lem-4 Ankyrin repeat and LEM domain-containing protein 2 homolog [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: lem-4
Official Full Name: Ankyrin repeat and LEM domain-containing protein 2 homolog
Primary source: WormBase:WBGene00021945
Locus tag: CELE_Y55F3BR.8
See related: AllianceGenome:WB:WBGene00021945
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables protein phosphatase 2A binding activity. Involved in mitotic nuclear membrane reassembly and negative regulation of phosphorylation. Located in nuclear envelope. Human ortholog(s) of this gene implicated in primary autosomal recessive microcephaly 16. Orthologous to human ANKLE2 (ankyrin repeat and LEM domain containing 2). [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See lem-4 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 6

Sequence:: Chromosome: IV; NC_003282.8 (849269..854780, complement)

Chromosome IV - NC_003282.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003282.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

By coordinating VRK-1- and PP2A-mediated signaling on BAF, Lem4 and LEM-4L (Y55F3BR.8) controls postmitotic nuclear envelope formation in a function conserved from worms to humans.
Title: Coordination of kinase and phosphatase activities by Lem4 enables nuclear envelope reassembly during mitosis.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Y55F3A_747.d (e-PCR)
- UniSTS:324432

Y55F3A_747.e (e-PCR)
- UniSTS:324433

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein phosphatase 2A binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein phosphatase 2A binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cell cycle	IEA Inferred from Electronic Annotation more info
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in mitotic nuclear membrane reassembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic nuclear membrane reassembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic nuclear membrane reassembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of phosphorylation	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in intracellular organelle	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nuclear envelope	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear membrane	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Ankyrin repeat and LEM domain-containing protein 2 homolog

NP_001023514.1

Confirmed by transcript evidence

NP_001023515.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003282.8 Reference assembly

Range

849269..854780 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001028343.3 → NP_001023514.1 Ankyrin repeat and LEM domain-containing protein 2 homolog [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001023514.1

Status: REVIEWED

UniProtKB/Swiss-Prot

H2KZB2, Q5W7E5

Conserved Domains (3) summary

cd00204
Location:144 → 263

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam13637
Location:162 → 241

Ank_4; Ankyrin repeats (many copies)

sd00045
Location:165 → 218

ANK; ANK repeat [structural motif]
NM_001028344.4 → NP_001023515.1 Ankyrin repeat and LEM domain-containing protein 2 homolog [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_001023515.1

Status: REVIEWED

UniProtKB/Swiss-Prot

H2KZB2

Conserved Domains (3) summary

cd00204
Location:121 → 240

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam13637
Location:139 → 218

Ank_4; Ankyrin repeats (many copies)

sd00045
Location:142 → 195

ANK; ANK repeat [structural motif]