GEO DataSets

Analysis of cDNA libraries each generated from a single cultured epidermal stem cell (SC) or transit-amplifying (TA) cell. TA cells are SC progenies that are destined to terminally differentiate. Single cell expression profiling avoids the problem of potentially masking cellular heterogeneity.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 cell type sets

Platform:

GPL96

Series:

GSE4858

12 Samples

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(Submitter supplied) To explore which signaling pathways are of importance in maintenance of human epidermal keratinocytes we have established a protocol which allows single cell expression profiling. The protocol was applied to cultured human epidermal keratinocytes, and single cell cDNA libraries from stem and transit amplifying cells were identified based on the expression of known SC markers, MCSP and Dll1. We used microarrays to obtain expression profiles from single cells and identify common denominators that could be important for maintenance and signalling in epidermal stem cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS2395 GDS2396

Platforms:

GPL96 GPL97

24 Samples

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Analysis of cDNA libraries each generated from a single cultured epidermal stem cell (SC) or transit-amplifying (TA) cell. TA cells are SC progenies that are destined to terminally differentiate. Single cell expression profiling avoids the problem of potentially masking cellular heterogeneity.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 cell type sets

Platform:

GPL97

Series:

GSE4858

12 Samples

Download data

(Submitter supplied) Characterization of the transcriptional profile of SP human keratinocytes from primary cultures. Keywords: repeat sample

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1936

8 Samples

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(Submitter supplied) Background: Human interfollicular epidermis is sustained by the proliferation of stem cells and their progeny, transient amplifying cells. Molecular characterization of these two cell populations is essential for better understanding of self renewal, differentiation and mechanisms of skin pathogenesis. The purpose of this study was to obtain gene expression profiles of alpha 6+/MHCI+, transient amplifying cells and alpha 6+/MHCI- , putative stem cells, and to compare them with existing data bases of gene expression profiles for mouse hair follicle stem cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL96

4 Samples

Download data: CEL, CHP

(Submitter supplied) Transcriptional profiling of KP and DK through RNA-seq

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

6 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array; Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

4 related Platforms

20 Samples

Download data: BED, CEL, NARROWPEAK

(Submitter supplied) Gene expression profiling of progenitor and differentiated keratinocytes by Affymetrix microarray

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17180

6 Samples

Download data: CEL

(Submitter supplied) Genome-wide mapping of H3K4me1, H3K4me3 and H3K27ac in KP and DK

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL10999 GPL15520

7 Samples

Download data: BED, NARROWPEAK

(Submitter supplied) Investigation of promoters usage in KP cells

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL10999

1 Sample

Download data: BED

(Submitter supplied) Performing global gene expression profiling for different types of keratinocytes colonies can provide important information that might help in understanding the biology and ontogeny of epidermal skin stem cells. We used microarrays to detail the global gene expression profiling for different types of keratinocytes colonies including Holoclones (stem cells), Meroclones (transient amplifying cells), and Paraclones (differentiated cells) .

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17586

18 Samples

Download data: CEL, CHP

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Expression profiling by array

Platforms:

GPL21697 GPL28718

64 Samples

Download data: CEL, TXT

(Submitter supplied) Autologous epidermal cultures can permanently restore a functional epidermis on severely burned patients. Transgenic epidermal grafts do so also in genetic skin diseases as Junctional Epidermolysis Bullosa. Clinical success strictly requires an adequate number of epidermal stem cells, detected as holoclone-forming cells. To date, such cells can be only partially distinguished from the other transient amplifying clonogenic keratinocytes and cannot be prospectively isolated. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21697

4 Samples

Download data: TXT

(Submitter supplied) Autologous epidermal cultures can permanently restore a functional epidermis on severely burned patients. Transgenic epidermal grafts do so also in genetic skin diseases as Junctional Epidermolysis Bullosa. Clinical success strictly requires an adequate number of epidermal stem cells, detected as holoclone-forming cells. To date, such cells can be only partially distinguished from the other transient amplifying clonogenic keratinocytes and cannot be prospectively isolated. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL28718

60 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

10 Samples

Download data: CEL, CHP, TXT

(Submitter supplied) To identify genes expressed predominantly in the ventral skin epidermal basal cells of pregnant mice, we performed DNA microarray analysis by using FACS-purified epidermal basal cells from ventral skin at 0 and 16 dpc, and dorsal skin at 16 dpc.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL, CHP, TXT

(Submitter supplied) To identify genes expressed predominantly in the ventral skin dermis of pregnant mice, we performed DNA microarray analysis by using isolated dermal tissues from ventral skin at 0 and 15 dpc, PP2-injected ventral skin at 15 dpc, and dorsal skin at 15 dpc.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

4 Samples

Download data: CEL, TXT

(Submitter supplied) Infrequently (LRCs) and frequently (non-LRCs) dividing cells in the mouse epidermis are moleculary distinct.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

12 Samples

Download data: CEL

(Submitter supplied) Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare palatal epithelial basal cell transcriptomes between infrequently and frequently dividing cells (IDC/FDCs). Methods: RNA was isolated from 200-1000 palatal basal epithelial cells and libraries prepared using the SoLo RNA-Seq library preparation kit (NuGEN). more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

12 Samples

Download data: TXT