GEO DataSets

(Submitter supplied) Infection caused by bacteria from environmental reservoirs such as E. coli and S. uberis have not decreased in prevalence. Lack of success in controlling bovine mastitis due to S. uberis is associated with the route of infection which is not well understood and there is inadequate information on pathogenesis of S. uberis. Therefore, this study was to investigate the virulence factors of S. uberis using comparative genome analyses using isolates from cows with clinical mastitis and isolates from cows with a low cell count in their milk using a Subtracted Diversity Array (SDA). more...

Organism:

Streptococcus uberis

Type:

Genome variation profiling by array

Platform:

GPL19490

31 Samples

Download data: TXT

(Submitter supplied) Comparison of bacterial strain S. uberis 0140J with an isogenic mutant (same strain) with a lesion 338 basepairs downstream of the ATG start codon of the MtuR or sub0472 gene. Bacteria were grown in stationary culture in THB media at 37°C in the presence of 1mM manganese with triplicate samples of RNA extracted at early exponential (OD550 =0.43) and late exponential (OD550 =0.78) phases of growth. Microarray analysis was performed on custom-made S. more...

Organism:

Streptococcus uberis; Streptococcus uberis 0140J

Type:

Expression profiling by array

Platform:

GPL14778

6 Samples

Download data: TXT

(Submitter supplied) Comparison of bacterial strains S. uberis 0140J with isogenic mutant (same strain) with lesion 5 basepairs upstream of ATG start codon of the vru or sub0144 gene. Bacteria were grown in stationary culture in THB media at 37C with triplicate samples extracted at early exponential (OD550 =0.42) at late exponential (OD550 =0.75) phases of growth Microarray analysis performed on custom made S. uberis 0140J array containing 3780 features of 60mer oligonucleotides in triplicate with two probes for each individual gene

Organism:

Streptococcus uberis; Streptococcus uberis 0140J

Type:

Expression profiling by array

Platform:

GPL14778

6 Samples

Download data: TXT

(Submitter supplied) Streptococcus uberis is one of the principal causative agents of bovine mastitis. The organism is typically considered an environmental pathogen, however, recent studies also suggest possible host adaptation. In this study, two multilocus sequence typing (MLST) schemes and whole genome DNA microarrays were used to evaluate the degree and nature of genome flexibility between S. uberis strains. The 21 isolates examined in this study arise from a collection of 232 international isolates for which previous epidemiological and preliminary genotyping data existed. more...

Organism:

Streptococcus uberis; Streptococcus equi subsp. equi; Streptococcus equi subsp. zooepidemicus

Type:

Genome variation profiling by array

Platforms:

GPL7311 GPL7308

44 Samples

Download data: GPR

(Submitter supplied) Streptococcus uberis isolates from cows with clinical mastitis and belonging to MLST types associated with virulence were selected as the source of genomic tester DNA. Isolates from cows with low cell counts and thought to represent possible non-virulent isolates were selected as the source of genomic driver DNA for suppression subtractive hybridization. All the isolates were collected during a previous study conducted at RMIT laboratory by Tomita et al. more...

Organism:

Streptococcus uberis

1 Series

31 Samples

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(Submitter supplied) DesignID : 024261 Protocol: S. uberis 0140J DNA microarray encompassed the whole bacterial genome, composed of 3780 features, with optimised 60mer oligonucleotide probes printed in triplicate. Two separate probes were designed to match each individual gene determined from the S. uberis 0140J genome annotation with regions of homology discounted. Arrays were printed on glass microscope slides following described protocols (Hughes, Mao et al, Nature Biotechnol 2001, 9(4)342-7).

Organism:

Streptococcus uberis 0140J

2 Series

12 Samples

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(Submitter supplied) This array represents a condensed verion of full array, which is linked as a supplementary file below. Protocol: http://www.combimatrix.com/docs/PTL005_00_4x2K_%20Hyb_%20Imaging.pdf

Organism:

Streptococcus uberis

1 Series

21 Samples

Download data: TXT

(Submitter supplied) This array represents a condensed verion of full array, which is linked as a supplementary file below. Protocol: http://www.combimatrix.com/docs/PTL005_00_4x2K_%20Hyb_%20Imaging.pdf

Organism:

Streptococcus uberis

1 Series

23 Samples

Download data: TXT