txid129951[Organism] - GEO DataSets

(Submitter supplied) We recently reported that serine-arginine-rich (SR) protein-mediated pre-mRNA structural remodeling generates a pre-mRNA 3D structural scaffold that is stably recognized by the early spliceosomal components. However, the intermediate steps between the free pre-mRNA and the assembled early spliceosome are not yet characterized. By probing the early spliceosomal complexes in vitro and RNA-protein interactions in vivo, we show that the SR proteins bind the pre-mRNAs cooperatively generating a substrate that recruits U1 snRNP and U2AF65 in a splice signal-independent manner. more...

Organism:: Human adenovirus 2

Type:: Other

Platform:: GPL30034
22 Samples

Download data: SHAPE, TXT

Series

Accession:: GSE188652

ID:: 200188652

PubMed Full text in PMC Similar studies

Select item 2001731785.

Discovery of pre-mRNA structural scaffold as a contributor to mammalian splicing code

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Human adenovirus 2

Type:: Other

Platform:: GPL30034
59 Samples

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Series

Accession:: GSE173178

ID:: 200173178

PubMed Full text in PMC Similar studies

Select item 2001731776.

Discovery of pre-mRNA structural scaffold as a contributor to mammalian splicing code [In Vivo]

(Submitter supplied) The specific recognition of splice signals at or near the exon-intron junctions is not explained by their weak conservation across the mammalian transcriptome and postulated to require a multitude of features embedded in the pre-mRNA strand. We explored the possibility of three-dimensional structural scaffold of a pre-mRNA guiding early spliceosomal components to the splice signal sequences. We find that mutation in non-cognate splice signal sequences of a model pre-mRNA substrate could impede recruitment of early spliceosomal components due to disruption of global structure of the pre-mRNA. more...

Organism:: Human adenovirus 2

Type:: Other

Platform:: GPL30034
9 Samples

Download data: SHAPE

Series

Accession:: GSE173177

ID:: 200173177

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2001731757.

Discovery of a pre-mRNA structural scaffold as a contributor to the mammalian splicing code [in vitro]

(Submitter supplied) The specific recognition of splice signals at or near exon-intron junctions is not explained by their weak conservation and instead is postulated to require a multitude of features embedded in the pre-mRNA strand. We explored the possibility of three-dimensional structural scaffold of AdML – a model pre-mRNA substrate – guiding early spliceosomal components to the splice signal sequences. We find that mutations in the non-cognate splice signal sequences impede recruitment of early spliceosomal components due to disruption of the global structure of the pre-mRNA. more...

Organism:: Human adenovirus 2

Type:: Other

Platform:: GPL30034
50 Samples

Download data: SHAPE

Series

Accession:: GSE173175

ID:: 200173175

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2001040588.

Clearance of a persistent picornavirus infection is associated with enhanced pro-apoptotic and cellular immune responses

(Submitter supplied) Immunomodulatory mechanisms associated with clearance versus persistence of foot-and-mouth disease virus (FMDV) in micro-dissected compartments of the bovine nasopharynx were investigated using qRT-PCR and whole transcriptome microarray. Analysis of tissue samples obtained by laser capture microdissection (LCM) during transitional and persistent phases of infection demonstrated significant differences in transcriptome profiles of animals that cleared infection versus those that became persistently infected carriers. more...

Organism:: Foot-and-mouth disease virus; Human adenovirus 5; Bos taurus

Type:: Expression profiling by array

Platform:: GPL22061
32 Samples

Download data: GPR

Series

Accession:: GSE104058

ID:: 200104058

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 2000835149.

Transcriptomic Analysis of Persistent Infection with Foot-and-Mouth Disease Virus in Cattle Suggests Impairment of Apoptosis and Cell-Mediated Immunity in the Nasopharynx

(Submitter supplied) In order to investigate the mechanisms of persistent foot-and-mouth disease virus (FMDV) infection in cattle, transcriptome alterations associated with the FMDV carrier state were characterized using a bovine whole-transcriptome microarray. Eighteen cattle (8 vaccinated with a recombinant FMDV A vaccine, 10 non-vaccinated) were challenged with FMDV A24 Cruzeiro, and the gene expression profiles of nasopharyngeal tissues collected between 21 and 35 days after challenge were compared between 11 persistently infected carriers and 7 non-carriers. more...