GEO DataSets

(Submitter supplied) The MalNO is a putative two-component signal transduction system, previously known as the VirJI sytem. MalO is the putative cognate response regulator of the MalN sensor histidine kinase. Based on previous evidence that suggested the plc gene, encoding α-toxin, was upregulated during stationary phase in the malO mutant, microarrays were used to analyse the transcriptome of a malO mutant during stationary phase growth. more...

Organism:

Clostridium perfringens str. 13

Type:

Expression profiling by array

Platform:

GPL11408

4 Samples

Download data: TXT

(Submitter supplied) ReeS, previously named as CPE1512, was originally annotated as the only hybrid sensor histidine kinase/response regulator in Clostridium perfringens. Further evidence suggests that ReeS is more likely to function as an orphan sensor histidine kinase. A reeS deletion mutant was constructed and the transcriptome analysed using microarrays.

Organism:

Clostridium perfringens str. 13

Type:

Expression profiling by array

Platform:

GPL11408

4 Samples

Download data: TXT

(Submitter supplied) The microarrays were subjected to two-color hybridization using Cy5 and Cy3 dyes incorporated in the cDNAs synthesized from total RNA samples of strains. We compared each gene’s transcription level between the wild-type (WT) strain 13 (labeled by Cy5) and the virX-mutant strain (TS186) (labeled by Cy3) on a single DNA microarray.

Organism:

Clostridium perfringens str. 13

Type:

Expression profiling by array

Platform:

GPL7332

4 Samples

Download data: TXT

(Submitter supplied) RevR is a putative orphan response regulator with a high degree of similarity to YycF from Bacilus subtilis and PhoB from Clostridium kluyveri. A revR deletion mutant of C. perfringens strain 13 was generated and the transcriptome analysed using microarrays.

Organism:

Clostridium perfringens str. 13

Type:

Expression profiling by array

Platform:

GPL11408

4 Samples

Download data: TXT

(Submitter supplied) The microarrays were subjected to two-color hybridization using Cy5 and Cy3 dyes incorporated in the cDNAs synthesized from total RNA samples of strains. We compared each gene’s transcription level between the wild-type (WT) strain 13 (labeled by Cy5) and the VirR-mutant strain (TS133) or VR-RNA-mutant strain (TS140) (both labeled by Cy3) on a single DNA microarray. Keywords: gene regulation in time course

Organism:

Clostridium perfringens str. 13

Type:

Expression profiling by array

Platform:

GPL7332

6 Samples

Download data: TXT

(Submitter supplied) Identical to GPL7332 with the exception that genes encoded on the extra-chromosomal plasmid pCP13 are included on the array Protocol: 2723 genes and 144 intergenic regions of strain 13 were amplified by PCR with gene-specific primers and PCR products were confirmed by gel electrophoresis In total, 2867 probes were spotted onto poly-L-Lysine coated glass slides in twice (SD10011; Matsunami Glass Ind., Osaka, Japan) using SPBIO (Hitachi Software Engineering, Tokyo, Japan).

Organism:

Clostridium perfringens str. 13

3 Series

12 Samples

Download data

(Submitter supplied) 2723 genes and 144 intergenic regions of strain 13 were amplified by PCR with gene-specific primers and PCR products were confirmed by gel electrophoresis In total, 2867 probes were spotted onto poly-L-Lysine coated glass slides in twice (SD10011; Matsunami Glass Ind., Osaka, Japan) using SPBIO (Hitachi Software Engineering, Tokyo, Japan).

Organism:

Clostridium perfringens str. 13

2 Series

10 Samples

Download data