GEO DataSets

(Submitter supplied) We studied the application of transcriptome technology in alfalfa selenium treatment. After spraying sodium selenite on the leaves, the process of selenium absorption and assimilation of alfalfa is unknown. The time point of transcriptome determination was determined by measuring the change of selenium content. Our results showed that 12 h was the key point of the change of selenium content in alfalfa, that is, the selenium content increased continuously before 12 h, decreased gradually after 12 h, and remained stable after 48 h. more...

Organism:

Medicago sativa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30915

9 Samples

Download data: TXT

(Submitter supplied) To investigate the effect that biological nitrogen fixation will have on plant responses to nitrogen dose at elevated CO2, alfalfa (Medicago sativa) lines were grown at three nitrogen doses and ambient or elevated CO2. Four lines were used in the study, two lines that can form nodules capable of fixing nitrogen (effective lines) and two lines that can not form nodules capable of nitrogen fixation (ineffective lines). more...

Organism:

Medicago sativa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30915

86 Samples

Download data: TXT

(Submitter supplied) We studied the application of transcriptome technology in alfalfa selenium (Se) treatment. Alfalfa had different states after different concentrations of Se treatment. It shows that lower concentration promoted growth and higher concentration produced toxicity. The positive regulatory effects of moderate Se (100 mg / kg) on alfalfa was determined through preliminary experiments, and the gene expression of Alfalfa under this treatment was further analyzed by transcriptome.

Organism:

Medicago sativa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL31126

6 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Medicago sativa

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL25536

30 Samples

Download data

(Submitter supplied) Abstract: In order to understand the expression patterns of miRNAs in alfalfa under alkali stress, small RNA sequencing was performed on alfalfa roots at different time points under alkali stress, and miRNAs were identified and analyzed.

Organism:

Medicago sativa

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL25536

15 Samples

Download data: TXT

(Submitter supplied) Abstract: In order to clarify the response mechanism of alfalfa under alkali stress, the transcriptome of roots was sequenced at different time points after stress and the expression patterns of all genes were analyzed.

Organism:

Medicago sativa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25536

15 Samples

Download data: TXT

(Submitter supplied) We established a regulatory network for melatonin-induced key signaling pathways and functional genes under salt stress.

Organism:

Medicago sativa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30915

12 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Medicago sativa

Type:

Expression profiling by high throughput sequencing; Other

Platforms:

GPL31996 GPL17647

12 Samples

Download data

(Submitter supplied) RNA-seq and Iso-seq reads used used for gene charaterization. Pacbio amplicon sequencing reads used for mutant characterization

Organism:

Medicago sativa

Type:

Other

Platform:

GPL31996

6 Samples

Download data: XLSX

(Submitter supplied) RNA-seq and Iso-seq reads used used for gene charaterization. Pacbio amplicon sequencing reads used for mutant characterization

Organism:

Medicago sativa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17647

6 Samples

Download data: CSV

(Submitter supplied) We conducted field surveys to detect the population density of the most important invasive weed species and their associated virus vectoring aphids in crops grown under high input (HIF) vs low-input (LIF) field conditions, with and without fertilizers and pesticides. The most frequent invasive weed species were Stenactis annua, Erigeron canadensis and Solidago canadensis. These species were hosts predominantly for the aphids Brachycaudus helichrysi and Aulacorthum solani in both management systems. more...

Organism:

Medicago sativa; Erigeron annuus; Zea mays; Solidago canadensis; Solanum tuberosum; Erigeron canadensis

Type:

Expression profiling by high throughput sequencing

4 related Platforms

6 Samples

Download data: TXT

(Submitter supplied) Plant species posses a special set of genes functional only in arbuscular mycorrhizal symbiosis. So, the model plant Medicago truncatula (Jemalong 5) was used for transcriptome comparative analysis while infected with compatible rhizobia Sinorhizobium meliloti (strain 10) and with or without arbuscular mycorrhizal fungus Rhizophagus irregularis (SYM5). Whole shoot and whole root were used for RNA isolation and processed via one of the European certified Affymetrix core labs (http://core.img.cas.cz).

