GEO DataSets

(Submitter supplied) Differential RNA-Seq data for Vibrio campbellii BB120 (ATCC BAA-1116, previously designated as Vibrio harveyi)

Organism:

Vibrio campbellii

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27117

12 Samples

Download data: WIG

(Submitter supplied) Vibrio campbellii is a gram-negative bacterial pathogen that is both free-living and a pathogen of marine organisms and exhibits swimming motility via a single, polar flagellum. Swimming motility is a critical virulence factor in V. campbellii pathogenesis, and disruption of the flagellar motor significantly decreases host mortality. However, while V. campbelli encodes homologs of flagellar and chemotaxis genes conserved by other members of the Vibrionaceae, the regulatory network governing these genes have not been explored. more...

Organism:

Vibrio campbellii

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27117

15 Samples

Download data: TXT

(Submitter supplied) Vibrio campbellii BB120 (ATCC BAA-1116, previously designated as Vibrio harveyi) is a fundamental model strain for studying population density-based cell-to-cell communication, known as quorum sensing, among gram-negative bacteria. In V. campbellii BB120, sensing of autoinducers at high cell densities activates the expression of the master transcriptional regulator, LuxR, which controls the expression of genes involved in group behaviors. more...

Organism:

Vibrio campbellii

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27117

12 Samples

Download data: TXT

(Submitter supplied) Quorum sensing controls hundreds of genes in vibrios required for cell density-specific behaviors, including bioluminescence, biofilm formation, competence, secretion, and motility. The central regulator in the quorum sensing pathway in vibrios is LuxR/HapR, which directly regulates >100 genes in the 625-gene regulon of Vibrio harveyi. Among these directly regulated genes are 15 transcription factors, which we predicted would comprise the second tier in the hierarchy of the quorum sensing regulon. more...

Organism:

Vibrio campbellii

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27117

30 Samples

Download data: TXT

(Submitter supplied) In marine Vibrio species, chitin-induced natural transformation enables bacteria to take up DNA from the external environment and integrate it into their genome via homologous recombination. Expression of the master competence regulator TfoX bypasses the need for chitin induction and drives expression of the genes required for competence in several Vibrio species. Here, we show that TfoX expression in two Vibrio campbellii strains, DS40M4 and NBRC 15631, enables high frequencies of natural transformation. more...

Organism:

Vibrio campbellii

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27117

12 Samples

Download data: TXT

(Submitter supplied) Bacteria coordinate cellular behaviors using a cell-cell communication system termed quorum sensing. In Vibrio harveyi, the master quorum sensing transcriptional factor LuxR directly regulates >100 genes in response to changes in population density. Here, we show that LuxR derepresses quorum sensing loci by competing with H-NS, a global transcriptional repressor that oligomerizes on DNA to form filaments and bridges. more...

Organism:

Vibrio campbellii

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27117

12 Samples

Download data: TXT

(Submitter supplied) Bacteria coordinate cellular behaviors using a cell-cell communication system termed quorum sensing. In Vibrio harveyi, the master quorum sensing transcriptional factor LuxR directly regulates >100 genes in response to changes in population density. Here, we show that LuxR derepresses quorum sensing loci by competing with H-NS, a global transcriptional repressor that oligomerizes on DNA to form filaments and bridges. more...

Organism:

Vibrio campbellii

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL27117

5 Samples

Download data: TXT

(Submitter supplied) Vibrio campbellii BAA-1116 was used as a Harveyi clade model organism to determine the impact of indole signaling on virulence. Gene expression analysis of V. campbellii grown in LB35 broth with or without 100 μM indole revealed that indole decreased: (1) V. campbellii virulence in shrimp and prawn challenge assays, (2) exopolysaccharide production, and (3) swimming motility. The results also indicated that indole inhibits quorum sensing-regulated bioluminescence and blocks the three-channel quorum sensing system by interfering with quorum sensing signal transduction.

Organism:

Vibrio campbellii CAIM 519 = NBRC 15631 = ATCC 25920; Vibrio campbellii

Type:

Expression profiling by array

Platform:

GPL10230

10 Samples

Download data: CEL

(Submitter supplied) Vibrio campbellii BAA-1116 was used as model organism from the Harveyi clade to understand how melanization affected cellular phenotype, metabolism and virulence. An in-frame deletion of the homogentisate-1,2-dioxygenase (hmgA) gene resulted in the overproduction of a pigment in cell culture supernatants and cellular membranes that was identified as pyomelanin. Unlike previous demonstrations in Vibrio cholerae, Burkholderia cepacia and Pseudomonas aeruginosa, the pigmented V. more...

Organism:

Vibrio campbellii CAIM 519 = NBRC 15631 = ATCC 25920; Vibrio campbellii

Type:

Expression profiling by array

Platform:

GPL10230

6 Samples

Download data: CEL

(Submitter supplied) Although many members of the genus Vibrio are known to inhabit the marine photic zone, an understanding of the influence of light on the molecular physiology of Vibrio spp. has largely been neglected. To begin to characterize the photophysiology of one such Vibrio sp. (Vibrio campbellii ATCC strain BAA-1116) we used microarray-based expression profiling to compare the transcriptomes of illuminated versus dark cell cultures. more...

Organism:

Vibrio campbellii CAIM 519 = NBRC 15631 = ATCC 25920

Type:

Expression profiling by array

Platform:

GPL10230

10 Samples

Download data: CEL

(Submitter supplied) The selective advantage of bioluminescence in bacterial cells that do not form symbiotic relationships with aquatic animals is still not known. Some evidence suggests that bioluminescence plays a role in DNA repair by a photoreactivation process (Czyz 2000) and that non-bioluminescent strains are less virulent than their bioluminescent isogenic counterparts (Ruwandeepika 2010). All hypotheses to date suggest bioluminescence associated or mediated changes in gene expression, yet the evidence for this does not exist. more...

Organism:

Vibrio campbellii CAIM 519 = NBRC 15631 = ATCC 25920; Vibrio campbellii

Type:

Expression profiling by array

Platform:

GPL10230

6 Samples

Download data: CEL

(Submitter supplied) Small RNA and regulatory RNA discovery has been based on (i) computational predictions mainly in intergenic regions, (ii) microarray experiments, (iii) shotgun cloning of cDNA libraries, (iv) classical cloning of abundant small RNAs after size fractionation in polyacrylamide gels and (v) copurification with proteins like Hfq, CsrA and RNA polymerase. In this study, we attempted to experimentally validate in silico small and regulatory RNA predictions for V. more...

Organism:

Vibrio campbellii CAIM 519 = NBRC 15631 = ATCC 25920

Type:

Expression profiling by array

Platform:

GPL10230

5 Samples

Download data: CEL

(Submitter supplied) Affymetrix DNA microarray (Vharveyi520694F) that targets 4831 total CDS from the fully assembled and annotated V. harveyi BAA-1116 genome (Naval Research Laboratory sequencing effort GenBank CP001223-5) Protocol: see manufacturer's web site

Organism:

Vibrio campbellii CAIM 519 = NBRC 15631 = ATCC 25920

5 Series

37 Samples

Download data: CDF, TXT