dat-1 Sodium-dependent dopamine transporter [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: dat-1
Official Full Name: Sodium-dependent dopamine transporter
Primary source: WormBase:WBGene00000934
Locus tag: CELE_T23G5.5
See related: AllianceGenome:WB:WBGene00000934
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables amine transmembrane transporter activity; chloride channel activity; and dopamine:sodium symporter activity. Involved in several processes, including cellular response to amphetamine; dopamine uptake involved in synaptic transmission; and regulation of locomotion involved in locomotory behavior. Located in neuron projection membrane; neuronal cell body membrane; and presynaptic membrane. Is expressed in dopaminergic neurons; tail; and in male. Used to study dopamine beta-hydroxylase deficiency. Human ortholog(s) of this gene implicated in several diseases, including alcohol dependence; attention deficit hyperactivity disorder; classic dopamine transporter deficiency syndrome; mild cognitive impairment; and neurocirculatory asthenia. Orthologous to human SLC6A2 (solute carrier family 6 member 2) and SLC6A3 (solute carrier family 6 member 3). [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See dat-1 in Genome Data Viewer

Location:: chromosome: III

Exon count:: 13

Sequence:: Chromosome: III; NC_003281.10 (9242975..9246802)

Chromosome III - NC_003281.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003281.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Two C-terminal motifs dictate synaptic localization of DAT-1.
Title: Sequence determinants of the Caenhorhabditis elegans dopamine transporter dictating in vivo axonal export and synaptic localization.
loss of UNC-64/DAT-1 interactions leads to enhanced synaptic dopamine release
Title: Dopamine transporter/syntaxin 1A interactions regulate transporter channel activity and dopaminergic synaptic transmission.
Endogenous dopamine actions in C. elegans are tightly regulated by synaptic DAT-1.
Title: Vigorous motor activity in Caenorhabditis elegans requires efficient clearance of dopamine mediated by synaptic localization of the dopamine transporter DAT-1.
T23G5.5-transfected HeLa transports dopamine. Weaker activity for other biogenic amines (tyramine, octopamine, serotonin, norepinephrine). Inhibitors include the tricyclics imipramine and desipramine, cocaine, nomifensine, nisoxetine, and D-amphetamine.
Title: The Caenorhabditis elegans gene T23G5.5 encodes an antidepressant- and cocaine-sensitive dopamine transporter.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

T23G5.5 (e-PCR)
- UniSTS:311635

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables amine transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables chloride channel activity	IDA Inferred from Direct Assay more info	PubMed
enables dopamine:sodium symporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables dopamine:sodium symporter activity	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables symporter activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in amine transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in amino acid transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to amphetamine	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chloride transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in chloride transport	IDA Inferred from Direct Assay more info	PubMed
involved_in dopamine uptake	IDA Inferred from Direct Assay more info	PubMed
involved_in dopamine uptake involved in synaptic transmission	IBA Inferred from Biological aspect of Ancestor more info
involved_in dopamine uptake involved in synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of cellular response to manganese ion	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of cellular response to oxidopamine	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of locomotion involved in locomotory behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neurotransmitter transport	IEA Inferred from Electronic Annotation more info
involved_in norepinephrine transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in norepinephrine uptake	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of locomotion involved in locomotory behavior	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sodium ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in axolemma	IDA Inferred from Direct Assay more info	PubMed
is_active_in axon	IBA Inferred from Biological aspect of Ancestor more info
located_in dendrite membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in neuronal cell body membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in neuronal cell body membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
is_active_in presynaptic membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in presynaptic membrane	IDA Inferred from Direct Assay more info	PubMed
located_in synaptic membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Sodium-dependent dopamine transporter

NP_499043.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003281.10 Reference assembly

Range

9242975..9246802

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_066642.7 → NP_499043.1 Sodium-dependent dopamine transporter [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_499043.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q03614, Q9XTK0

Conserved Domains (1) summary

cd11556
Location:40 → 591

SLC6sbd_SERT-like_u1; uncharacterized subgroup of the SERT-like Na(+)- and Cl(-)-dependent monoamine transporter subfamily; solute binding domain