GEO DataSets

Analysis of lesional and non-lesional skin biopsy specimens from adult patients with atopic dermatitis (AD). Results provide insight into the molecular changes associated with early AD inflammation.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 development stage, 2 disease state, 11 individual sets

Platform:

GPL97

Series:

GSE5667

17 Samples

Download data: CEL

(Submitter supplied) Asthmatic chronic rhinosinusitis with nasal polyps (aCRSwNP) is a common disruptive eosinophilic disease. Therefore, we sought to identify gene expression changes in nasosinus inflamed mucosa and adjacent polyp tissue from subjects with aCRSwNP.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL5175

39 Samples

Download data: CEL

(Submitter supplied) Atopic dermatitis (AD) is a common pruritic dermatitis with macroscopically nonlesional skin that is often abnormal. Therefore, we used high-density oligonucleotide arrays to identify cutaneous gene transcription changes associated with early AD inflammation as potential disease control targets. Skin biopsy specimens analyzed included normal skin from five healthy nonatopic adults and both minimally lesional skin and nearby or contralateral nonlesional skin from six adult AD patients. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS2381 GDS2382

Platforms:

GPL97 GPL96

34 Samples

Download data: CEL

Analysis of lesional and non-lesional skin biopsy specimens from adult patients with atopic dermatitis (AD). Results provide insight into the molecular changes associated with early AD inflammation.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 development stage, 2 disease state, 11 individual sets

Platform:

GPL96

Series:

GSE5667

17 Samples

Download data: CEL

(Submitter supplied) Canine atopic dermatitis (AD) is clinically similar to human AD, implicating it as a useful model of human eosinophilic allergic disease. To identify cutaneous gene transcription changes in relatively early inflammation of canine AD, microarrays were used to monitor transcription in normal skin (n=13) and in acute lesional AD (ALAD) and nearby visibly nonlesional AD (NLAD) skin (n=13) from dogs. Scanning the putative abnormally-transcribed genes, several potentially relevant genes, some abnormally transcribed in both NLAD and ALAD (e.g. more...

Organism:

Canis lupus familiaris

Type:

Expression profiling by array

Platform:

GPL3738

39 Samples

Download data: CEL

(Submitter supplied) Subcutaneous abdominal adipose tissue was obtained from female subjects undergoing plastic surgery in agreement with French laws on biomedical research. Stromal cells prepared from WAT were cultured for 13 days in a chemically defined medium. At day 13, 60–80% of cells were differentiated into lipid droplet-containing adipocytes. The cells were infected at a m.o.i. of 200 for 6 h. The day after infection, cells were treated with the following drugs at 1 µM unless otherwise indicated: rosiglitazone (BRL49653, Smith Kline and French, Harlow, UK) and 9-cis-RA (Sigma). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL3941 GPL3942

16 Samples

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(Submitter supplied) Purpose: provide evidence that RNA-seq can add information to transcriptome profiling already discovered by other technologies for atopic dermatitis Methods: mRNA profiles of 20 atopic dermatitis were analyzed to compare lesional and non-lesional skin, then transcriptomes found by reads were compared to Microarray and RT-PCR Results:RNA-seq provided complementary genes to AD transcriptome IL-36 and TREM-1 Conclusions: Our study represents the first analysis of lesional AD tissue by RNA-seq and comparison to microarray and RT-PCR

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL10999

40 Samples

Download data: TXT

(Submitter supplied) Background: Alternative polyadenylation (APA) is emerging as a widespread mechanism of gene regulation. The usage of APA sites allows a single gene to encode multiple mRNA transcripts with different 3'-untranslated region (3'UTR) lengths. Many disease processes reflect the importance of the regulation of APA site switching. The objective of this study was to explore the profiling of tandem APA sites in nasal polyps compared with nasal uncinate process mucosa. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL9115

4 Samples

Download data: TXT

(Submitter supplied) This is the first report that establishes robust epidermal and dermal genomic signatures of lesional and nonlesional AD skin and normal skin compared with whole tissues. These data establish the utility of laser capture microdissection to separate different compartments and cellular subsets in patients with AD, allowing localization of key barrier or immune molecules and enabling detection of gene products usually not detected on arrays.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

52 Samples

Download data: CEL

(Submitter supplied) Genome-Wide Profiling of Lesional and Non-Lesional Skin from Atopic Dermatitis, Psoriasis, and Contact Dermatitis Skin

