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TBSVgp2 p33 protein [ Tomato bushy stunt virus ]

Gene ID: 1493956, updated on 6-Oct-2023

Summary

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Gene symbol
TBSVgp2
Gene description
p33 protein
Locus tag
TBSVgp2
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Tomato bushy stunt virus (strain: cherry)
Lineage
Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; Tombusviridae; Tombusvirus
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Genomic context

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Sequence:
NC_001554.1 (166..1056)

NC_001554.1Genomic Context describing neighboring genes Neighboring gene p92 protein Neighboring gene p41 capsid protein Neighboring gene p22 protein Neighboring gene p19 protein

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001554.1

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Defining the roles of cis-acting RNA elements in tombusvirus replicase assembly in vitro. Pathak KB, et al. J Virol, 2012 Jan. PMID 22013057, Free PMC Article
  2. RNA chaperone activity of the tombusviral p33 replication protein facilitates initiation of RNA synthesis by the viral RdRp in vitro. Stork J, et al. Virology, 2011 Jan 20. PMID 21071052, Free PMC Article
  3. p33-Independent activation of a truncated p92 RNA-dependent RNA polymerase of Tomato bushy stunt virus in yeast cell-free extract. Pogany J, et al. J Virol, 2012 Nov. PMID 22933278, Free PMC Article
  4. Ubiquitination of tombusvirus p33 replication protein plays a role in virus replication and binding to the host Vps23p ESCRT protein. Barajas D, et al. Virology, 2010 Feb 20. PMID 20004458, Free PMC Article
  5. Kinetics and functional studies on interaction between the replicase proteins of Tomato Bushy Stunt Virus: requirement of p33:p92 interaction for replicase assembly. Rajendran KS, et al. Virology, 2006 Feb 5. PMID 16242746

See all (13) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The authors demonstrate that tombusvirus RNA replication and the mono- and bi-ubiquitination level of p33 is decreased in rad6Delta yeast. However, plasmid-based expression of the Arabidopsis Ubc2p could complement both defects in rad6Delta yeast.
    Title: Cellular Ubc2/Rad6 E2 ubiquitin-conjugating enzyme facilitates tombusvirus replication in yeast and plants.
  2. analysis of p33-Independent activation of a truncated p92 RNA-dependent RNA polymerase of Tomato bushy stunt virus in yeast cell-free extract
    Title: p33-Independent activation of a truncated p92 RNA-dependent RNA polymerase of Tomato bushy stunt virus in yeast cell-free extract.
  3. A internal stem-loop structure and a replication silencer element are essential for viral replicase assembly.
    Title: Defining the roles of cis-acting RNA elements in tombusvirus replicase assembly in vitro.
  4. Deletion of Ino2 and Ino4 in S. cerevisiae reduces TBSV RNA accumulation and inhibits the p33 and p92 activity.
    Title: Inhibition of phospholipid biosynthesis decreases the activity of the tombusvirus replicase and alters the subcellular localization of replication proteins.
  5. The authors propose that the RNA chaperone activity of p33 facilitates the initiation of plus-strand synthesis as well as affects RNA recombination.
    Title: RNA chaperone activity of the tombusviral p33 replication protein facilitates initiation of RNA synthesis by the viral RdRp in vitro.
  6. Cpr1 and Ess1 interact with the tombusvirus p33 and inhibit viral replication in yeast model host.
    Title: Cpr1 cyclophilin and Ess1 parvulin prolyl isomerases interact with the tombusvirus replication protein and inhibit viral replication in yeast model host.
  7. In this article, the authors identify two lysines in the tombusvirus p33 replication co-factor involved in ubiquitination and show that the same lysines are also important for the p33 to interact with the host Vps23p ESCRT-I factor.
    Title: Ubiquitination of tombusvirus p33 replication protein plays a role in virus replication and binding to the host Vps23p ESCRT protein.
  8. The authors propose that ubiquitination of p33 likely alters its function or affects the recruitment of host factors during TBSV replication.
    Title: Cdc34p ubiquitin-conjugating enzyme is a component of the tombusvirus replicase complex and ubiquitinates p33 replication protein.
  9. Rpb11p affects TBSV replication and recombination only indirectly, via regulating p33 and p92(pol) levels.
    Title: Host transcription factor Rpb11p affects tombusvirus replication and recombination via regulating the accumulation of viral replication proteins.
  10. p33 interacts with p92 in vitro and that the interaction requires the S1 subdomain, whereas the S2 subdomain plays lesser function.
    Title: Kinetics and functional studies on interaction between the replicase proteins of Tomato Bushy Stunt Virus: requirement of p33:p92 interaction for replicase assembly.
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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

General protein information

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Preferred Names
p33 protein

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001554.1 Reference assembly

    Range
    166..1056
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_062898.1 p33 protein [Tomato bushy stunt virus]

    See identical proteins and their annotated locations for NP_062898.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    Q5IZF3
    Conserved Domains (1) summary
    pfam08500
    Location:145 → 290
    Tombus_P33; Tombusvirus p33
Nucleotide Protein
Heading Accession and Version

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