-Mediated Destabilization of Phospholipase-Cβ-2 mRNA in Murine Macrophages.
Gnb isoforms control a signaling pathway comprising Rac1, Plcbeta2, and Plcbeta3 leading to LFA-1 activation and neutrophil arrest in vivo
Title: Gnb isoforms control a signaling pathway comprising Rac1, Plcβ2, and Plcβ3 leading to LFA-1 activation and neutrophil arrest in vivo.
Galphaq-coupled Lpar1 regulates cell proliferation and migration by activating two distinct PLC-beta isozymes, PLC-beta1 and PLC-beta2, respectively.
Title: Distinct phospholipase C-β isozymes mediate lysophosphatidic acid receptor 1 effects on intestinal epithelial homeostasis and wound closure.
GPCR activation of Ras and PI3Kc in neutrophils depends on PLCb2/b3 and the RasGEF RasGRP4.
Title: GPCR activation of Ras and PI3Kc in neutrophils depends on PLCb2/b3 and the RasGEF RasGRP4.
A PLCbeta/PI3Kgamma-GSK3 signaling pathway regulates cofilin phosphatase slingshot2 and neutrophil polarization and chemotaxis.
Title: A PLCβ/PI3Kγ-GSK3 signaling pathway regulates cofilin phosphatase slingshot2 and neutrophil polarization and chemotaxis.
The beta2 isoform mediates close to one-half the chemokine-induced calcium signal in resting and anti-IgM-activated B cells.
Title: Pathway-selective suppression of chemokine receptor signaling in B cells by LPS through downregulation of PLC-β2.

Submit: New GeneRIF Correction See all GeneRIFs (19)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Plcb2 (e-PCR), detects polymorphism
- UniSTS:532519

Plcb2 (e-PCR), detects polymorphism
- UniSTS:546951

AI550384 (e-PCR)
- UniSTS:185487

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables G-protein beta/gamma-subunit complex binding	IEA Inferred from Electronic Annotation more info
enables G-protein beta/gamma-subunit complex binding	ISO Inferred from Sequence Orthology more info
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables phosphatidylinositol phospholipase C activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatidylinositol phospholipase C activity	ISO Inferred from Sequence Orthology more info
enables phosphatidylinositol phospholipase C activity	ISS Inferred from Sequence or Structural Similarity more info
enables phospholipase C activity	ISO Inferred from Sequence Orthology more info
enables phospholipid binding	IEA Inferred from Electronic Annotation more info
enables phospholipid binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in G protein-coupled receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in detection of chemical stimulus involved in sensory perception of bitter taste	IEA Inferred from Electronic Annotation more info
involved_in detection of chemical stimulus involved in sensory perception of bitter taste	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	IEA Inferred from Electronic Annotation more info
involved_in lipid catabolic process	IEA Inferred from Electronic Annotation more info
involved_in phosphatidylinositol metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphatidylinositol metabolic process	ISO Inferred from Sequence Orthology more info
involved_in phosphatidylinositol metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in phosphatidylinositol-mediated signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in phospholipase C-activating G protein-coupled receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phospholipase C-activating G protein-coupled receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in release of sequestered calcium ion into cytosol	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within sensory perception of bitter taste	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of G-protein beta/gamma-subunit complex	IEA Inferred from Electronic Annotation more info
part_of G-protein beta/gamma-subunit complex	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in neuronal dense core vesicle	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2

Names: PLC-beta-2; phosphoinositide phospholipase C-beta-2

NP_001277719.1

EC 3.1.4.11

NP_808236.2

EC 3.1.4.11

XP_006498998.1

EC 3.1.4.11

XP_017171846.1

EC 3.1.4.11

XP_017171848.1

EC 3.1.4.11

XP_036015459.1

EC 3.1.4.11

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001290790.1 → NP_001277719.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform b

Status: VALIDATED

Description

Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a.

