GEO DataSets

(Submitter supplied) Pectobacterium carotovorum ssp. carotovorum (Pcc) is a necrotrophic bacterial species that causes soft rot disease in Chinese cabbage. In this study, plants harboring the resistant mutant sr gene, which confers resistance against Pcc,were screened from an 800 M2 population mutated by ethyl methane sulfonate (EMS) and scored in vitro and in vivo for lesion size. The transcript profiles showed ~512 differentially expressed genes (DEGs) between sr and WT plants occurring between 6 and 12 h postinoculation (hpi), which corresponded to the important defense regulation period (resistance) to Pcc in Chinese cabbage. more...

Organism:

Brassica rapa subsp. pekinensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24709

24 Samples

Download data: TXT

(Submitter supplied) Anther development is a complex process, and the study of its molecular mechanism has an important impact on plant breeding. This study aims to identify microRNA (miRNA), mRNA, long non-coding RNA (lncRNA), and circular RNA (circRNA) related to anther development of Chinese cabbage, so as to construct competitive endogenous RNA (ceRNA) regulatory networks and provide valuable knowledge for the exploration of pollen development mechanism of Chinese cabbage. more...

Organism:

Brassica rapa subsp. pekinensis

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL24709

2 Samples

Download data: TXT

(Submitter supplied) Anther development is a complex process, and the study of its molecular mechanism has an important impact on plant breeding. This study aims to identify microRNA (miRNA), mRNA, long non-coding RNA (lncRNA), and circular RNA (circRNA) related to anther development of Chinese cabbage, so as to construct competitive endogenous RNA (ceRNA) regulatory networks and provide valuable knowledge for the exploration of pollen development mechanism of Chinese cabbage. more...

Organism:

Brassica rapa subsp. pekinensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29989

2 Samples

Download data: TXT

(Submitter supplied) The transition from vegetative growth to reproductive growth involves many pathways. Vernalization is crucial to the formation of floral organs, the regulation of flowering time and plant breeding. The purpose of this study was to identify the mRNA, microRNA (miRNA), long non-coding RNA (lncRNA), and circular RNA (circRNA) related to vernalization of Chinese cabbage, and to construct a competitive endogenous RNA (ceRNA) network, so as to provide valuable information for exploring the molecular mechanism of vernalization of Chinese cabbage. more...

Organism:

Brassica rapa subsp. pekinensis

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL24709

6 Samples

Download data: TXT

(Submitter supplied) The transition from vegetative growth to reproductive growth involves many pathways. Vernalization is crucial to the formation of floral organs, the regulation of flowering time and plant breeding. The purpose of this study was to identify the mRNA, microRNA (miRNA), long non-coding RNA (lncRNA), and circular RNA (circRNA) related to vernalization of Chinese cabbage, and to construct a competitive endogenous RNA (ceRNA) network, so as to provide valuable information for exploring the molecular mechanism of vernalization of Chinese cabbage. more...

Organism:

Brassica rapa subsp. pekinensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29989

6 Samples

Download data: TXT

(Submitter supplied) To better examine the molecular mechanisms behind the virus infection, we conducted a correlation analysis of RNA-Seq and quantitative iTRAQ-LC-MS/MS in TuMV-infected and in healthy Chinese cabbage leaves.

Organism:

Brassica rapa subsp. pekinensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17788

6 Samples

Download data: TXT

(Submitter supplied) We analyze the differentially expressed genes (DEGs) in RNA-seq data.

Organism:

Brassica rapa subsp. pekinensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17788

8 Samples

Download data: XLSX

(Submitter supplied) The hybrid seed production was performed using the male sterility line, which is an important way of heterosis utilization in Chinese cabbage. A stably inherited male sterile mutant msm was obtained from a Chinese cabbage DH line ‘FT’ using the isolated microspore culture combined with 60Co γ-rays mutagenesis. Compared to the wild type ‘FT’, the msm exhibited completely degenerated stamens and no pollen phenotype, and other characters had no significant difference except for stamen. more...

Organism:

Brassica rapa subsp. pekinensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17788

6 Samples

Download data: TXT

(Submitter supplied) Methionine sulfoxide reductases catalyze the reduction of MetSO back to the correct Met residue. Previously, the gene of Capsicum annuum methionine sulfoxide reductase B2 was isolated and CaMSRB2-overexpressing tomato shows enhanced growth, which may trigger increased resistance to the pathogens. To assess the role of this enzyme in rice, we generated transgenic lines under the control of the rice Rab21 (responsive to ABA protein 21) promoter with/without Bar marker gene. more...

