GEO DataSets

Analysis of skeletal muscles from normal, healthy glucose-tolerant individuals exposed to acute physiological hyperinsulinemia for up to 4 hours. Results suggest a low-grade inflammatory response which may provide insight into the etiology of insulin resistance in type 2 diabetes and obesity.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 12 individual, 3 time sets

Platform:

GPL96

Series:

GSE9105

36 Samples

Download data: CEL

(Submitter supplied) This study was undertaken to test the hypothesis that short term exposure (4 hours) to physiologic hyperinsulinemia in normal, healthy subjects without a family history of diabetes would induce a low grade inflammatory response, independently of glycemic status. We performed euglycemic hyperinsulinemic (80 mU/m2/min) clamps in 12 healthy, insulin sensitive subjects with no family history of diabetes followed by biopsies of the vastus lateralis muscle taken basally and after 30 and 240 minutes of insulin infusion. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3181

Platform:

GPL96

36 Samples

Download data: CEL

(Submitter supplied) Insulin action in target tissues involved precise regulation of gene expression. To define the set of insulin-regulated genes in human skeletal muscle, we analyzed the global changes in mRNA levels during a 3-h hyperinsulinemic euglycemic clamp in vastus lateralis muscle of six healthy subjects. Using 29,308 cDNA element microarrays, we found that the mRNA expression of 762 genes, including 353 expressed sequence tags, was significantly modified during insulin infusion. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL3271

6 Samples

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(Submitter supplied) Insulin is a potent pleiotropic hormone that affects processes such as cellular growth, differentiation, apoptosis, ion flux, energy expenditure, and carbohydrate, lipid, and protein metabolism. We used microarrays to detail the global programme of gene expression underlying the influence of insulin in human skeletal muscle collected from different human individuals including 20 insulin sensitive, 20 insulin resistant and 15 diabetic patients. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3715

Platform:

GPL91

110 Samples

Download data: CEL, TXT

Analysis of vastus lateralis muscle biopsies from insulin-sensitive subjects, insulin-resistant subjects, and diabetic patients, following insulin treatment. Results provide insight into the molecular basis of insulin action in skeletal muscle and the underlying defects causing insulin resistance.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent, 3 disease state sets

Platform:

GPL91

Series:

GSE22309

110 Samples

Download data: CEL

(Submitter supplied) Hyperinsulinemia is often viewed as compensatory to insulin resistance, but studies have shown that high levels of insulin may contribute to insulin resistance. The precise mechanisms by which hyperinsulinemia contributes to insulin resistance remain poorly defined. To understand direct effects of prolonged exposure to excess insulin in muscle cells, we incubated differentiated C2C12 myotubes with elevated insulin for 16 hours, followed by 6 hours serum starvation, before examining key insulin signaling nodes. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

20 Samples

Download data: TXT

(Submitter supplied) 6 lean humans were submitted to a 3 hours hyperinsulinemic-euglycemic clamp. Skeletal muscle biopsies were taken before and after the clamp. Set of arrays that are part of repeated experiments

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6991

6 Samples

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(Submitter supplied) Retinoid X receptor (RXR)-gamma is a nuclear receptor-type transcription factor expressed mostly in the skeletal muscle, and regulated by nutritional conditions. Previously, we established transgenic mice overexpressing RXR-gamma in the skeletal muscle (RXR-gamma mice), which showed lower blood glucose than the control mice. We used microarrays to investigate their glucose metabolism gene expression change.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

2 Samples

Download data: CEL

(Submitter supplied) The accumulation of intramyocellular lipid (IMCL) is recognized as an important determinant of insulin resistance, and is increased by a high-fat diet (HFD). However, the effects of HFD on IMCL and insulin sensitivity are highly variable. We used microarrays to detail to identify the genes in skeletal muscle that are related to inter-individual variation of the effects with HFD on the accumulation of intramyocellular lipid and insulin sensitivity.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

20 Samples

Download data: CEL