Actg1 actin, gamma, cytoplasmic 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Actg1provided by MGI
Official Full Name: actin, gamma, cytoplasmic 1provided by MGI
Primary source: MGI:MGI:87906
See related: Ensembl:ENSMUSG00000062825 AllianceGenome:MGI:87906
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: E51; Actg; Actl
Summary: Actins are highly conserved proteins that are involved in various types of cell motility and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta, and gamma, have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in non-muscle cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Expression: Ubiquitous expression in placenta adult (RPKM 691.0), large intestine adult (RPKM 664.6) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 11 E2; 11 84.07 cM

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	11	NC_000077.7 (120236513..120239321, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	11	NC_000077.6 (120345687..120348495, complement)

Chromosome 11 - NC_000077.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Nucleotide- and Protein-Dependent Functions of Actg1.
Title: Nucleotide- and Protein-Dependent Functions of Actg1.
Data, including data from studies using knockout mice, suggest that Actb and Actg1 are enriched in isolated membrane preparations from skeletal muscle which represent interface between mitochondria and sarco-endoplasmic reticulum; these structures appear to be important in signaling, mitochondrial dynamics, and muscle relaxation. (Actb = beta actin cytoplasmic 1; Actg1 = actin gamma cytoplasmic 1)
Title: Impaired muscle relaxation and mitochondrial fission associated with genetic ablation of cytoplasmic actin isoforms.
Two actin isoforms, beta- and gamma-actin, are crucial for slow, rapid, bulk, and overshoot endocytosis at large calyx-type synapses, and for slow endocytosis and bulk endocytosis at small hippocampal synapses.
Title: Actin Is Crucial for All Kinetically Distinguishable Forms of Endocytosis at Synapses.
Splicing to include exon 3a is concomitant with previously described down-regulation of Actg1 in differentiating C2C12 cells
Title: A novel actin mRNA splice variant regulates ACTG1 expression.
Beta- and gamma-cytoplasmic actins are required for meiosis in mouse oocytes.
Title: Beta- and gamma-cytoplasmic actins are required for meiosis in mouse oocytes.
These data support an essential role for beta-actin in regulating cell migration and gene expression through control of the cellular G-actin pool
Title: β-Actin specifically controls cell growth, migration, and the G-actin pool.
Results demonstrate overlapping developmental roles but unique post-developmental functions for beta-actin and gamma-actin in maintaining hair cell stereocilia.
Title: β-actin and γ-actin are each dispensable for auditory hair cell development but required for Stereocilia maintenance.
Since the total amount of actin protein was maintained in Actg1(-/-) cells, our data suggests a distinct requirement for gamma(cyto)-actin in cell growth and survival.
Title: Delayed embryonic development and impaired cell growth and survival in Actg1 null mice.
arginylated gamma-actin, unlike beta-, was highly unstable and selectively ubiquitinated and degraded in vivo; instability was regulated by differences in nucleotide coding sequence between the actin isoforms, which conferred different translation rates
Title: Differential arginylation of actin isoforms is regulated by coding sequence-dependent degradation.
Eplin-alpha transcription is regulated by actin-MAL-SRF signalling.
Title: Epithelial Protein Lost in Neoplasm alpha (Eplin-alpha) is transcriptionally regulated by G-actin and MAL/MRTF coactivators.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

