GEO DataSets

(Submitter supplied) We assessed changes in gene expression in response to manganese availability for the human pathogen Corynebacterium diphtheriae. Total RNA was harvested from wild-type C. diphtheriae strain 1737 and an isogenic ΔmntR (dip0619) grown in semi-defined metal-limited media (mPGT) without or with 5 µM manganese chloride supplementation. Three biological replicates were prepared and five genes were assessed by real-time PCR to validate the array results: dip0124, dip0169, dip0615, dip1923, and dip2261. more...

Organism:

Corynebacterium diphtheriae

Type:

Expression profiling by array

Platform:

GPL30087

12 Samples

Download data: TXT

(Submitter supplied) RNA degradation is a crucial process in bacterial cells for maintaining proper transcriptome homeostasis and coping with changing environments. A specialized ribonuclease known as RNase J (RnJ) participates in mRNA turnover in many Gram-positive bacteria; however, nothing is known about this process in Corynebacterium diphtheriae, nor is the identity of this RNase. We report here that C. diphtheriae DIP1463 encodes a predicted RnJ homolog, comprised of an N-terminal beta-lactamase domain, followed by beta-CASP and C-terminal domains. more...

Organism:

Corynebacterium diphtheriae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29654

6 Samples

Download data: XLSX

(Submitter supplied) We assessed changes in gene expression in response to zinc availability for the human pathogen Corynebacterium diphtheriae. Expression profiles of wild-type C. diphtheriae strain 1737 grown in semi-defined metal-poor media (mPGT) without and with 5 µM zinc chloride supplementation were compared; the expression profile of wild-type C. diphtheriae strain 1737 in zinc-replete conditions was also compared against an isogenic Δzur mutant grown in zinc-replete conditions. more...

Organism:

Corynebacterium diphtheriae NCTC 13129; Corynebacterium diphtheriae

Type:

Expression profiling by array

Platform:

GPL25539

9 Samples

Download data: TXT, XLSX

(Submitter supplied) We assessed changes in gene expression in response to iron availability for the human pathogen Corynebacterium diphtheriae. Expression profiles of wild-type C. diphtheriae strain 1737 grown in semi-defined metal-poor media (mPGT) in iron-limiting (0.5 µM iron chloride supplementation) and iron-replete (10 µM supplementation) conditions were compared; the expression profiles of wild-type C. diphtheriae strain 1737 during growth in iron-replete conditions was also compared against an isogenic ΔdtxR mutant grown in iron-replete conditions. more...

Organism:

Corynebacterium diphtheriae NCTC 13129; Corynebacterium diphtheriae

Type:

Expression profiling by array

Platform:

GPL25539

9 Samples

Download data: TXT

(Submitter supplied) Background: The human pathogen Corynebacterium diphtheriae is the causative agent of the disease diphtheria. In the 1990s a large diphtheria outbreak in Eastern Europe was caused by the strain C. diphtheriae NCTC 13129. Although the genome was sequenced several years ago, not much is known about the transcriptome. Our aim was to use RNA Sequencing to close this knowledge gap and gain insights into the transcriptional landscape of C. more...

Organism:

Corynebacterium diphtheriae NCTC 13129

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23376

7 Samples

Download data: XLSX

(Submitter supplied) Arrays of this design have barcodes that begin with 16085697 or 2585697. Features are numbered numbered Left-to-Right, Top-to-Bottom as scanned by an Agilent scanner (barcode on the left, DNA on the back surface, scanned through the glass), matching the FeatureNum output from Agilent's Feature Extraction software. Orientation: Rows and columns are numbered as scanned by an Axon Scanner (barcode on the bottom, DNA on the front surface). more...

Organism:

Corynebacterium diphtheriae

1 Series

12 Samples

Download data: TXT

(Submitter supplied) See manufacturer's web site at http://www.agilent.com/ .

Organism:

Corynebacterium diphtheriae NCTC 13129

2 Series

1 Related Platform

18 Samples

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(Submitter supplied) Arrays of this design have barcodes that begin with 16084856 or 2584856. Orientation: Features are numbered numbered Left-to-Right, Top-to-Bottom as scanned by an Agilent scanner (barcode on the left, DNA on the back surface, scanned through the glass), matching the FeatureNum output from Agilent's Feature Extraction software. The ID column represents the Agilent Feature Extraction feature number. more...

Organism:

Corynebacterium diphtheriae NCTC 13129

1 Related Platform

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