Organism:

Sinorhizobium meliloti; Medicago sativa; Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL4652

12 Samples

Download data: CEL, CHP

(Submitter supplied) Alfalfa (Medicago sativa L.) is a forage legume with significant agricultural value worldwide. MicroRNAs (miRNAs) are key components of post-transcriptional gene regulation and essentially control almost all aspect of plant growth and development. Although miRNAs have been reported from alfalfa but their expression profiles in different tissues and novel miRNAs as well as their targets have not been confirmed in this plant species. more...

Organism:

Medicago sativa

Type:

Non-coding RNA profiling by high throughput sequencing; Other

Platforms:

GPL17647 GPL25536

11 Samples

Download data: TXT

(Submitter supplied) 12plex_medicago_2014_02 - nar nodule vs root transcriptome - which are the genes differentially expressed in alfalfa spontaneous (non rhizobium-infected) nodules vs. control roots? - biological material: aeroponically grown cuttings of a Medicago sativa (alfalfa) accession that produces empty nodules when nitrogen-starved. Samples for transcriptome comparison: isolated NAR nodules (10 days post N-starvation) vs. more...

Organism:

Medicago sativa; Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL17428

8 Samples

Download data: PAIR

(Submitter supplied) affy_infection_medicago - api mutant has been shown to be more resistant to P. palmivora than the A17 wild type (WT) line. In this study, we want to compare both WT and mutant plant responses to the pathogen as well as their respective control transcriptomes in order to identify api-dependent gene networks. Following germination, A17 or api plants grown in vitro on Agar medium were inoculated (or not = controls) with a P. more...

Organism:

Sinorhizobium meliloti; Medicago sativa; Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL4652

12 Samples

Download data: CEL

(Submitter supplied) The array experiment compares gene expression in roots of Medicago truncatula A17 and Medicago truncatula mutant mtpt4-1 colonized with Gigaspora gigantea. This experiment enabled the identification of a plant transcriptional program associated with arbuscule degeneration. The experiment and data are described in Floss et al., Current Biology (in revision).

Organism:

Medicago sativa; Sinorhizobium meliloti; Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL4652

6 Samples

Download data: CEL, CHP

(Submitter supplied) Root gravitropic response involves signal perception, signal transduction, asymetric auxin transport and differential cell elongation in the root tip. However, the regulatory mechanisms remain largely unknown. We used microarray to compare transcript profiles in the root tip samples of wild type and ngr mutant.

Organism:

Sinorhizobium meliloti; Medicago sativa; Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL4652

6 Samples

Download data: CEL, CHP

(Submitter supplied) api mutant has been shown to be more resistant to P. palmivora than the A17 wild type (WT) line. In this study we want to compare both WT and mutant plant responses to the pathogen as well as their respective control transciptomes in order to identify api-dependent gene networks. Following germination, A17 or api plants grown in vitro on Agar medium were inoculated (or not = controls) with a P. palmivora zoospores (10^5/ml) and harvested 16 hours after inoculation. more...

Organism:

Sinorhizobium meliloti; Medicago sativa; Medicago truncatula

Type:

Expression profiling by array

Platform:

GPL4652

12 Samples

Download data: CEL

(Submitter supplied) By comparing transcriptomes of tolerant and intolerant plants of sickle alfalfa subject to intensive animal grazing, we identified pathways involved in nutrient-responsive signaling, light and wound response, cell wall formation, and energy metabolism. In these pathways, grazing suppressed 39 genes, but less severe in the tolerant plant, and activated 5 genes all carrying polymorphisms in their homologous transcripts between the tolerant and intolerant plants. more...

Organism:

Medicago sativa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17647

3 Samples

Download data: TXT

(Submitter supplied) With the growing limitations on arable land, alfalfa (a widely cultivated, low-input forage) is now being selected to extend cultivation into saline lands for low-cost biofeedstock purposes. Here, minerals and transcriptome profiles were compared between two new salinity-tolerant North American alfalfa breeding populations and a more salinity-sensitive Western Canadian alfalfa population grown under hydroponic saline conditions. more...

Organism:

Medicago sativa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22234

27 Samples

Download data: DIFF, FASTA