GEO DataSets

Analysis of chimpanzee iPSCs generated from blood cells using Sendai virus vector TS12KOS which carries reprogramming factors KLF4, OCT3/4, and SOX2. TS12KOS was also used to establish human iPSCs from fibroblasts. Results examine differentially expressed genes between human and chimpanzee iPSCs.

Organism:

Pan troglodytes; Homo sapiens

Type:

Expression profiling by array, transformed count, 2 cell type, 5 individual, 2 species sets

Platform:

GPL570

Series:

GSE62572

7 Samples

Download data: CEL

(Submitter supplied) Gene expression study of human and Chimpanzee iPS cell.

Organism:

Homo sapiens; Pan troglodytes

Type:

Expression profiling by array

Dataset:

GDS5443

Platform:

GPL570

7 Samples

Download data: CEL

(Submitter supplied) We investigated that gene expression profile of generated human iPS cells from cord blood cells using temperature sensitive sendai-virus vector.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

3 Samples

Download data: CEL, CHP

(Submitter supplied) Whole-genome expression analysis of non-integrating Sendai virus (SeV) vector and retroviral vector-generated human iPS cells.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4133

19 Samples

Download data: TXT

(Submitter supplied) We introduce a method for generating transgene-free and high-quality naive human induced pluripotent stem cells (iPSCs) using a modified Sendai virus (SeV) vector reprogramming system. This reprogramming method realizes the derivation of naive iPSCs from various somatic cells accompanied with fast and robust SeV vector removal at early passages. The established naive iPSCs have superior differentiation ability compared with iPSCs derived from conventional methods.

Organism:

Homo sapiens

Type:

Genome variation profiling by SNP array

Platform:

GPL32421

11 Samples

Download data: IDAT, TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Methylation profiling by array; Expression profiling by high throughput sequencing

4 related Platforms

26 Samples

Download data: IDAT, TXT

(Submitter supplied) We introduce a method for generating transgene-free and high-quality naive human induced pluripotent stem cells (iPSCs) using a modified Sendai virus (SeV) vector reprogramming system. This reprogramming method realizes the derivation of naive iPSCs from various somatic cells accompanied with fast and robust SeV vector removal at early passages. The established naive iPSCs have superior naive-specific differentiation ability compared with iPSCs derived from conventional methods.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL18573 GPL16791

13 Samples

Download data: TXT

(Submitter supplied) We introduce a method for generating transgene-free and high-quality naive human induced pluripotent stem cells (iPSCs) using a modified Sendai virus (SeV) vector reprogramming system. This reprogramming method realizes the derivation of naive iPSCs from various somatic cells accompanied with fast and robust SeV vector removal at early passages. The established naive iPSCs have superior naive-specific differentiation ability compared with iPSCs derived from conventional methods.

Organism:

Homo sapiens

Type:

Methylation profiling by array

Platforms:

GPL21145 GPL13534

13 Samples

Download data: IDAT, TXT

(Submitter supplied) A variety of somatic cells can be reprogrammed to induced pluripotent stem cells (iPSCs), but the small number of CD34+ hematopoietic stem cells (HSCs) present in non-mobilized peripheral blood (PB) would be a convenient and desirable starting target. We report here a simple method for targeting derivation of iPSC from non-mobilized PB CD34+ HSCs using immunobead purification and 2-4 day culture to achieve enrichment of CD34+ HSCs to 80±9%, followed by reprogramming transduction with loxP-flanked polycistronic (Oct4, Klf4, Sox2, and c-Myc) STEMCCA-loxP lentivector at an MOI of 2. more...

Organism:

Homo sapiens

Type:

Methylation profiling by genome tiling array

Platform:

GPL13534

21 Samples

Download data: TXT

(Submitter supplied) γδT cells constitute a small proportion of lymphocytes in peripheral blood. Unlike αβT cells, the anti-tumor activities are exerted through several different pathways in a MHC-unrestricted manner. Thus, immunotherapy using γδT cells is considered to be effective for various types of cancer. Occasionally, however, ex vivo expanded cells are not as effective as expected due to cell exhaustion. To overcome the issue of T-cell exhaustion, researchers have generated induced pluripotent stem cells (iPSCs) that harbor the same T-cell receptor (TCR) genes as their original T-cells, which provide nearly limitless sources for antigen-specific cytotoxic T lymphocytes (CTLs). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL15207

