GEO DataSets

(Submitter supplied) We explored the role of triglyceride (TAG) mobilization in the reproductive success of female Anopheles gambiae after a blood meal. Silencing triglyceride lipase 2 (TL2) in An. gambiae reduced egg production and abrogates fertility. Embryos from AgTL2-depleted females develop normally but fail to hatch and are characterized by reduced levels of lipids and key metabolites, including carbohydrates, Tricarboxylic acid (TCA) intermediates, paralleled by increased levels of purine and pyrimidine breakdown products, signifying possible cellular death. more...

Organism:

Anopheles gambiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33392

24 Samples

Download data: TXT

(Submitter supplied) Alternative splicing (AS) is a highly conserved mechanism that allows to expand the coding capacity of the genome, by modifying how multiple isoforms are expressed or used to generate different phenotypes. Despite its importance in physiology and disease, genome-wide studies of AS are lacking in most insects, including mosquitoes. Even for model organisms, chromatin associated processes involved in the regulation AS are poorly known. more...

Organism:

Anopheles gambiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17644

4 Samples

Download data: TXT

(Submitter supplied) Gene edited mosquitoes lacking a gamma-interferon-inducible lysosomal thiol reductase-like protein, namely (mosGILTnull) have decreased Plasmodium infection, which is linked to impaired ovarian development and immune activation. The transcriptome of A. gambiae mosGILTnull was therefore compared to wild type (WT) by RNA-sequencing to delineate mosGILT-dependent pathways. Compared to WT mosquitoes, mosGILTnull A. more...

Organism:

Anopheles gambiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32063

16 Samples

Download data: TXT

(Submitter supplied) Malaria control relies on insecticides targeting the mosquito vector, but is being increasingly compromised by insecticide resistance. Elevated expression of metabolic enzymes frequently drives resistance. In diploids, gene expression is regulated both in cis, by regulatory sequences on the same chromosome, and by trans acting factors, affecting both alleles equally. Differing levels of transcription can be caused by mutations in cis-regulatory modules, but few cis-regulatory modules controlling the expression of genes that determine insecticide resistance have been identified. more...

Organism:

Anopheles gambiae

Type:

Genome variation profiling by high throughput sequencing

Platform:

GPL25377

6 Samples

Download data: TXT

(Submitter supplied) In this study we determine the effects of silencing adiponectin receptor in A. gambiae mosquitoes after taking Plasmodium gambiae infected blood meals. dsRNA (dsAdpR and dsGluc) microinjected A. gambiae mosquitoes were fed on P. berghei-infected Swiss Webster mice. Two days after a blood meal, the mosquitoes were collected for midgut dissection.

Organism:

Anopheles gambiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32063

8 Samples

Download data: TXT

(Submitter supplied) The Anopheles mosquito is one of thousands of species in which sex differences play a central role in their biology, as only females need a blood meal in order to produce eggs. Sex differentiation is regulated by sex chromosomes, but their presence creates a dosage imbalance between males (XY) and females (XX). Dosage compensation (DC) can re-equilibrate the expression of sex-chromosomal genes, but because DC mechanisms have only been fully characterized in a few model organisms, key questions about its evolutionary diversity and functional necessity remain unresolved. more...

Organism:

Anopheles gambiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22033

7 Samples

Download data: BW, TSV

(Submitter supplied) The Anopheles mosquito is one of thousands of species in which sex differences play a central role in their biology, as only females need a blood meal in order to produce eggs. Sex differentiation is regulated by sex chromosomes, but their presence creates a dosage imbalance between males (XY) and females (XX). Dosage compensation (DC) can re-equilibrate the expression of sex-chromosomal genes, but because DC mechanisms have only been fully characterized in a few model organisms, key questions about its evolutionary diversity and functional necessity remain unresolved. more...

Organism:

Anopheles gambiae

Type:

Other

Platform:

GPL22033

7 Samples

Download data: BW

(Submitter supplied) The Anopheles mosquito is one of thousands of species in which sex differences play a central role in their biology, as only females need a blood meal in order to produce eggs. Sex differentiation is regulated by sex chromosomes, but their presence creates a dosage imbalance between males (XY) and females (XX). Dosage compensation (DC) can re-equilibrate the expression of sex-chromosomal genes, but because DC mechanisms have only been fully characterized in a few model organisms, key questions about its evolutionary diversity and functional necessity remain unresolved. more...

Organism:

Anopheles gambiae

Type:

Other

Platform:

GPL22033

8 Samples

Download data: BW

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Anopheles gambiae

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL22033

79 Samples

Download data: BW

(Submitter supplied) Differences between males and females are central to the biology of thousands of species across the tree of life. Sex chromosomes play a key role, but despite their their evolutionary diversity, the regulatory mechanisms have been mostly elucidated in the three model species Mammals, D. melanogaster and C. elegans. Here we present the characterization of the first X chromosome dosage compensation (DC) pathway in a non-model organism, the malaria mosquito Anopheles gambiae. more...

