GEO DataSets

Comparison of CD133+ and CD133- glioblastoma-derived cancer stem cells (CSC). Both CSC subpopulations were derived from primary glioblastomas. Results provide insight into the role of CD133+ and CD133- CSCs in the pathogenesis of glioblastoma.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 cell line sets

Platform:

GPL570

Series:

GSE7181

6 Samples

Download data: CEL, CHP

(Submitter supplied) We cultured tumor cells from 22 GBM under medium conditions favoring the growth of neural stem cells. 11 out of 15 primary GBM contained a significant CD133+ subpopulation that comprised cells showing all hallmarks of neural stem cells. Cell lines derived from these CD133+ GBM showed a neurosphere-like, non-adherent growth pattern. In contrast, 4 out of 15 cell lines derived from primary GBM grew adherent in vitro and were driven by CD133- tumor cells that fulfilled stem cell criteria. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2728

Platform:

GPL570

6 Samples

Download data: CEL, CHP

(Submitter supplied) We compared a large panel of human glioblastoma stem-like (GS) cell lines, corresponding primary tumors and conventional glioma cell lines to identify cell lines that preserve the transcriptome of human glioblastomas most closely, thereby allowing identification of shared therapeutic targets. We used Affymetrix HG-U133 Plus 2.0 microarrays to compare human glioblastoma stem-like (GS) cell lines, corresponding primary tumors and conventional glioma cell lines.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3885

Platform:

GPL570

92 Samples

Download data: CEL, EXP

(Submitter supplied) Recent studies demonstrated that tumor cells with stem cell-like properties can be cultured from human glioblastomas by using conditions that select for the expansion of neural stem cells. We established glioblastoma stem-like (GS-) cell cultures from 9 different glioblastomas, 8 of which generated stably expandable cell lines. Analyzing GS-cell cultures, we discovered two clearly discernable phenotypes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

17 Samples

Download data: CEL, EXP

Analysis of glioblastoma stem-like (GS) cell lines, corresponding glioblastoma primary tumors, conventional glioma cell lines, and GS neurospheres. Results provide insight into cell lines recapitulating transcriptional aspects of glioblastomas, thereby allowing identification of therapeutic targets.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 7 specimen sets

Platform:

GPL570

Series:

GSE23806

92 Samples

Download data: CEL, EXP

(Submitter supplied) Background: Gliomas are the most common type of primary brain tumours, and in this group glioblastomas (GBMs) are the higher-grade gliomas with fast progression and unfortunate prognosis. Two major aspects of glioma biology that contributes to its awful prognosis are the formation of new blood vessels through the process of angiogenesis and the invasion of glioma cells. Despite of advances, two-year survival for GBM patients with optimal therapy is less than 30%. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

16 Samples

Download data: CEL, CHP, TXT

(Submitter supplied) The neural stem cell marker CD133 is reported to identify cells within glioblastoma (GBM) that can initiate neurosphere growth and tumor formation, however, instances of CD133- cells exhibiting similar properties have also been reported. Here, we show that some PTEN-deficient GBM tumors produce a series of CD133+ and CD133- self-renewing tumor-initiating cell types and provide evidence that these cell types constitute a lineage hierarchy. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by SNP array; SNP genotyping by SNP array; Genome variation profiling by genome tiling array; Expression profiling by array

5 related Platforms

171 Samples

Download data: CEL, TXT

(Submitter supplied) This study compared the gene expression change of glioblastoma stem-like cells before and after retinoic acid treatment

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL5175

12 Samples

Download data: CEL, TXT

(Submitter supplied) We use gene expression data to provide a three-faceted analysis on the links between molecular subclasses of glioblastima, epithelial-to mesenchymal transition (EMT) and CD133 cell surface protein. The contribution of this paper is three-folded: First, we used a newly identified signature for epithelial-to-mesenchymal transition in human mammary epithelial cells, and demonstrated that genes in this signature have significant overlap with genes differentially expressed in all known GBM subtypes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

8 Samples

Download data: CEL

(Submitter supplied) Epithelial ovarian cancer is the leading cause of death among gynecologic malignancies. Diagnosis usually occurs after metastatic spread, largely reflecting vague symptoms of early disease combined with lack of an effective screening strategy. Epigenetic mechanisms of gene regulation, including DNA methylation, are fundamental to normal cellular function and also play a major role in carcinogenesis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL3921

95 Samples

Download data: CEL

(Submitter supplied) CD133 (Prominin-1) is considered the most important cancer stem cell (CSC)-associated marker identified so far, with increased expression in the CSC fraction of a large variety of human malignancies, including melanoma. Here we investigated the effects of CD133 down-regulation in vitro and in vivo in human metastatic melanoma. The average number of CD133 molecules on the cell surface of FEMX-I melanoma cells was decreased by 8.7-fold and 1.8-fold using two different short hairpin RNAs. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3489

Platform:

GPL7430

4 Samples

Download data: GPR

Analysis of metastatic melanoma FEMX-I cells depleted for CD133. CD133 is a cancer stem cell-associated antigen expressed in many malignancies such as melanoma. Results provide insight into the role of CD133 in cancer progression.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 protocol sets

Platform:

GPL7430

Series:

GSE13144

4 Samples

Download data: GPR

(Submitter supplied) Several novel potential oncogenic and tumor suppressor miRNAs were identified by using the appropriate controls for stem cells. Expression profiles for human miRNAs in six samples were generated.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL8227

6 Samples

Download data: TXT

(Submitter supplied) As many other tumors, a subset of gliobastoma is thought to be maintained by a restricted population of cancer cells, stem-like cells that express CD133 transmembrane protein. Expression levels of CD133 gene has been linked to a poor prognostic molecular subgroup and is not overexpressed by the PDGF-driven proneural group. Thus, the significance of CD133+ cells for gliomagenesis of the proneural group is undetermined. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

2 Samples

Download data: CEL, CHP

(Submitter supplied) Background: Large-scale genomic analyses of patient cohorts have revealed extensive heterogeneity between individual tumors, contributing to treatment failure and drug resistance. In malignant melanoma, heterogeneity is thought to arise as a consequence of the differentiation of melanoma-initiating cells that are defined by cell-surface markers like CD271 or CD133. Results: Here we identified the nerve growth factor receptor (CD271) as a crucial determinant of melanoma cell tumorigenicity, stem-like properties, heterogeneity and plasticity. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

9 Samples

Download data: TXT

(Submitter supplied) Induction of the transcription factor Sox2 from a doxycycline-inducible promoter in iSox2-DAOY medulloblastoma cells. Affymetrix microarrays were used to characterize the gene expression profile of iSox2-DAOY cells in the absence and presence of doxycycline.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

2 Samples

Download data: CEL, CHP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array; Genome variation profiling by array; Genome variation profiling by genome tiling array

Platforms:

GPL5175 GPL8736 GPL5477

41 Samples

Download data: CEL, CHP, TXT

(Submitter supplied) Transcriptome analysis of RNAs from brain tumor Investigate potential of true exon level expression profiling .

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL5175

16 Samples

Download data: CEL, CHP

(Submitter supplied) GBM is a heterogenous tumor. Based on membrane protein expression, the GBM single cell dissociates were seperated into different subfractions by FACS assay. The genomic aberration among each populations were compared by analysis of CGH data.

Organism:

Homo sapiens

Type:

Genome variation profiling by array

Platform:

GPL5477

14 Samples

Download data: TXT

(Submitter supplied) Examine the genetic abnormality in brain tumors

Organism:

Homo sapiens

Type:

Genome variation profiling by genome tiling array

Platform:

GPL8736

11 Samples

Download data: TXT