phy-2 Prolyl 4-hydroxylase subunit alpha-2 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: phy-2
Official Full Name: Prolyl 4-hydroxylase subunit alpha-2
Primary source: WormBase:WBGene00004025
Locus tag: CELE_F35G2.4
See related: AllianceGenome:WB:WBGene00004025
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables peptidyl-proline 4-dioxygenase activity. Involved in macromolecule modification. Located in endoplasmic reticulum. Is expressed in excretory cell; hypodermis; and spermatheca. Human ortholog(s) of this gene implicated in myopia. Orthologous to several human genes including P4HA1 (prolyl 4-hydroxylase subunit alpha 1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See phy-2 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 11

Sequence:: Chromosome: IV; NC_003282.8 (12211097..12215987, complement)

Chromosome IV - NC_003282.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003282.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

although collagen P4Hs, PHY-1/PHY-2/(PDI)(2) mixed tetramer and PHY/PDI dimers, do not act on peptide substrates by a processive mechanism, prevention of hydroxylation at one catalytic site in the tetramer impairs the function of the other catalytic site
Title: Collagen prolyl 4-hydroxylase tetramers and dimers show identical decreases in Km values for peptide substrates with increasing chain length: mutation of one of the two catalytic sites in the tetramer inactivates the enzyme by more than half.
data indicate that binding sites in three PDI domains, a, b', and a', contribute to efficient C-P4H tetramer assembly
Title: Three binding sites in protein-disulfide isomerase cooperate in collagen prolyl 4-hydroxylase tetramer assembly.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

F35G2.4 (e-PCR)
- UniSTS:310140

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables L-ascorbic acid binding	IEA Inferred from Electronic Annotation more info
enables dioxygenase activity	IEA Inferred from Electronic Annotation more info
enables iron ion binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen	IEA Inferred from Electronic Annotation more info
enables peptidyl-proline 4-dioxygenase activity	IDA Inferred from Direct Assay more info	PubMed
enables procollagen-proline 4-dioxygenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables procollagen-proline 4-dioxygenase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in macromolecule modification	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum lumen	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Prolyl 4-hydroxylase subunit alpha-2

NP_502317.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003282.8 Reference assembly

Range

12211097..12215987 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_069916.7 → NP_502317.1 Prolyl 4-hydroxylase subunit alpha-2 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_502317.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q20065

Conserved Domains (3) summary

smart00702
Location:335 → 508

P4Hc; Prolyl 4-hydroxylase alpha subunit homologues

pfam08336
Location:21 → 154

P4Ha_N; Prolyl 4-Hydroxylase alpha-subunit, N-terminal region

pfam13432
Location:169 → 240

TPR_16; Tetratricopeptide repeat