GEO DataSets

Analysis of pseudopodia from NIH/3T3 cells extending protrusions in response to fibronectin, a haptotactic stimulus. Asymmetric distribution of RNAs establishes polarity in multiple cell types. Results provide insight into RNAs that localize in cell protrusions in response to migratory stimuli.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 other sets

Platform:

GPL1261

Series:

GSE10227

4 Samples

Download data: CEL, EXP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

8 Samples

Download data: CEL, EXP

(Submitter supplied) The goal of the study was to identify on a genome-wide scale RNAs that are enriched at the leading edge of migrating cells. For this, we employed a fractionation method in which cells are plated on a microporous filter whose bottom side only is coated with fibronectin. The cells thus polarize and extend pseudopodial protrusions towards the bottom surface. These protruding pseudopodia can then be physically isolated from the bottom surface of the filter and their contents compared with the remaining cell bodies, which are isolated from the upper surface of the filter. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3288

Platform:

GPL1261

4 Samples

Download data: CEL, EXP

(Submitter supplied) The goal of the study was to identify on a genome-wide scale RNAs that are enriched at the leading edge of migrating cells. For this, we employed a fractionation method in which serum-starved cells are first plated and allowed to spread on a microporous filter. Addition of LPA at the bottom side of the filter induces the cells to polarize and extend pseudopodial protrusions. These protruding pseudopodia can then be physically isolated from the bottom surface of the filter and their contents compared with the remaining cell bodies, which are isolated from the upper surface of the filter. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3287

Platform:

GPL1261

4 Samples

Download data: CEL, EXP

Analysis of pseudopodia from NIH/3T3 cells extending protrusions in response to LPA, a chemotactic stimulus. Asymmetric distribution of RNAs establishes polarity in multiple cell types. Results provide insight into RNAs that localize in cell protrusions in response to migratory stimuli.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 other sets

Platform:

GPL1261

Series:

GSE10226

4 Samples

Download data: CEL, EXP

(Submitter supplied) We generated fibroblast lines overexpressing beta-globin constructs under a doxycycline-inducible promoter. These constructs contain the beta-globin coding sequence followed by either a control 3'UTR (HBB) or the Pkp4 3'UTR. The Pkp4 3'UTR carries a protrusion-targeting element and is expected to compete with localization of endogenous protrusion-localized mRNAs. We used a fractionation scheme to isolate protrusions and cell bodies from HBB and Pkp4 UTR-expressing fibroblasts, which were induced to extend protrusions in response to addition of lysophosphatidic acid (LPA). more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

16 Samples

Download data: TXT

(Submitter supplied) We used a fractionation scheme to isolate protrusions and cell bodies from control or APC knockdown fibroblasts, which were induced to extend protrusions in response to addition of lysophosphatidic acid (LPA). Four replicate protrusion and cell body samples for each cell type were analyzed by RNA-Seq. To identify RNAs enriched in protrusions, a protrusion/cell body ratio was calculated. We find that ca. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

16 Samples

Download data: TXT

(Submitter supplied) Transcriptome analysis of specific mRNA interacting with KIF1C-GFP in HeLa Cells. RNA localization and local translation are involved in a wide range of cellular functions. In mammals, a class of mRNAs localize to cytoplasmic protrusions in an APC dependent manner, with important roles during cell migration. Here, we investigated this localization mechanism. We found that the KIF1C motor interacts with many APC-dependent mRNAs and is required for their localization. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17586

4 Samples

Download data: CEL, XLSX

(Submitter supplied) To assay the relative localization of RNA molecules in the apical and basal compartments of human intestinal epithelial cells, we performed Halo-seq using apically and basally localized HaloTag bait proteins.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

18 Samples

Download data: SF