GEO DataSets

Analysis of hypoxic fibroblasts bearing deletions in the CH1 domains of 1 allele of p300 and 2 alleles of CBP. CH1 domains interact with the C-TAD domain of hypoxia-inducible transcription factors HIF-1a and -2a. Results provide insight into the role of CH1 domains in the hypoxia response.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation, 2 stress sets

Platform:

GPL339

Series:

GSE3195

4 Samples

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(Submitter supplied) The C-terminal activation domain (C-TAD) of the hypoxia-inducible transcription factors HIF-1? and HIF-2? binds the CH1 domains of the related transcriptional coactivators CREB-binding protein (CBP) and p300, an oxygen-regulated interaction thought to be highly essential for hypoxia-responsive transcription. The role of the CH1 domain in vivo is unknown, however. We created mutant mice bearing deletions in the CH1 domains (?CH1) of CBP and p300 that abrogate their interactions with the C-TAD, revealing that the CH1 domains of CBP and p300 are genetically non-redundant and indispensable for C-TAD transactivation function. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL1261 GPL339

32 Samples

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(Submitter supplied) The CH1 protein interaction domain of the transcriptional coactivators p300 and CBP is thought to interact with HIF-1alpha and this interaction is thought to be critical to the expression of HIF-1alpha target genes in response to hypoxia. Trichostatin A (TSA), an inhibitor of histone deacetylases, has been reported to repress the expression of HIF-1alpha target genes. To test the requirement of the CH1 domain and TSA for gene expression in response to dipyridyl (a hypoxia mimetic), primary mouse embryonic fibroblasts (MEFs) were generated from C57Bl/6x129/Sv F2 e14.5 embryos that contain a deletion in the CH1 domain of three of four alleles of CBP and p300. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2162

Platform:

GPL1261

16 Samples

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(Submitter supplied) The CH1 protein interaction domain of the transcriptional coactivators p300 and CBP is thought to interact with HIF-1alpha and this interaction is thought to be critical to the expression of HIF-1alpha target genes in response to hypoxia. To test the requirement of the CH1 domain for gene expression in response to hypoxia, rimary mouse embryonic fibroblasts (MEFs) were generated from C57Bl/6x129/Sv F2 e14.5 embryos that contain a deletion in the CH1 domain of three of four alleles of CBP and p300. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2161

Platform:

GPL1261

12 Samples

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(Submitter supplied) The CH1 (TAZ) domain of the transcriptional coactivators p300 and CBP has been reported to interact with the transcription factor HIF-1alpha and this interaction is thought to be critical for HIF-1alpha target gene expression in response to hypoxia. To determine the requirement for the CH1 domain in hypoxia-responsive gene expression, primary mouse embryonic fibroblasts (MEFs) were generated from e14.5 C57B/6x129/Sv F2 embryos that were either wildtype or bore deletion mutations in the CH1 protein binding domains of both alleles of p300 and one allele of CBP (tri_CH1). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2160

Platform:

GPL339

4 Samples

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Analysis of trichostatin A (TSA)-treated hypoxic fibroblasts with deletions in CH1 domains of p300 and CBP, and a p300 or CBP allele knockout. TSA is a histone deacetylase inhibitor. Results provide insight into the role of deacetylase activity in CH1-independent hypoxia inducible transcription.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 agent, 4 genotype/variation sets

Platform:

GPL1261

Series:

GSE3296

16 Samples

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Analysis of hypoxic fibroblasts with deletions in the CH1 domains of p300 and CBP combined with a p300 or CBP allele knockout. CH1 domains interact with the C-TAD domain of hypoxia-inducible transcription factors HIF-1a and -2a. Results provide insight into the role for CH1 domains in hypoxia.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 genotype/variation, 2 stress sets

Platform:

GPL1261

Series:

GSE3196

12 Samples

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(Submitter supplied) CHD4 coactivates a subset of HIF target genes in breast cancer MDA-MB-231 cells.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

8 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL11180 GPL11002

20 Samples

Download data: BW, CEL

(Submitter supplied) Genome-wide distribution of histone H3K18 and H3K27 acetyltransferases, Crebbp (CBP) and Ep300 (p300), is used to map enhancers and promoters, but whether these elements functionally require CBP/p300 remains largely uncertain. We investigated this relationship by comparing genomic CBP recruitment with gene expression in wild type and CBP/p300 double-knockout fibroblasts. ChIP-seq revealed nearby CBP recruitment for 20 percent of constitutively expressed genes, but surprisingly, three-quarters of these were unaffected or slightly activated by CBP/p300 deletion. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL11002

8 Samples

Download data: BW

(Submitter supplied) Genome-wide distribution of histone H3K18 and H3K27 acetyltransferases, Crebbp (CBP) and Ep300 (p300), is used to map enhancers and promoters, but whether these elements functionally require CBP/p300 remains largely uncertain. We investigated this relationship by comparing genomic CBP recruitment with gene expression in wild type and CBP/p300 double-knockout fibroblasts. ChIP-seq revealed nearby CBP recruitment for 20 percent of constitutively expressed genes, but surprisingly, three-quarters of these were unaffected or slightly activated by CBP/p300 deletion. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11180

12 Samples

Download data: CEL

(Submitter supplied) The activation of stress response pathways in synovial fibroblasts (SF) is a hallmark of rheumatoid arthritis (RA). CBP and p300 are two highly homologous histone acetyl transferases and writers of activating histone 3 lysine 27 acetylation (H3K27ac) marks. We investigated individual functions of CBP and p300 using a silencing strategy, followed by RNA-sequencing, and pathway enrichment analysis. We have selected stress-related pathways for a further in-depth investigation of individual functions of CBP and p300 in SF. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24676

36 Samples

Download data: TXT

(Submitter supplied) We performed gene expression profiling of oligooxopiperazines (OPs) targeting the hypoxia-inducible transcription factor complex. Treatment of cells with OPs inhibited hypoxia-inducible gene expression in A549 cells.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5067

Platform:

GPL6244

15 Samples

Download data: CEL

Analysis of hypoxic A549 cells treated with oligooxopiperazines (OOPs) BB2-125, BB2-162, and BB2-282. OOPs are designed mimics of a key α-helical domain at the interface of hypoxia-inducible factor 1α (HIF1α) and coactivator p300. Results provide insight into the effect of OOPs on HIF signaling.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 4 agent, 2 stress sets

Platform:

GPL6244

Series:

GSE48134

15 Samples

Download data: CEL

(Submitter supplied) We performed gene expression profiling of hydrogen-bond surrogate that targets hypoxia-inducible transcription factior complex and results in inhibition of hypoxia-inducible genes with relatively minimal perturbation of non-targeted signaling pathways.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

12 Samples

Download data: CEL