GEO DataSets

(Submitter supplied) Solventogenic Clostridium species ferment carbohydrates to acetone, butanol and ethanol which are well-known next-generation biofuels. However, repeated subculture of or continuous fermentation by Clostridium often decreases and eventually terminates the solvent production and spore formation, which is a process called strain degeneration. Supplementation of CaCO3 to fermentation medium could partially recover metabolism of degenerated strain by more than 50% increase of cell growth and solvent production. more...

Organism:

Clostridium beijerinckii NCIMB 8052

Type:

Expression profiling by array

Platform:

GPL19469

6 Samples

Download data: TXT

(Submitter supplied) The transcriptome analysis by microarray was applied on wild-type and degenerated strains of C. beijerinckii NCIMB8052 (WT-8052 and DG-8052, respectively) to elucidate its mechanism of degeneration during solvent fermentation. The comparison of gene expression pattern in relation to ABE production is expected to provide insights toward metabolically engineering of C. beijerinckii NCIMB8052 with prevention of degeneration and eventually enhancement of ABE production.

Organism:

Clostridium beijerinckii NCIMB 8052

Type:

Expression profiling by array

Platform:

GPL19469

6 Samples

Download data: TXT

(Submitter supplied) The impacts of ferulic acid, a phenolic compound commonly found in lignin hydrolysates, on the growth, solvent production and transcriptional responses of C. beijerinckii NCIMB 8052 were determined. Addition of ferulic acid to growing cultures resulted in a decrease in the growth and solvent production by 30 and 25%, respectively, when compared to the control cultures. To better understand the toxicity of this compound, microarray analyses were performed using samples taken from these cultures at three different growth states. more...

Organism:

Clostridium beijerinckii NCIMB 8052; Clostridium beijerinckii

Type:

Expression profiling by array

Platform:

GPL19923

16 Samples

Download data: TXT

(Submitter supplied) Furfural is the prevalent microbial inhibitor generated during pretreatment and hydrolysis of lignocellulosic biomass to monomeric sugars, but the molecular response of Clostridium beijerinckii NCIMB 8052 to this compound is unknown. To discern the effect of furfural on C. beijerinckii and to gain insights into the molecular mechanisms of action and detoxification, we studied the physiological changes of furfural-stressed cultures during acetone-butanol-ethanol (ABE) fermentation, and profiled differentially expressed genes by genome-wide transcriptional analysis. more...

Organism:

Clostridium beijerinckii NCIMB 8052

Type:

Expression profiling by array

Platform:

GPL16336

4 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Clostridium beijerinckii NCIMB 8052; Clostridium beijerinckii

Type:

Expression profiling by array

Platform:

GPL6990

21 Samples

Download data: GPR

(Submitter supplied) The fermentation culture of Clostridium beijerinckii mutant BA101 was monitored from exponential growth to stationary phase. During this period the culture underwent a shift from acidogenesis to solventogenesis. Acetone and butanol production was initiated with the onset of the solventogenic phase. Using DNA microarray changes in gene expression were examined during the transitional period.

Organism:

Clostridium beijerinckii; Clostridium beijerinckii NCIMB 8052

Type:

Expression profiling by array

Platform:

GPL6990

11 Samples

Download data: GPR

(Submitter supplied) The Clostridium beijerinckii NCIMB 8052 wild-type culture was monitored from exponential growth to stationary phase. During this period the culture underwent a shift from acidogenesis to solventogenesis. Acetone and butanol production was initiated with the onset of the solventogenic phase. Using DNA microarray changes in gene expression were examined during the transitional period.

Organism:

Clostridium beijerinckii NCIMB 8052

Type:

Expression profiling by array

Platform:

GPL6990

10 Samples

Download data: GPR

(Submitter supplied) see manufacturer's web site at http://www.agilent.com/

Organism:

Clostridium beijerinckii NCIMB 8052

2 Series

12 Samples

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(Submitter supplied) Each array has 30,720 potential addresses for probes, of which 25,015 spots have 45-47mer probes for Clostridium beijerinckii NCIMB 8052 (Cb) genes, 4,985 features have no probe, 608 features have MYcroarray in-house QC probes and 112 features have positive control probes. Each gene is appraised by one unique probe sequence. There are 5 identical replicates of each Cb probe sequence such that a total of 5,003 Cb genes are appraised by each array. more...

Organism:

Clostridium beijerinckii NCIMB 8052

1 Series

4 Samples

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(Submitter supplied) The draft genome sequence assembly of Clostridium beijerinckii (whole genome shotgun [WGS] project accession AALO00000000) was used as a source of the genes used in this study. Note, this WGS project has been superceded by the complete genome in (non-WGS) GenBank record CP000721. The Platform data table reflects a condensed representation of the array's replicate features. The full array layout representing all of the individual features is linked as a supplementary file at the foot of this record. more...

Organism:

Clostridium beijerinckii NCIMB 8052

3 Series

21 Samples

Download data: TXT