Organism:

Anopheles gambiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22033

8 Samples

Download data: BW, TSV

(Submitter supplied) Differences between males and females are central to the biology of thousands of species across the tree of life. Sex chromosomes play a key role, but despite their their evolutionary diversity, the regulatory mechanisms have been mostly elucidated in the three model species Mammals, D. melanogaster and C. elegans. Here we present the characterization of the first X chromosome dosage compensation (DC) pathway in a non-model organism, the malaria mosquito Anopheles gambiae. more...

Organism:

Anopheles gambiae

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL22033

20 Samples

Download data: BW, XLSX

(Submitter supplied) Differences between males and females are central to the biology of thousands of species across the tree of life. Sex chromosomes play a key role, but despite their their evolutionary diversity, the regulatory mechanisms have been mostly elucidated in the three model species Mammals, D. melanogaster and C. elegans. Here we present the characterization of the first X chromosome dosage compensation (DC) pathway in a non-model organism, the malaria mosquito Anopheles gambiae. more...

Organism:

Anopheles gambiae

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL22033

8 Samples

Download data: BW

(Submitter supplied) Differences between males and females are central to the biology of thousands of species across the tree of life. Sex chromosomes play a key role, but despite their their evolutionary diversity, the regulatory mechanisms have been mostly elucidated in the three model species Mammals, D. melanogaster and C. elegans. Here we present the characterization of the first X chromosome dosage compensation (DC) pathway in a non-model organism, the malaria mosquito Anopheles gambiae. more...

Organism:

Anopheles gambiae

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL22033

12 Samples

Download data: BW

(Submitter supplied) Differences between males and females are central to the biology of thousands of species across the tree of life. Sex chromosomes play a key role, but despite their their evolutionary diversity, the regulatory mechanisms have been mostly elucidated in the three model species Mammals, D. melanogaster and C. elegans. Here we present the characterization of the first X chromosome dosage compensation (DC) pathway in a non-model organism, the malaria mosquito Anopheles gambiae. more...

Organism:

Anopheles gambiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22033

9 Samples

Download data: BW, TSV

(Submitter supplied) In many species, males and females display differences in their physiology that are not limited to the gonads. Sex differences in adult insects can comprise body size and feeding behaviour, as only female, but not male mosquitoes are blood-feeding in order to produce eggs. This is relevant since the malaria-causing parasite Plasmodium is transmitted by a female mosquito through a previous blood meal from an infected individual. more...

Organism:

Anopheles gambiae

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL28724 GPL22033

69 Samples

Download data: BW, TSV

(Submitter supplied) Malaria mosquitoes acoustically detect their mating partners within large swarms that form transiently at dusk. Indeed, male malaria mosquitoes preferably respond to female flight tones during swarm time. This phenomenon implies a sophisticated context- and time-dependent modulation of mosquito audition, the mechanisms of which are largely unknown. Using transcriptomics, we identify a complex network of candidate neuromodulators regulating mosquito hearing in the species Anopheles gambiae. more...

Organism:

Anopheles gambiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22033

36 Samples

Download data: TSV

(Submitter supplied) We custom-built a bioinformatics pipeline to search for 20E-modifying enzymes in the accessory glands of Anopheles gambiae males, searching for ecdysteroid kinases (EcK), ecdysone oxidases (EO), and ecdysteroid-phosphate phosphatases (EPP). To this end, we generated RNAseq datasets of different An. gambiae tissues dissected from virgin and mated females and males, and produced similar datasets for Anopheles albimanus, a South American species that does not synthetize and transfer ecdysteroids during mating. more...

Organism:

Anopheles albimanus; Anopheles gambiae

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL32062 GPL32063

88 Samples

Download data: TXT

(Submitter supplied) The heavy use of pesticides in intensive agricultural areas often leads to the contamination of neighbouring mosquito larvae breeding sites. Such exposure to complex agrochemical mixtures can affect the tolerance of mosquitoes larvae to insecticides. The objective of this study was to determine whether agrochemical pollutants found in Anopheline larval breeding sites can affect the tolerance of adults to Fludora® Fusion, a novel vector control insecticide formulation combining two insecticides with distinct modes of action. more...

Organism:

Anopheles gambiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22033

16 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Anopheles gambiae; Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL25232 GPL23323

32 Samples

Download data: BW

(Submitter supplied) Heteromorphic sex chromosomes induce potentially deleterious gene expression imbalances that are frequently corrected by dosage compensation (DC). Three distinct molecular strategies to achieve DC have been previously described in nematodes, fruit flies and mammals. The reason for these mechanistic differences remain unclear: Are they a consequence of distinct genomes and gene content, functional or ecological constraints, or random initial commitment to an evolutionary trajectory? Here, we study DC in the malaria mosquito Anopheles gambiae. more...

Organism:

Anopheles gambiae

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL25232

12 Samples

Download data: BW