nhl-2 RING-type E3 ubiquitin transferase [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: nhl-2
Official Full Name: RING-type E3 ubiquitin transferase
Primary source: WormBase:WBGene00003598
Locus tag: CELE_F26F4.7
See related: AllianceGenome:WB:WBGene00003598
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables DEAD/H-box RNA helicase binding activity. Involved in positive regulation of development, heterochronic; protein localization to cell cortex; and regulation of establishment of cell polarity. Located in P granule and P-body. Is expressed in several structures, including body wall musculature; germ line; gonad; nervous system; and pharynx. Orthologous to human TRIM45 (tripartite motif containing 45). [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See nhl-2 in Genome Data Viewer

Location:: chromosome: III

Exon count:: 8

Sequence:: Chromosome: III; NC_003281.10 (4893658..4899085, complement)

Chromosome III - NC_003281.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003281.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Collectively, these data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma.
Title: The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. elegans.
NHL-2 is localized to cytoplasmic P-bodies and physically associates with the P-body protein CGH-1 and the core microRNA-induced silencing complex components ALG-1/2 and AIN-1.
Title: nhl-2 Modulates microRNA activity in Caenorhabditis elegans.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

F26F4.7 (e-PCR)
- UniSTS:309968

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables DEAD/H-box RNA helicase binding	IPI Inferred from Physical Interaction more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in positive regulation of development, heterochronic	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to cell cortex	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of development, heterochronic	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of establishment of cell polarity	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in P granule	IDA Inferred from Direct Assay more info	PubMed
located_in P-body	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in nucleoplasm	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: RING-type E3 ubiquitin transferase

NP_498026.1

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003281.10 Reference assembly

Range

4893658..4899085 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_065625.5 → NP_498026.1 RING-type E3 ubiquitin transferase [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_498026.1

Status: REVIEWED

UniProtKB/TrEMBL

Q19818

Conserved Domains (4) summary

smart00502
Location:280 → 406

BBC; B-Box C-terminal domain

smart00336
Location:233 → 272

BBOX; B-Box-type zinc finger

smart00184
Location:62 → 86

RING; Ring finger

cd14959
Location:729 → 1009

NHL_brat_like; NHL repeat domain of the Drosophila brain-tumor protein (brat) and similar proteins