bli-3 Dual oxidase 1 [Caenorhabditis elegans] - Gene

Summary

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Official Symbol: bli-3
Official Full Name: Dual oxidase 1
Primary source: WormBase:WBGene00000253
Locus tag: CELE_F56C11.1
See related: AllianceGenome:WB:WBGene00000253
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Caenorhabditis elegans (strain: Bristol N2)
Lineage: Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary: Enables NAD(P)H oxidase H2O2-forming activity; heme binding activity; and peroxidase activity. Involved in several processes, including cuticle development involved in collagen and cuticulin-based cuticle molting cycle; defense response to other organism; and post-embryonic body morphogenesis. Located in plasma membrane. Is expressed in hypodermis; intestine; and neurons. Used to study phenylketonuria. Human ortholog(s) of this gene implicated in congenital hypothyroidism and thyroid dyshormonogenesis 6. Orthologous to several human genes including DUOX2 (dual oxidase 2). [provided by Alliance of Genome Resources, Jan 2025]
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Genomic context

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See bli-3 in Genome Data Viewer

Location:: chromosome: I

Exon count:: 19

Sequence:: Chromosome: I; NC_003279.8 (146486..154855, complement)

Chromosome I - NC_003279.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003279.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

hpx-2 mutant animals possessed a weaker cuticle structure, indicated by higher permeability to a DNA dye, but exhibited no obvious morphological defects. In addition, fluorescent labeling of HPX-2 revealed its expression in the pharynx, an organ in which BLI-3 is also present. Interestingly, loss of HPX-2 increased intestinal colonization of E. faecalis, suggesting its role in the pharynx may limit intestinal colonization
Title: Heme peroxidase HPX-2 protects Caenorhabditis elegans from pathogens.
Here, the authors identified the Caenorhabditis elegans orthologue of mammalian mediator of ErbB2-driven cell motility, MEMO-1, as a protein that inhibits BLI-3/NADPH oxidase.
Title: NADPH oxidase-mediated redox signaling promotes oxidative stress resistance and longevity through memo-1 in C. elegans.
proline catabolism governs reactive oxygen species (ROS) homeostasis and subsequent activation of SKN-1, a critical transcription factor regulating xenobiotic stress response and pathogen defense. Intriguingly, proline catabolism-mediated activation of SKN-1 requires cell-membrane dual-oxidase Ce-Duox1/BLI-3, highlighting the importance of interaction between mitochondrial and cell-membrane components in host defense.
Title: Proline Catabolism Modulates Innate Immunity in Caenorhabditis elegans.
BLI-3/TSP-15/DOXA-1 dual oxidase complex is required for the toxic pleiotropic effects of excess iodide.
Title: The BLI-3/TSP-15/DOXA-1 dual oxidase complex is required for iodide toxicity in Caenorhabditis elegans.
We demonstrated that TSP-15, BLI-3, and DOXA-1 form complexes in vitro and in vivo. Cell-fusion-based analysis suggested that association with TSP-15 at the cell surface is crucial for BLI-3 activation to release H(2)O(2).
Title: Tetraspanin is required for generation of reactive oxygen species by the dual oxidase system in Caenorhabditis elegans.
ROS generation by Ce-Duox1/BLI-3 activates a protective SKN-1 response via p38 MAPK signaling.
Title: Ce-Duox1/BLI-3 generated reactive oxygen species trigger protective SKN-1 activity via p38 MAPK signaling during infection in C. elegans.
study of mutations of the DUOX1 truncated peroxidase domain to investigate possible role of the distal tyrosyl residue in heme binding; result argues that the DUOX peroxidase domain is primarily responsible for tyrosine cross-linking
Title: Perturbed heme binding is responsible for the blistering phenotype associated with mutations in the Caenorhabditis elegans dual oxidase 1 (DUOX1) peroxidase domain.
Extracellular dopamine potentiates kanganese-induced oxidative stress, lifespan reduction, and neurodegeneration in a BLI-3-dependent manner.
Title: Extracellular dopamine potentiates mn-induced oxidative stress, lifespan reduction, and dopaminergic neurodegeneration in a BLI-3-dependent manner in Caenorhabditis elegans.
Yeast mutants sod1Delta and yap1Delta fail to cause disease except in bli-3 worms which carry a mutation in a dual oxidase gene.
Title: A pathogenesis assay using Saccharomyces cerevisiae and Caenorhabditis elegans reveals novel roles for yeast AP-1, Yap1, and host dual oxidase BLI-3 in fungal pathogenesis.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

F56C11.1 (e-PCR)
- UniSTS:311854

F53G12.2 (e-PCR)
- UniSTS:311752

Homology

Orthologs from OrthoDB

Gene Ontology Provided by WormBase

Function	Evidence Code	Pubs
enables NAD(P)H oxidase H2O2-forming activity	IEA Inferred from Electronic Annotation more info
enables NAD(P)H oxidase H2O2-forming activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables NADH oxidase H202-forming activity	IEA Inferred from Electronic Annotation more info
enables NADPH oxidase H202-forming activity	IEA Inferred from Electronic Annotation more info
enables heme binding	IDA Inferred from Direct Assay more info	PubMed
enables heme binding	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
enables peroxidase activity	IDA Inferred from Direct Assay more info	PubMed
enables peroxidase activity	IEA Inferred from Electronic Annotation more info
enables superoxide-generating NAD(P)H oxidase activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in cellular oxidant detoxification	IEA Inferred from Electronic Annotation more info
involved_in collagen and cuticulin-based cuticle development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cuticle development involved in collagen and cuticulin-based cuticle molting cycle	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cuticle development involved in collagen and cuticulin-based cuticle molting cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in defense response	IBA Inferred from Biological aspect of Ancestor more info
involved_in defense response to Gram-positive bacterium	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in defense response to fungus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in hydrogen peroxide catabolic process	IEA Inferred from Electronic Annotation more info
involved_in nematode larval development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in post-embryonic body morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in post-embryonic body morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to oxidative stress	IEA Inferred from Electronic Annotation more info
involved_in response to oxidative stress	IGI Inferred from Genetic Interaction more info	PubMed
involved_in superoxide anion generation	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of NADPH oxidase complex	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Dual oxidase 1

NP_490686.3

Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003279.8 Reference assembly

Range

146486..154855 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_058285.5 → NP_490686.3 Dual oxidase 1 [Caenorhabditis elegans]

See identical proteins and their annotated locations for NP_490686.3

Status: REVIEWED

UniProtKB/Swiss-Prot

O61213, Q9NH90

Conserved Domains (5) summary

cd06186
Location:1218 → 1402

NOX_Duox_like_FAD_NADP; NADPH oxidase (NOX) catalyzes the generation of reactive oxygen species (ROS) such as superoxide and hydrogen peroxide. ROS were originally identified as bactericidal agents in phagocytes, but are now also implicated in cell signaling and metabolism. NOX ...

cd09820
Location:31 → 586

dual_peroxidase_like; Dual oxidase and related animal heme peroxidases

COG5126
Location:784 → 932

FRQ1; Ca2+-binding protein, EF-hand superfamily [Signal transduction mechanisms]

pfam01794
Location:1035 → 1176

Ferric_reduct; Ferric reductase like transmembrane component

pfam03098
Location:28 → 542

An_peroxidase; Animal haem peroxidase