GEO DataSets

Analysis of mmi1 mutant cells. Mmi1 binds to meiosis-specific mRNAs that contain the DSR region. DSR is a cis-acting element that allows selective removal of the meiosis-specific mRNAs during vegetative growth. Results provide insight into the molecular regulation of meiosis.

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by array, log ratio, 2 genotype/variation sets

Platform:

GPL2849

Series:

GSE3314

4 Samples

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(Submitter supplied) Compared to mitosis, much remains to be elucidated about molecular regulation of meiosis, although increasing light has been shed recently on its signal transduction, cell cycle regulation and chromosome dynamics. Here we show that some transcripts required exclusively for meiosis in fission yeast, such as mei4 mRNA encoding a transcription factor or rec8 mRNA encoding a cohesin subunit, are highly unstable if expressed during the mitotic cell cycle. more...

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by array

Dataset:

GDS3014

Platform:

GPL2849

4 Samples

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(Submitter supplied) We used RIP-CHIP to determine the RNAs bound specifically to RNA binding protein Mei2 in early meiosis in fission yeast.

Organism:

Schizosaccharomyces pombe

Type:

Other

Platform:

GPL24985

2 Samples

Download data: TXT

(Submitter supplied) We used CLIP-Seq to determine the RNAs bound specifically to RNA binding protein Mei2 in early meiosis in fission yeast. We added a TAP tag to the C-terminal ends of two meiotic RNA binding proteins, Mei2 and Msa1. We used an untagged fission yeast strain as a negative control. These strains were nitrogen starved and allowed to progress into meiosis, after which they were harvested, lyzed and crosslinking immunoprecipitaion was performed. more...

Organism:

Schizosaccharomyces pombe

Type:

Other

Platform:

GPL16192

5 Samples

Download data: BW, XLSX

(Submitter supplied) We determined the repertoire of transcripts simultaneously bound by Mmi1 and Mei2 through sequential immunoprecipitations followed by high-throughput sequencing of associated RNAs (seqRIP-seq)

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20584

8 Samples

Download data: TXT

(Submitter supplied) Transcriptome analyses of wild type, mot2∆, mamRNA∆ and mot2∆ mamRNA∆ strains. We determined the role of mamRNA in the accumulation of Mmi1-targeted transcripts observed in mot2∆ cells.

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23689

8 Samples

Download data: TXT

(Submitter supplied) In fission yeast, meiosis-specific transcripts are selectively eliminated during vegetative growth by the combined action of the YTH-family RNA-binding protein Mmi1 and the nuclear exosome. Upon nutritional starvation, the master regulator of meiosis Mei2 inactivates Mmi1, thereby allowing expression of the meiotic program. Here, we show that the E3 ubiquitin ligase subunit Not4/Mot2 of the evolutionarily conserved Ccr4-Not complex, which associates with Mmi1, promotes suppression of meiotic transcripts expression in mitotic cells. more...

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20841

7 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by genome tiling array

Platforms:

GPL16192 GPL6503

21 Samples

Download data: FPKM_TRACKING, TXT

(Submitter supplied) We report gene expression profiling in the fission yeast Schizosaccharomyces pombe. We performed high-throughput sequencing of RNA isolated from wild-type, erh1∆, and ccr4∆ strains. We find that many meiotic gene containing degradation sequence DSR are expressed in vegetative erh1∆, while these meiotic mRNAs do not increase in ccr4∆, indicating that Erh1 and Ccr4 target different set of genes during vegetative growth.

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16192

5 Samples

Download data: FPKM_TRACKING

(Submitter supplied) ChIP-chip analyses of H3K9me2 (in WT, erh1∆, mmi1∆ and ccr4∆), Erh1-GFP (in WT and mmi1∆) and CFP-Mmi1 (in WT)

Organism:

Schizosaccharomyces pombe

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL6503

16 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Schizosaccharomyces pombe

Type:

Genome binding/occupancy profiling by genome tiling array; Expression profiling by high throughput sequencing

Platforms:

GPL16192 GPL6503

23 Samples

Download data: FPKM_TRACKING, TXT

(Submitter supplied) We report gene expression profiling in the fission yeast Schizosaccharomyces pombe. We performed high-throughput sequencing of RNA isolated from wild-type, ago1d, mmi1d and dcr1d strains.

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16192

4 Samples

Download data: FPKM_TRACKING

(Submitter supplied) Occupancy profiling of lysine 9 dimethylated histone H3 in fission yeast. Occupancy profiling of Psc3, Rec8, Rad21 and Rec11 proteins in fission yeast.

Organism:

Schizosaccharomyces pombe

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL6503

19 Samples

Download data: TXT

(Submitter supplied) We report gene expression profiling in the fission yeast Schizosaccharomyces pombe. We performed high-throughput sequencing of RNA isolated from wild-type, mmi1(W112A) mutant, erh1∆, and mmi1∆ strains. We find that mmi1(W112A) and erh1∆ derepress a shared subset of mmi1-repressed genes during vegetative growth.

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20584

8 Samples

Download data: FPKM_TRACKING

(Submitter supplied) Genome wide map of H3K9me2 in 4 different strains of fission yeast Schizosaccharomyces pombe.

Organism:

Schizosaccharomyces pombe

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL20584

6 Samples

Download data: BW

(Submitter supplied) Investigation of whole genome gene expression level changes in red1∆, compared to the wild-type strain.

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by array

Platform:

GPL10720

4 Samples

Download data: TXT

(Submitter supplied) Occupancy profiling of lysine 9 dimethylated histone H3 in fission yeast.

Organism:

Schizosaccharomyces pombe

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL6503

5 Samples

Download data: TXT

(Submitter supplied) In eukaryotic cells, inefficient splicing is surprisingly common and leads to degradation of transcripts with retained introns. How pre-mRNAs are committed to nuclear decay is unknown. Here we uncover a mechanism by which intronic transcripts are targeted for nuclear degradation in fission yeast. Surprisingly, sequence elements within “suicidal” introns co-transcriptionally recruit the exosome adaptor Mmi1 not only to degrade unspliced precursor, but also to downregulate levels of the resulting mRNA. more...

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing; Other

Platforms:

GPL16192 GPL17225

10 Samples

Download data: GTF

(Submitter supplied) The meiotic cohesin Rec8 is required for the stepwise segregation of chromosomes during the two rounds of meiotic division. By directly measuring chromosome compaction in living cells of the fission yeast Schizosaccharomyces pombe, we found an additional role for the meiotic cohesin in the compaction of chromosomes during meiotic prophase. In the absence of Rec8, chromosomes were decompacted relative to those of wild-type cells. more...

Organism:

Schizosaccharomyces pombe

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL1281

4 Samples

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(Submitter supplied) To determine the range of genes whose expression is regulated by CTDK-I under nitrogen starvation, comparison of gene expression profiles between a wild-type strain and a deletion mutant of lsg1, which encodes the gamma subunit of CTDK-I.

Organism:

Schizosaccharomyces pombe

Type:

Expression profiling by array

Platform:

GPL14642

2 Samples

Download data: PAIR, TXT