Source sequence(s)

AK031502, AK045469, AL772255, BB635904, BE991828

Consensus CDS

CCDS71117.1

UniProtKB/TrEMBL

B7ZNI1, E9PYI3

Related

ENSMUSP00000124364.2, ENSMUST00000159756.2

Conserved Domains (7) summary

cd00275
Location:659 → 780

C2_PLC_like; C2 domain present in Phosphoinositide-specific phospholipases C (PLC)

cd08624
Location:288 → 627

PI-PLCc_beta2; Catalytic domain of metazoan phosphoinositide-specific phospholipase C-beta2

PLN02222
Location:196 → 778

PLN02222; phosphoinositide phospholipase C 2

cd13361
Location:17 → 121

PH_PLC_beta; Phospholipase C-beta (PLC-beta) pleckstrin homology (PH) domain

pfam08703
Location:951 → 1126

PLC-beta_C; PLC-beta C terminal

pfam09279
Location:197 → 288

EF-hand_like; Phosphoinositide-specific phospholipase C, efhand-like

cl11531
Location:1015 → 1094

DUF904; Protein of unknown function (DUF904)
NM_177568.2 → NP_808236.2 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform a

See identical proteins and their annotated locations for NP_808236.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). The exon combination of this variant is inferred based on partial mouse transcript alignments and full-length orthologous support.

Source sequence(s)

AK045469, AK046511, AL772255, BB620972, BB635904

Consensus CDS

CCDS16582.1

UniProtKB/Swiss-Prot

A3KGF7, Q2M4J1, Q3TER8, Q8BI81

UniProtKB/TrEMBL

B7ZNI1

Related

ENSMUSP00000099583.2, ENSMUST00000102524.8

Conserved Domains (7) summary

cd00275
Location:682 → 803

C2_PLC_like; C2 domain present in Phosphoinositide-specific phospholipases C (PLC)

cd08624
Location:311 → 650

PI-PLCc_beta2; Catalytic domain of metazoan phosphoinositide-specific phospholipase C-beta2

PLN02222
Location:219 → 801

PLN02222; phosphoinositide phospholipase C 2

cd13361
Location:17 → 144

PH_PLC_beta; Phospholipase C-beta (PLC-beta) pleckstrin homology (PH) domain

pfam08703
Location:974 → 1149

PLC-beta_C; PLC-beta C terminal

pfam09279
Location:220 → 311

EF-hand_like; Phosphoinositide-specific phospholipase C, efhand-like

cl11531
Location:1038 → 1117

DUF904; Protein of unknown function (DUF904)

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000068.8 Reference GRCm39 C57BL/6J

Range

118535484..118559140 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017316357.3 → XP_017171846.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X1

UniProtKB/TrEMBL

B7ZNI1

Conserved Domains (5) summary

cd00275
Location:627 → 748

C2_PLC_like; C2 domain present in Phosphoinositide-specific phospholipases C (PLC)

cd08624
Location:256 → 595

PI-PLCc_beta2; Catalytic domain of metazoan phosphoinositide-specific phospholipase C-beta2

cd16209
Location:94 → 244

EFh_PI-PLCbeta2; EF-hand motif found in phosphoinositide phospholipase C beta 2 (PI-PLC-beta2)

pfam08703
Location:919 → 1094

PLC-beta_C; PLC-beta C terminal

pfam17787
Location:1 → 86

PH_14; PH domain
XM_017316359.3 → XP_017171848.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X2

Conserved Domains (4) summary

cd00275
Location:682 → 745

C2_PLC_like; C2 domain present in Phosphoinositide-specific phospholipases C (PLC)

cd08624
Location:311 → 650

PI-PLCc_beta2; Catalytic domain of metazoan phosphoinositide-specific phospholipase C-beta2

cd16209
Location:149 → 299

EFh_PI-PLCbeta2; EF-hand motif found in phosphoinositide phospholipase C beta 2 (PI-PLC-beta2)

pfam17787
Location:13 → 141

PH_14; PH domain
XM_036159566.1 → XP_036015459.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X3
XM_006498935.5 → XP_006498998.1 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform X4

Conserved Domains (3) summary

cd16209
Location:149 → 299

EFh_PI-PLCbeta2; EF-hand motif found in phosphoinositide phospholipase C beta 2 (PI-PLC-beta2)

pfam17787
Location:13 → 141

PH_14; PH domain

cl14615
Location:311 → 441

PI-PLCc_GDPD_SF; Catalytic domain of phosphoinositide-specific phospholipase C-like phosphodiesterases superfamily