PMC156124P2 (e-PCR)
- UniSTS:271360

PMC137792P1 (e-PCR)
- UniSTS:270855

RH125494 (e-PCR)
- UniSTS:162312

PMC201106P1 (e-PCR)
- UniSTS:271883

PMC33464P1 (e-PCR)
- UniSTS:273163

PMC138261P1 (e-PCR)
- UniSTS:270864

PMC156330P1 (e-PCR)
- UniSTS:271392

RH128697 (e-PCR)
- UniSTS:212005

PMC108612P1 (e-PCR)
- UniSTS:270141

PMC23952P1 (e-PCR)
- UniSTS:272216

PMC109689P1 (e-PCR)
- UniSTS:270178

PMC302066P1 (e-PCR)
- UniSTS:272483

PMC110143P1 (e-PCR)
- UniSTS:270184

PMC110200P1 (e-PCR)
- UniSTS:270185

PMC24561P1 (e-PCR)
- UniSTS:272264

PMC305873P2 (e-PCR)
- UniSTS:272568

Acta1 (e-PCR), detects polymorphism
- UniSTS:141013

RH125445 (e-PCR)
- UniSTS:162264

PMC97568P1 (e-PCR)
- UniSTS:273653

PMC22644P1 (e-PCR)
- UniSTS:272120

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables profilin binding	IEA Inferred from Electronic Annotation more info
enables profilin binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase binding	IBA Inferred from Biological aspect of Ancestor more info
enables structural constituent of cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
enables structural constituent of postsynaptic actin cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
enables structural constituent of postsynaptic actin cytoskeleton	ISO Inferred from Sequence Orthology more info
enables ubiquitin protein ligase binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in angiogenesis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within_positive_effect angiogenesis	ISO Inferred from Sequence Orthology more info
involved_in axonogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell motility	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within cellular response to type II interferon	IDA Inferred from Direct Assay more info	PubMed
involved_in morphogenesis of a polarized epithelium	IEA Inferred from Electronic Annotation more info
involved_in morphogenesis of a polarized epithelium	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell migration	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of gene expression	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of wound healing	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of wound healing	ISO Inferred from Sequence Orthology more info
involved_in protein localization to bicellular tight junction	IEA Inferred from Electronic Annotation more info
involved_in protein localization to bicellular tight junction	ISO Inferred from Sequence Orthology more info
involved_in regulation of focal adhesion assembly	IEA Inferred from Electronic Annotation more info
involved_in regulation of focal adhesion assembly	ISO Inferred from Sequence Orthology more info
involved_in regulation of stress fiber assembly	IEA Inferred from Electronic Annotation more info
involved_in regulation of stress fiber assembly	ISO Inferred from Sequence Orthology more info
involved_in regulation of synaptic vesicle endocytosis	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of synaptic vesicle endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transepithelial transport	IEA Inferred from Electronic Annotation more info
involved_in regulation of transepithelial transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within sarcomere organization	IDA Inferred from Direct Assay more info	PubMed
involved_in tight junction assembly	IEA Inferred from Electronic Annotation more info
involved_in tight junction assembly	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of NuA4 histone acetyltransferase complex	IBA Inferred from Biological aspect of Ancestor more info
is_active_in Schaffer collateral - CA1 synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in Schaffer collateral - CA1 synapse	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in actin cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in actin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
is_active_in actin filament	IBA Inferred from Biological aspect of Ancestor more info
located_in actin filament	ISO Inferred from Sequence Orthology more info
located_in apical junction complex	IEA Inferred from Electronic Annotation more info
located_in apical junction complex	ISO Inferred from Sequence Orthology more info
located_in apical part of cell	IDA Inferred from Direct Assay more info	PubMed
is_active_in axon	IBA Inferred from Biological aspect of Ancestor more info
located_in basal body patch	IDA Inferred from Direct Assay more info	PubMed
is_active_in calyx of Held	IDA Inferred from Direct Assay more info	PubMed
is_active_in calyx of Held	IMP Inferred from Mutant Phenotype more info	PubMed
located_in cell-cell junction	ISO Inferred from Sequence Orthology more info
located_in costamere	TAS Traceable Author Statement more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	TAS Traceable Author Statement more info
located_in dense body	ISS Inferred from Sequence or Structural Similarity more info
located_in extracellular exosome	IEA Inferred from Electronic Annotation more info
located_in extracellular exosome	ISO Inferred from Sequence Orthology more info
part_of filamentous actin	IDA Inferred from Direct Assay more info	PubMed
located_in focal adhesion	ISS Inferred from Sequence or Structural Similarity more info
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in myelin sheath	HDA more info	PubMed
located_in myofibril	IDA Inferred from Direct Assay more info	PubMed
located_in phagocytic vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
is_active_in postsynaptic actin cytoskeleton	ISO Inferred from Sequence Orthology more info
is_active_in presynaptic actin cytoskeleton	ISO Inferred from Sequence Orthology more info
is_active_in synapse	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: actin, cytoplasmic 2

Names: beta-actin FE-3; gamma-actin

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001313923.1 → NP_001300852.1 actin, cytoplasmic 2 isoform 2

Status: REVIEWED

Description

Transcript Variant: This variant (2) contains an alternate exon, which causes translation initiation at a downstream AUG compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1.

Source sequence(s)

AK153446, BU706992, CX567990

UniProtKB/TrEMBL

E9Q1F2

Conserved Domains (1) summary

cl17037
Location:1 → 253

NBD_sugar-kinase_HSP70_actin; Nucleotide-Binding Domain of the sugar kinase/HSP70/actin superfamily
NM_009609.3 → NP_033739.1 actin, cytoplasmic 2 isoform 1

See identical proteins and their annotated locations for NP_033739.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1).

Source sequence(s)

AK153446, CX567990

Consensus CDS

CCDS25730.1

UniProtKB/Swiss-Prot

P63260

UniProtKB/TrEMBL

Q3TSB7, Q4KL81

Related

ENSMUSP00000071486.7, ENSMUST00000071555.13

Conserved Domains (1) summary

PTZ00281
Location:1 → 375

PTZ00281; actin; Provisional