Organism:

Brassica rapa; Brassica rapa subsp. pekinensis

Type:

Expression profiling by array

Platform:

GPL24931

6 Samples

Download data: PAIR, PROBE

(Submitter supplied) We conducted genome-wide transcriptome analysis using the inbred Chinese cabbage line, ‘4004’, which displayed early flowering in response to vernalization. A total of 1,677 differentially-expressed genes (DEGs) were identified with and without vernalization. Transcriptome analysis identified 223 homologs of Arabidopsis Ft genes in Chinese cabbage, and 50 of these genes responded to vernalization. RT-qPCR analysis of major Ft genes showed that the majority of flowering enhancers were up-regulated in response to vernalization, whereas most flowering repressors were down-regulated in response to vernalization. more...

Organism:

Brassica rapa subsp. pekinensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17788

4 Samples

Download data: TXT

(Submitter supplied) We performed microRNA profiling of doubled haploids and autotetraploids that generated from a Chinese cabbage doubled haploid line ‘FT’ using the Illumina high-throughput sequencing platform and analyzed differentially expressed miRNAs.

Organism:

Brassica rapa subsp. pekinensis

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL24709

2 Samples

Download data: FA, XLS

(Submitter supplied) We performed transcript profiling of doubled haploids and autotetraploids that generated from a Chinese cabbage doubled haploid line ‘FT’ using the Illumina high-throughput sequencing platform and analyzed differential gene expression at the transcriptional level.

Organism:

Brassica rapa subsp. pekinensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24708

6 Samples

Download data: FASTA, XLS

(Submitter supplied) We performed microRNA profiling of doubled haploids and autotetraploids that generated from a Chinese cabbage doubled haploid line ‘FT’ using the Illumina high-throughput sequencing platform and analyzed differentially expressed miRNAs.

Organism:

Brassica rapa subsp. pekinensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24709

2 Samples

Download data: FA, TXT

(Submitter supplied) We performed transcript profiling of doubled haploids and autotetraploids that generated from a Chinese cabbage doubled haploid line ‘FT’ using the Illumina high-throughput sequencing platform and analyzed differential gene expression at the transcriptional level.

Organism:

Brassica rapa subsp. pekinensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24708

6 Samples

Download data: FASTA, TXT

(Submitter supplied) We studied the causes of sterility underlying multiple-allele-inherited male sterility in Chinese cabbage by identifying differentially expressed genes (DEGs) related to pollen sterility between fertile and sterile flower buds. In this work,we performed transcriptome analysis of mRNA isolated from fertile and sterile buds using Illumina HiSeq 2000 platform sequencing. Approximately 80% of ~229 million high quality paired-end reads were uniquely mapped to the reference genome. more...

Organism:

Brassica rapa subsp. pekinensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17788

6 Samples

Download data: XLS

(Submitter supplied) Using 300K′-high density microarray covering the chinese cabbage whole genome, genome-wide expression analyses of cold stress conditions.

Organism:

Brassica rapa subsp. pekinensis; Brassica rapa

Type:

Expression profiling by array

Platform:

GPL17248

10 Samples

Download data: CALLS, PAIR, PROBE

(Submitter supplied) The female sterility mutants are ideal materials for studying the pistil development in plants. We identified a female sterility mutant (fsm) exhibiting stable inheritance, which was derived from a Chinese cabbage DH line ‘FT’ using the combination of isolated microspore culture and ethyl methanesulfonate (EMS) mutagenesis. Genetic analysis indicated that the phenotype of fsm was controlled by a single recessive nuclear gene. more...

Organism:

Brassica rapa subsp. pekinensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17788

6 Samples

Download data: TXT

(Submitter supplied) we performed transcript profiling of male sterile and fertile buds from a multiple-allele inherited male sterile AB line using the Illumina high-throughput sequencing platform and analyzed differential gene expression at the transcriptional level.

Organism:

Brassica rapa subsp. pekinensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17788

2 Samples

Download data: TXT, XLS, XLSX

(Submitter supplied) We conducted a RNA-Seq analysis of MeJA-treated Chinese cabbage leaf transcriptome. Total 14,619,469 sequence reads were generated to produce 27,461 detected genes, among which 1,451 genes were up-regulated and 991 genes were down-regulated as differentially expressed genes (DEGs) (log2 ratio ≥1, false discovery rate ≤0.001). More than 90% of the DEGs (2,278) were between 1.0- and 3.0-fold (log2 ratio). more...

Organism:

Brassica rapa subsp. pekinensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17788

2 Samples

Download data: TXT

(Submitter supplied) To identify genes associated with genic male sterility (GMS) that could be useful for hybrid breeding in Chinese cabbage (Brassica rapa ssp. pekinensis), floral bud transcriptome analysis was carried out using a B. rapa microarray with 300,000 probes (Br300K). Among 47,548 clones deposited on a Br300K microarray with seven probes of 60 nt length within the 3' 150 bp region, a total of 10,622 genes were differentially expressed between fertile and sterile floral buds; 4,774 and 5,848 genes were up-regulated over 2-fold in fertile and sterile buds, respectively. more...

Organism:

Brassica rapa; Brassica rapa subsp. pekinensis

Type:

Expression profiling by array

Platform:

GPL17248

14 Samples

Download data: PAIR, PROBE