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

75 Samples

Download data: TXT

(Submitter supplied) The aim of this study was to find disease-associated genes in atopic eczema. Keywords: Skin biopsy, DNA microarray, atopic eczema

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL96

20 Samples

Download data

(Submitter supplied) Recently, it was shown that lesional skin of atopic dermatitis patients expresses low levels of some antimicrobial peptides, compared with psoriasis patients. Here we performed microarray analysis on mRNA from purified lesional epidermal cells of patients with chronic plaque psoriasis and chronic atopic dermatitis, to investigate whether this is a general phenomenon for host defense proteins, and how specific it is for this class of molecules. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4695

6 Samples

Download data: GPR, TIFF

(Submitter supplied) Atopic dermatitis (AD) is a common inflammatory skin disease with underlying defects in epidermal function and immune responses. The goal of this study was to investigate differences in gene expression in lesional skin from patients with mild extrinsic or intrinsic AD compared to skin from healthy controls and from lesional psoriasis skin. The aim was to identify differentially expressed genes involved in skin barrier formation and inflammation, and to compare our results with those reported for patients with moderate and severe AD.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17692

31 Samples

Download data: CEL

(Submitter supplied) Genes involved in the inflammatory response resulting in allergic contact dermatitis (ACD) are only partly known. In this study, we introduce the use of high density oligonucleotide arrays for gene expression profiling in human skin during the elicitation of ACD. Skin biopsies from normal and nickel-exposed skin were obtained from 7 nickel-allergic patients and 5 non-allergic controls at four different time points during elicitation of eczema: 0h, 7h, 48h and 96h. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2935

Platform:

GPL570

34 Samples

Download data: CEL

Analysis of skin biopsies from nickel-allergic patients whose skins were exposed to nickel, a sensitizing hapten, for up to 96 hours to elicit allergic contact dermatitis (ACD). Results provide insight into molecular mechanisms underlying the pathogenesis of ACD.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent, 2 disease state, 12 individual, 4 time sets

Platform:

GPL570

Series:

GSE6281

34 Samples

Download data: CEL

(Submitter supplied) Background: Atopic dermatitis (AD) predominantly affects young children, but our understanding of AD pathogenesis is based on skin and blood samples from longstanding adult AD. Genomic biopsy profiling from early pediatric AD showed significant Th2 and Th17/Th22-skewing, without the characteristic adult Th1 up-regulation. Since obtaining pediatric biopsies is difficult, blood gene expression profiling may provide a surrogate for the pediatric skin signature. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

46 Samples

Download data: CEL

(Submitter supplied) Background: Atopic eczema (AE) is a common chronic inflammatory skin disorder. In order to dissect the genetic background several linkage and genetic association studies have been performed. Yet very little is known about specific genes involved in this complex skin disease, and the underlying molecular mechanisms are not fully understood. Results: We used human DNA microarrays to identify a molecular picture of the programmed responses of the human genome to AE. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL7191

30 Samples

Download data

(Submitter supplied) To obtain a detailed molecular understanding of both the temporal and spatial variation in atopic dermatitis (AD), we conducted a longitudinal case-control study, in which we followed a population, the Gentofte AD (GENAD) cohort, of mild-to-moderate AD patients and matched healthy controls for more than a year. By the use of 1.5 mm punch biopsies, we obtained 393 samples from lesional, non-lesional and healthy skin, from multiple anatomic regions at different time points for transcriptomic profiling. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

339 Samples

Download data: CSV

(Submitter supplied) Clinical overlaps between psoriasis and atopic dermatitis are sometimes undiscernible, and there is no consensus whether to treat the overlap phenotype as psoriasis or atopic dermatitis. We enrolled patients diagnosed with either psoriasis or atopic dermatitis, and clinically re-stratified them into classic psoriasis, classic atopic dermatitis, and the overlap phenotype between psoriasis and atopic dermatitis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

75 Samples

Download data: CEL

(Submitter supplied) Both agonist studies and loss-of-function models indicate that PPARγ plays an important role in cutaneous biology. Since PPARγ has a high level of basal activity, we hypothesized that epidermal PPARγ would regulate normal homeostatic processes within the epidermis. In this current study, we performed mRNA sequencing and differential expression analysis of epidermal scrapings from knockout mice and wildtype littermates. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

12 Samples

Download data: TXT