3 Samples

Download data: CEL

(Submitter supplied) Induced pluripotent stem cells (iPSCs) can be derived from somatic cells by the introduction of the transcription factors Oct4, Sox2, Klf4 and cMyc using various methods. Here, we describe a new approach for the derivation of murine iPSCs using a polycistronic non-viral inducible vector integrated into pseudo attP sites via the C31 integrase-mediated site-specific recombination and subsequent vector excision by Cre recombinase. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6885

15 Samples

Download data: TXT

(Submitter supplied) Emergence of induced pluripotent stem cells (iPSC) technology has paved novel routes for regenerative medicine. iPSCs offer the possibilities of disease modeling, drug toxicity studies as well as cell replacement therapies by autologous transplantation. Classical protocols of iPSC generation harness infection by retro- or lenti-viruses. Although such integrating viruses represent very robust tools for reprogramming, the presence of viral transgenes in iPSCs is deleterious as it holds the risk of insertional mutagenesis leading to malignant transformation. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

10 Samples

Download data: TXT

(Submitter supplied) Measles virus vector expressing the 4 reprogramming factors, OCT4, SOX2, KLF4 and cMYC was produced and used to derived iPSC from neonatal human fibroblasts (BJ). We used microarrays to compare the global gene expression in the derived MV-iPSC and compare it to the parental human neonatal fibroblast (BJ) and human embryonic stem cell (GSM551202)

Organism:

Homo sapiens

Type:

Expression profiling by array; Third-party reanalysis

Platform:

GPL570

4 Samples

Download data: CEL, TXT

(Submitter supplied) Transcriptional analysis was performed on pre and post excision human induced pluripotent stem cells, the donor human dermal fibroblasts (HDFs) they were derived from and control human embryonic stem cells

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

8 Samples

Download data: CEL

(Submitter supplied) This experiment was designed to show the similarity among TiPS cells generated in a difined condition and ES cells and the differenced between TiPS cells and T cells.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13497

4 Samples

Download data: TXT

(Submitter supplied) Macrophages are important target cells for diverse viruses, and thus represent a valuable model system for studying virus biology. Isolation of primary human macrophages is done by culture of dissociated tissues or from differentiated blood monocytes, but these methods are both time consuming and result in low numbers of recovered macrophages. Here, we explore whether macrophages derived from human induced pluripotent stem cells (iPSCs)—which proliferate indefinitely and potentially provide unlimited starting material— could serve as a faithful model system for studying virus biology. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL24676

28 Samples

Download data: CSV, GTF

(Submitter supplied) Comparative genomics studies in primates are extremely restricted due to our limited access to samples from non-human apes. In order to gain better insight into the genetic processes that underlie variation in complex phenotypes in primates, we must have access to faithful model systems for a wide range of cell types. To facilitate this, we have generated a panel of 7 fully characterized chimpanzee induced pluripotent stem cell (iPSC) lines derived from healthy donors. more...

Organism:

Pan troglodytes; Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing; Third-party reanalysis

Platform:

GPL19148

9 Samples

Download data: BED, TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Pan troglodytes; Homo sapiens

Type:

Expression profiling by high throughput sequencing; Methylation profiling by array; Genome binding/occupancy profiling by high throughput sequencing; Third-party reanalysis

Platforms:

GPL16791 GPL13534 GPL19148

65 Samples

Download data: BED

(Submitter supplied) Comparative studies in primates are extremely restricted because we only have access to a few types of cell lines from non-human apes and to a limited collection of frozen tissues. In order to gain better insight into regulatory processes that underlie variation in complex phenotypes, we must have access to faithful model systems for a wide range of tissues and cell types. To facilitate this, we have generated a panel of 7 fully characterized chimpanzee (Pan troglodytes) induced pluripotent stem cell (iPSC) lines derived from fibroblasts of healthy donors. more...

Organism:

Homo sapiens; Pan troglodytes

Type:

Methylation profiling by array

Platform:

GPL13534

28 Samples

Download data: TXT

(Submitter supplied) Comparative studies in primates are extremely restricted because we only have access to a few types of cell lines from non-human apes and to a limited collection of frozen tissues. In order to gain better insight into regulatory processes that underlie variation in complex phenotypes, we must have access to faithful model systems for a wide range of tissues and cell types. To facilitate this, we have generated a panel of 7 fully characterized chimpanzee (Pan troglodytes) induced pluripotent stem cell (iPSC) lines derived from fibroblasts of healthy donors. more...

Organism:

Pan troglodytes; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL16791 GPL19148

28 Samples

Download data: TXT